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Monoclonal Human Pancreatic exocrine cells raised in Mouse - Antibody RES339


Antibody Information

Antibody ID: AB2194
Antigen: Pancreatic exocrine cells (No Gene ID associated)
Type: Monoclonal
Isotype: IgG1
Immunogen Source: Enriched pancreatic islets
Raised In: Mouse
Peptide: Not provided
Source of Antigen: Human
Cross Reacts With: Human
Affinity Purified: Supernatant
Purity Details: Not provided
Positive Control: Acetone fixed frozen sections of adult human pancreas
Notes: The monoclonal antibody HPx1 is derived from hybridoma HIC0-3B3. The monoclonal antibody selectively reacts with human exocrine cells in human pancreas. Mice were immunized with enriched islets. These cell preparations contain low levels of contaminating exocrine and ductal cells. These antibodies are currently being characterized. As such, the information included here should be considered preliminary data. It is requested that users of this antibody share data with provider as a mechanism to rapidly assist in antibody characterization.

Applications and Uses

Application Concentration Storage Buffer Protocols and Description
FACS Undiluted Tissue culture media Description: Flow cytometric analysis of enzyme dispersed enriched human islets revealed that the monoclonal antibody HPx1 reacts with a cell surface molecule on exocrine cells.
Protocols:
  1. Flow Cytometry: Labeling of Cell Surface Molecules on Human Cells with Mouse Monoclonal Antibodies
IHC-AF Undiluted Tissue culture media Description: Not provided
Protocols:
  1. Immunofluoresence Detection of Mouse Monoclonal Antibodies on Sections of Acetone-Fixed Frozen Human Tissue

Associated Images

Image 1
Description:
Human pancreas frozen section illustrating HPx1 reactivity with exocrine cells. The monoclonal antibody was detected using a polyclonal Cy3-conjugated anti-mouse immunoglobulin (red). Cell nuclei were labeled with Hoeschst 33342 (blue).

Reference:
Not provided

Repositories

Streeter Lab
Out of stock Stock #: Not provided
Availability Notes: Requester is responsible shipping costs.

Contact Information

Not provided

Associated Publications

No publications associated

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Access Status

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Primary contributor: Grompe Lab
Co-contributed by:

Resource Tags

antibody, FACS, Human, Monoclonal, Pancreatic exocrine cells

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Resource History & Actions

Approved on
Last modified on Jun 08, 2010
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