Monoclonal Mouse Ngn3 raised in Mouse - Antibody RES304
Antibody Information
| Antibody ID: | AB2012 |
|---|---|
| Antigen: | Ngn3 (NCBI Gene ID: 11925) |
| Type: | Monoclonal |
| Isotype: | IgG1 |
| Immunogen Source: | Fusion Protein |
| Raised In: | Mouse |
| Peptide: | GST-mNgn3(aa1-aa95) |
| Source of Antigen: | Mouse |
| Cross Reacts With: | Mouse |
| Affinity Purified: | Affinity Purified |
| Purity Details: | Not provided |
| Positive Control: | Mouse Pancreas |
| Notes: | Not provided |
Applications and Uses
| Application | Concentration | Storage Buffer | Protocols and Description |
|---|---|---|---|
| WB | 0.5 ug/ml | PBS, 0.05% Sodium Azide |
Description: Not provided Protocols:
|
| IHC-AlP | 1:100 - 1:500 dilutions | PBS with 0.05% Sodium Azide |
Description: IHC staining is performed with TSA, as staining without gives a very weak signal Protocols: |
Associated Images
| Image 1 | |
|---|---|
|
Description: IHC staining of Mouse Pancreas with anti-mNgn3 mAb F15A6 using TSA amplification Reference: Not provided |
| Image 2 | |
|
Description: WB with anti-Ngn3 mAb F15A6 Reference: Not provided |
Repositories
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Contact Information
| Preferred Contact | |
|---|---|
| Name | Signe Ingvarsen |
| Institution | Novo Nordisk A/S |
| Phone | +45 30750390 |
| sgi@novonordisk.com | |
Associated Publications
No publications associated
Comments
03/29/2005 06:55 AMInger Lund Pedersen |
For E14.5d mouse pancreas, the IF-TSA (from Molecular Probes) approach much better than conventional IF (using the protocol available from the BCBC website); 1:800 dilution optimal with TSA. Low, but effective detection of NGN3 + nuclei. Requires unusually high Ab concentration for TSA protocol. Ranks third of the three new monoclonal anti-NGN3?s. |
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