Monoclonal Mouse Mo ES-Derived Early Endoderm raised in Rat - Antibody RES901

Antibody Information

Antibody ID:	AB2473
Antigen:	Mo ES-Derived Early Endoderm (No Gene ID associated)
Type:	Monoclonal
Isotype:	IgG1
Immunogen Source:	Cells
Raised In:	Rat
Peptide:	Not provided
Source of Antigen:	Mouse
Cross Reacts With:	Mouse
Affinity Purified:	Supernatant
Purity Details:	Not provided
Positive Control:	ES-derived early endoderm
Notes:	This antibody was raised against Foxa2+Bry+ cells isolated from mouse ES cell cultures differentiated for 4 days in the presence of human Activin A (25-50 ng/ml) and mouse Wnt3a (3-5 ng/ml). This antibody has been shown to react with a cell surface molecule on a subset of mouse Foxa2+Bry+ cells, but has not been extensively evaluated on ES cells or ES cells differentiated to alternate lineages.

Application	Concentration	Storage Buffer	Protocols and Description
FACS	Undiluted	As provided	Description: This antibody reacts with a cell surface molecule on a subset of Foxa2+Bry+ cells derived from mouse ES cell cultures differentiated for 4 days in the presence of human Activin A (25-50 ng/ml) and mouse Wnt3a (3-5 ng/ml). Cells expressing the molecule can be detected by FACS. Protocols:

Image 1
	Description: This figure illustrates staining of a subset of Foxa2+Bry+ cells with this antibody. Cells were incubated with the monoclonal antibody, washed, then incubated with an allophycocyanin-conjugated donkey anti-rat-Ig. Reference: Not provided