Rosa26R26-228-DR5-TA-Cerulean - ES Cell Line RES982
ESC Line Information
| Cell Line Name: | Rosa26R26-228-DR5-TA-Cerulean |
|---|---|
| Parental Cell Line: | TL1-Roas26[LCA] 5B9 |
| Background Strain: | 129 |
| Culturing Protocol: | Std_mESC_Culture.doc |
| Description: | In these cells Rosa26 gene sequences from -228 to +81 were replaced by a retinoic acid response element (DR5) fused to a TATA-Cerulean (CFP) reporter. These ESCs may be useful for assess retinoic acid responsiveness. |
Genetic Alterations
| 1) RMCE Targeted Mutagenesis | |
|---|---|
| Type of Allele | Cassette Acceptor |
| Targeted Gene | gene trap ROSA 26, Philippe Soriano (Gt(ROSA)26Sor - NCBI GeneID:14910) |
| Targeted Allele |
targeted mutation 1
(Rosa26tm1(LCA) - MGI:104735)
|
| Description of Targeting Vector | The Rosa 26 cassette acceptor allele was created by replacing a 5.165 kb region of this gene containing exon 1 with a floxed tk-neo cassette, a puromycin-delta-thymidine kinase fusion gene driven by the mouse phosphoglycerol kinase promoter (pU-deltaTK) and a neomycin resistant gene driven by the bacterial EM7 promoter (EM7neo) flanked by minimal (34 bp) tandemly oriented lox71 and lox2272 sites. |
| Targeting Vector Genbank File |
pRosa26.LCA.gb |
| Recombinase-Mediated Cassette Exchange Stage | |
| Type of Allele: | Gene Replacement |
| Exchanged Cassette Gene | tumor necrosis factor receptor superfamily, member 10b (DR5 - NCBI GeneID:21933) |
| Exchanged Cassette Allele Name | Roas26R26-228-DR5-TA-Cerulean |
| Description of Exchange Vector | not available |
| Exchange Vector Genbank File: | R26228DR5TACerulean.gb |
| Citations | Not Available |
Associated Images
| Image 1 | |
|---|---|
|
Description: In this experiment native Rosa26 gene sequences from -228 to +81 were replaced by a retinoic acid response element (DR5) fused to a TATA-Cerulean red reporter. These cells, which can be used to assess RA responsiveness, were used to test the feasibility of inserting signaling sentinel cassettes into a facilitating chromosomal locus. Reference: Not provided |
Repositories
| MMRRC | |
|---|---|
 Request via www.mmrrc.org website
|
Stock #: VUMC Availability Notes: Not provided |
Contact Information
| Preferred Contact | |
|---|---|
| Name | Mark Magnuson |
| Institution | Vanderbilt University |
| Phone | 615-322-7006 |
| mark.magnuson@vanderbilt.edu | |
| Primary Lab Contact | |
| Name | Susan Hipkens |
| Institution | Vanderbilt University |
| Phone | 615-343-7422 |
| susan.hipkens@vanderbilt.edu | |
Associated Publications
No publications associated
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Resource Tags
embryonic, es, esc, mESC Core, Rosa26R26-228-DR5-TA-Cerulean, stem, TL1-Roas26[LCA] 5B9
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