Ghrl^Cre-eGFP - ES Cell Line RES761

ESC Line Information

Cell Line Name:	Ghrl^Cre-eGFP
Parental Cell Line:	TL1-Ghrl{LCA}
Background Strain:	129
Culturing Protocol:	Not provided.
Description:	Recombinase mediated cassette exchange (RMCE) was used to insert the Cre-eGFP fusion protein into the ghrelin locus using ES cells containing the Ghrl^LCA allele. The Cre-eGFP protein uses the endogenous AUG start codon and polyadenylation sequence.

1) RMCE Targeted Mutagenesis
Type of Allele	Cassette Acceptor
Targeted Gene	Ghrelin (Ghrl - NCBI GeneID:58991)
Targeted Allele	targeted mutation (Ghrl^LCA - MGI:1930008)
Description of Targeting Vector	Not available
Targeting Vector Genbank File	ghrelin.gb
Recombinase-Mediated Cassette Exchange Stage
Type of Allele:	Gene Replacement
Exchanged Cassette Gene	Cre-eGFP (Cre-eGFP)
Exchanged Cassette Allele Name	Ghrl^Cre-eGFP
Description of Exchange Vector	not available
Exchange Vector Genbank File:	Ghrl_Cre-eGFP_CEV.gb
Citations	Not Available

Image 1
	Description: Schematic diagram showing modifications of the ghrelin locus to insert the Cre-eGFP fusion protein coding sequence into the ghrlein locus. The top diagram shows creation of the Ghrl^LCA allele. ES cells containing the Ghrl^LCA allele were then used to generate the Cre-eGFP knockin allele as outlined in the lower diagram. Reference: Not provided