BCBC Protocol Collection
The BCBC Protocol Collection is a resource for the
aggregation and dissemination of protocols relevant to beta cell biology.
Recent additions to the collection
Your access level
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BCBC 3.0 Investigators & Co-Investigators |
31 |
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BCBC 3.0 Affiliates |
31 |
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Alumni Investigators |
14 |
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Public |
14 |
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4124
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Assessment of vascularization of islet grafts
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Immunostaining
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Aug 15, 2011
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 Restricted
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4163
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Assessment of vascularization of islet grafts via Lectin infusion
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Beta cell imaging
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Dec 13, 2011
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Restricted
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1601
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Cell Culture in Three-dimensional Synthetic PEG Hydrogels
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Primary culture
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Jun 10, 2009
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 Viewable
Request
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4136
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Chemical Differentiation of Human Pluripotent Stem Cells to Definitive Endoderm Using a Potent Small Molecule Inhibitor of GSK-3
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ESC directed differentiation
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Sep 30, 2011
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Restricted
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2882
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Co-culturing endothelial cell lines with embryonic pancreas explants
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Primary culture
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Jan 24, 2010
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Viewable
Request
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2862
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Culturing Different Endothelial Cell Lines
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Primary culture
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Jan 24, 2010
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Viewable
Request
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1546
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Directed differentiation of human embryonic stem cell to foregut endoderm
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ESC directed differentiation
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May 19, 2009
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Viewable
Request
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1544
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Directed differentiation of mouse embryonic stem cells to foregut endoderm
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ESC directed differentiation
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May 19, 2009
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Viewable
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1545
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Directed differentiation of mouse embryonic stem cells to Sox17+ cells
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ESC directed differentiation
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May 19, 2009
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Viewable
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4122
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FACS of Human Islet Cell Types
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Cell sorting
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Aug 3, 2011
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Restricted
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4139
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FGF2 Semb hESC differentiation protocol
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ESC directed differentiation
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Nov 4, 2011
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Restricted
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4137
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Fibronectin and Vitronectin Improve Differentiation of Human Pluripotent Stem Cells to Definitive Endoderm
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ESC directed differentiation
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Sep 30, 2011
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Restricted
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4167
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Frozen Section Staining (Indirect Immunofluorescence)
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Immunostaining
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Dec 19, 2011
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Restricted
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1641
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Generate insulin-secreting islet clusters from human iPS
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ESC directed differentiation
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Jun 26, 2009
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Viewable
Request
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1581
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Isolation of different types of adult human pancreatic cells
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Cell sorting
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Jun 5, 2009
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Viewable
Request
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4243
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Isolation of Pancreatic Islets from Zebrafish
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Purification
Beta cell imaging
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Jan 23, 2012
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Restricted
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2662
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Manual dissection of E10.5 pancreatic buds
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Cell sorting
Dissection
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Dec 16, 2009
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Viewable
Request
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4138
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Melton hESC differentiation protocol
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ESC directed differentiation
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Nov 4, 2011
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Restricted
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4222
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Modified RNA Synthesis
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RNA prep. and purification
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Jan 18, 2012
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Restricted
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2642
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Mouse genotyping
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PCR method
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Dec 16, 2009
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Viewable
Request
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- Protocols can be kept private or shared with others,
- We can assist with data submission and annotation,
- Submit laboratory-generated protocols,
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