OE.Human.CDK6.Tetracycline Responsive Element (TRE) - Adenovirus RES4100
Over-expression Construct Information
| NCBI Gene Name | Cyclin-dependent kinase 6 |
|---|---|
| NCBI Gene Symbol | CDK6 |
| NCBI Gene ID | 1021 |
| Species | Human |
| Nucleotide Accession Number | NM_001259.6 |
| Promoter | Tetracycline Responsive Element (TRE) |
| Tag | HA |
| Mutation | Not provided |
| Comments | The tet response element (TRE) is placed upstream of the human cdk6 cDNA in this Ad5 backbone adenovirus. The cdk6 construct also contains a C-terminal HA tag. When human islets are co-transduced with the adenovirus that uses the CMV-driven tet-transactivator (TTA), the cdk6 expression assessed by both immunohistochemistry and immunoblot, using either cdk6 or HA antibodies, is abundant, and increases in a dose-related manner over 72 hours with doses beginning at 0.1 ug/ml, and increasing to 1 µg/ml. This construct was generated using pACCMVpLpA & pJM17 in HEK293 cells. |
Repositories
| Stewart Lab | |
|---|---|
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Stock #: Adv-Stewart-CDK6 Availability Notes: Not provided |
Contact Information
| Preferred Contact | |
|---|---|
| Name | Andrew Stewart |
| Institution | University of Pittsburgh |
| Phone | 412-648-8630 |
| stewarta@pitt.edu | |
| Primary Lab Contact | |
| Name | Karen Takane |
| Institution | University of Pittsburgh Medical Center |
| Phone | 412-6489718 |
| takane@dom.pitt.edu | |
Associated Publications
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