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Monoclonal Rat Nkx6.1 raised in Mouse - Antibody RES308


Antibody Information

Antibody ID: AB2022
Antigen: Nkx6.1 (NCBI Gene ID: 65193)
Type: Monoclonal
Isotype: IgG1
Immunogen Source: Fusion Protein
Raised In: Mouse
Peptide: GST-Nkx6.1(aa299-365)
Source of Antigen: Rat
Cross Reacts With: Mouse,Rat
Affinity Purified: Affinity Purified
Purity Details: Not provided
Positive Control: Adult mouse pancreas
Notes: Does not cross-react with human

Applications and Uses

Application Concentration Storage Buffer Protocols and Description
WB 0.5 ug/ml PBS, 0.05% Sodium Azide Description: Not provided
Protocols:
    Not provided
IHC-AlFr 1:1000 PBS, 0.05% Sodium Azide Description: Not provided
Protocols:
  1. TSA staining protocol

Associated Images

Image 1
Description:
IHC staining of adult mouse pancreas visualized with Cy3 Fluorophore Tyramide Amplification agent.

Reference:
Not provided
Image 2
Description:
Triple staining of adult mouse pancreas with anti-Nkx6.1 F65A2 (red) anti-isulin (green) and anti-glucagon (blue) visualized with confocal microscopy.

Reference:
Not provided
Image 3
Description:
Western blot with mAb F65A2. Lane 1. SeeBlue Plus2 pre-staind Standard. Lane 2. GST-Nkx6.1(aa299-365). Lane 3. GST-Nkx6.2(aa208-277)

Reference:
Not provided

Repositories

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DSHB - Madsen
Request via dshb.biology.uiowa.edu/Welcome?search=madsen website Stock #: F65A2
Availability Notes: Supplied in 100 microgram aliquots

Contact Information

Preferred Contact
Name Signe Ingvarsen
Institution Novo Nordisk A/S
Phone +45 30750390
Email sgi@novonordisk.com

Associated Publications

Publication Citation
16401696 Pedersen IL, Klinck R, Hecksher-Sorensen J, Zahn S, Madsen OD, Serup P, Jorgensen MC Generation and characterization of monoclonal antibodies against the transcription factor Nkx6.1. (2006) J Histochem Cytochem 54: 567-74 (Added January 08, 2013)

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Access Status

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Resource Tags

AbCore, antibody, DSHB, Monoclonal, Nkx6.1, Rat

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Resource History & Actions

Approved on
Last modified on Jan 07, 2010
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