Polyclonal Mouse Ngn3 raised in Rabbit - Antibody RES303

Antibody Information

Antibody ID:	AB2011
Antigen:	Ngn3 (NCBI Gene ID: 11925)
Type:	Polyclonal
Isotype:	Not Applicable
Immunogen Source:	Fusion Protein
Raised In:	Rabbit
Peptide:	GST-mNgn3(aa1-aa95)
Source of Antigen:	Mouse
Cross Reacts With:	Mouse
Affinity Purified:	Serum
Purity Details:	Not provided
Positive Control:	Mouse Pancreas
Notes:	Not provided

Applications and Uses

Application	Concentration	Storage Buffer	Protocols and Description
IHC-AlP	1:500 dilution	Not provided	Description: IHC staining is performed with TSA, as staining without gives a very weak signal Protocols: IHC staining with TSA

Associated Images

Image 1
	Description: Not provided Reference: Not provided
Image 2
	Description: E10.5 mouse pancreas with anti-Pdx (green) and anti-Ngn3 (red) 1:2000 without TSA. Reference: Jonas Ahnfelt-R?nne, Hagedorn Research Institute
Image 3
	Description: E10.5 mouse ventral neural tube anti-Ngn3 (red) 1:2000 without TSA. Reference: Jonas Ahnfelt-R?nne, Hagedorn Research Institute

Repositories

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Contact Information

Preferred Contact
Name	Signe Ingvarsen
Institution	Novo Nordisk A/S
Phone	+45 30750390
Email	sgi@novonordisk.com

Associated Publications

Publication	Citation
17007831	Bjerknes M, Cheng H Neurogenin 3 and the enteroendocrine cell lineage in the adult mouse small intestinal epithelium. (2006) Dev Biol 300: 722-35 (Added July 30, 2010)

Comments

03/29/2005 06:54 AM
Inger Lund Pedersen

For E14.5d mouse pancreas, the IF-TSA protocol(from Molecular Probes) better than conventional IF(using the protocol available from the BCBC website); 1:400 dilution optimal with TSA. High overall background, but clear and strong nuclear signals of central epithelial structures.

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Primary contributor: BCBC Antibody Core

Resource Tags

AbCore, antibody, Mouse, Ngn3, Polyclonal

Resource History & Actions

Approved on
Last modified on Nov 09, 2010

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