This experiment was designed to study the effects of lipid culture on INS-1 derived glucose-responsive 832/13 cells. After 48-hr culture with lipids, a dramatic decrease in glucose-responsiveness is observed. In order to detect some earlier changes, RNA was also collected at earlier time points (12hr and 24hr). To compensate for any changes associated with cell density, control cells (1%BSA culture) were included for each time point. RNA was prepared from INS-1 cells in Dr. Chris Newgard's lab, quantified and sent frozen in water. Six biological replicates were sent for the following conditions: (i) 1%BSA (12, 24, and 48 Hours) (ii) 1mM-Oleate/Palmitate (12, 24, and 48 Hours)

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• INS1: 890, 930, 990, 2002, 3627,
• insulin secretion: 550, 930, 990, 1030, 1050, 2160, 3042, 3546, 3650, 3980,