Monoclonal Human Pancreatic endocrine cells raised in Mouse - Antibody RES326

Antibody Information

Antibody ID:	AB2115
Antigen:	Pancreatic endocrine cells (No Gene ID associated)
Type:	Monoclonal
Isotype:	IgG1
Immunogen Source:	Whole cells
Raised In:	Mouse
Peptide:	Not provided
Source of Antigen:	Human
Cross Reacts With:	Human
Affinity Purified:	Supernatant
Purity Details:	Not provided
Positive Control:	Acetone-fixed frozen tissue sections of adult human pancreas.
Notes:	The monoclonal antibody HPi1 is derived from hybridoma HIC0-4F9. The monoclonal antibody selectively reacts with a cell surface molecule on human endocrine cells in pancreas. Mice were immunized with enriched islets. These cell preparations contain low levels of contaminating exocrine and ductal cells. These antibodies are currently being characterized. As such, the information included here should be considered preliminary data. It is requested that users of this antibody share data with provider as a mechanism to rapidly assist in antibody characterization.

Application	Concentration	Storage Buffer	Protocols and Description
FACS	Undiluted	Tissue culture media	Description: Not provided Protocols: Flow Cytometry: Labeling of Cell Surface Molecules on Human Cells with Mouse Monoclonal Antibodies
IHC-AF	Undiluted	Tissue culture media	Description: Not provided Protocols: Immunofluoresence Detection of Mouse Monoclonal Antibodies on Sections of Acetone-Fixed Frozen Human Tissue

Image 1
	Description: Human pancreas frozen section illustrating HPi1 reactivity with endocrine cells. The monoclonal antibody was detected using a polyclonal Cy3-conjugated anti-mouse immunoglobulin (red). Cell nuclei were labeled with Hoeschst 33342 (blue). Reference: Not provided

Streeter Lab
Request this resource	Stock #: HPi1 Availability Notes: Available at OHSU, Stock #1038A

Preferred Contact
Name	Markus Grompe
Institution	Oregon Health & Science University
Phone	503-494-6888
Email	grompem@ohsu.edu
Primary Lab Contact
Name	Not provided
Institution	Not provided
Phone	Not provided
Email	Not provided

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Primary contributor: Grompe Lab
Co-contributed by:

antibody, FACS, Human, Monoclonal, Pancreatic endocrine cells

Approved on
Last modified on Jul 14, 2006

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