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BCBC Protocol Collection

The BCBC Protocol Collection is a resource for the aggregation and dissemination of protocols relevant to beta cell biology.

Recent additions to the collection

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  BCBC 3.0 Investigators & Co-Investigators 52
  BCBC 3.0 Affiliates 51
  Alumni Investigators 14
Your access level Public 14

ID Name Categories Access
4124 Assessment of vascularization of islet grafts Immunostaining Restricted
4163 Assessment of vascularization of islet grafts via Lectin infusion Beta cell imaging Restricted
1601 Cell Culture in Three-dimensional Synthetic PEG Hydrogels Primary culture Viewable
Request
4136 Chemical Differentiation of Human Pluripotent Stem Cells to Definitive Endoderm Using a Potent Small Molecule Inhibitor of GSK-3 ESC directed differentiation Restricted
2882 Co-culturing endothelial cell lines with embryonic pancreas explants Primary culture Viewable
Request
2862 Culturing Different Endothelial Cell Lines Primary culture Viewable
Request
4250 Differentiation of hESCs towards pancreatic like glucose-responsive insulin-secreting cells, in vitro ESC directed differentiation Restricted
1546 Directed differentiation of human embryonic stem cell to foregut endoderm ESC directed differentiation Viewable
Request
1544 Directed differentiation of mouse embryonic stem cells to foregut endoderm ESC directed differentiation Viewable
1545 Directed differentiation of mouse embryonic stem cells to Sox17+ cells ESC directed differentiation Viewable
4122 FACS of Human Islet Cell Types Cell sorting Restricted
4139 FGF2 Semb hESC differentiation protocol ESC directed differentiation Restricted
4137 Fibronectin and Vitronectin Improve Differentiation of Human Pluripotent Stem Cells to Definitive Endoderm ESC directed differentiation Restricted
4330 Frozen Section Staining_Avidin/Biotin Amplification Immunostaining Restricted
4167 Frozen Section Staining (Indirect Immunofluorescence) Immunostaining Restricted
4331 Frozen Section Staining_Tyramide Signal Amplification Immunostaining Restricted
1641 Generate insulin-secreting islet clusters from human iPS ESC directed differentiation Viewable
Request
4263 Human and Rodent Ocular Transplantation Beta cell imaging Restricted
4291 Islet Transplantation Procedure Dissection Restricted
1581 Isolation of different types of adult human pancreatic cells Cell sorting Viewable
Request
4243 Isolation of Pancreatic Islets from Zebrafish Purification
Beta cell imaging 		Restricted
4280 Isolation of Single Human Beta Cells - Identifying Somatic Mutations Primary culture Restricted
2662 Manual dissection of E10.5 pancreatic buds Dissection
Cell sorting 		Viewable
Request
4138 Melton hESC differentiation protocol ESC directed differentiation Restricted
4273 Methods for accurate cell tracking in movies of dividing progenitors Beta cell imaging
Primary culture
Immunostaining
Dissection 		Restricted
4222 Modified RNA Synthesis RNA prep. and purification Restricted
2642 Mouse genotyping PCR method Viewable
Request
4569 Non-Human Islet Perifusion None specified Restricted
4168 Pancreas Dissociation for Virus-Mediated Gene Transfer Dissection
Primary culture 		Restricted
4141 Pancreatic differentiation of hESCs Keller ESC directed differentiation Restricted
4106 Preparation and size-enrichment of secreted proteins from mammalian cells None specified Restricted
4293 Preparing Islets for Transplant and Calculating IEQs Dissection Restricted
4258 Procedure for assessing gene expression in cell subsets from the human fetal pancreas. RNA prep. and purification Restricted
4257 Procedure for fractionating and isolating cell subsets from human fetal pancreas. Cell sorting Restricted
4260 Procedure for fractionation and gene expression analysis of juvenile human islet cells. PCR method
Cell sorting
RNA prep. and purification 		Restricted
4261 Procedure for identifying chromatin in juvenile human islet cells. Purification Restricted
4259 Procedures to assess gene expression changes in mouse fetal pancraetic cell subsets. Cell sorting
RNA prep. and purification
PCR method 		Restricted
4570 Procurement and shipment of human fetal pancreatic fragments None specified Restricted
4294 Procurement, Shipping, and Pathogen Testing of Fetal Pancreata Dissection Restricted
4105 Protocol for preparing and size-enrichment of secreted proteins None specified Restricted
4104 Protocol for preparing and size-enrichment of secreted proteins None specified Restricted
2982 Purification of MafA under Denaturing Conditions Purification Viewable
Request
4140 Sander monolayer CyT49 differentiation protocol ESC directed differentiation Restricted
1547 Standard mouse embryonic stem cell culture condition Primary culture Viewable
Request
3122 T1D Mouse Necropsy for Pancreas and Related Organs Beta cell imaging
Dissection
Immunostaining 		Viewable
4279 Tissue culture protocol for expansion of human gallbladder cells Primary culture
Dissection 		Restricted
4285 Tissue Culture Protocol for Expansion of Murine Gallbladder Cells Dissection
Primary culture 		Restricted
4165 Two-step crosslinking chromatin immunoprecipitation protocol Purification Restricted
4174 Using Ezh2 pathway inhibitor to increase pancreatic progenitor cells from endoderm ESC directed differentiation Restricted
4162 Vangl1 immunohistochemistry protocol Immunostaining Restricted
2922 Wholemount Immunofluorescence Immunostaining Viewable
Request
4166 Whole Mount Immunofluorescence Immunostaining Restricted

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