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Monoclonal Human Hu ES-Derived Early Endoderm raised in Mouse - Antibody RES941


Antibody Information

Antibody ID: AB2513
Antigen: Hu ES-Derived Early Endoderm (No Gene ID associated)
Type: Monoclonal
Isotype: IgG1
Immunogen Source: Cells
Raised In: Mouse
Peptide: Not provided
Source of Antigen: Human
Cross Reacts With: Human
Affinity Purified: Supernatant
Purity Details: Not provided
Positive Control: Human ES-Derived Early Endoderm
Notes: The following is a preliminary discussion of the specificity of the reported antibody. This antibody was raised against day 6 early endoderm generated from human ES cells. This antibody has been found to selectively react with a cell surface molecule on human ES-derived cultures enriched for early primitive streak and anterior primitive streak, and to a lesser extent on ES cells and definitive endoderm, and not with mesoderm.

Applications and Uses

Application Concentration Storage Buffer Protocols and Description
FACS Undiluted As provided Description: This antibody was raised against day 6 early endoderm generated from human ES cells. This antibody has been found to selectively react with a cell surface molecule on human ES-derived cultures enriched for early primitive streak and anterior primitive streak. Cells expressing the molecule can be detected by FACS.
Protocols:

Associated Images

Image 1
Description:
This figure illustrates staining of a subset of cells in human ES-derived cultures enriched for early primitive streak and anterior primitive streak. Cells were incubated with the monoclonal antibody, washed, then incubated with an allophycocyanin-conjugated anti-mouse-Ig.

Reference:
Not provided

Repositories

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Contact Information

Preferred Contact
Name Philip Streeter
Institution Oregon Health & Science University
Phone 503-494-1762
Email streetep@ohsu.edu

Associated Publications

No publications associated

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Access Status

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Primary contributor: Grompe Lab
Co-contributed by:

Resource Tags

antibody, Hu ES-Derived Early Endoderm, Human, Monoclonal

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Resource History & Actions

Approved on
Last modified on Feb 10, 2009
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