Transcriptome profiling of e11.25 mouse pancreas: comparison between Ngn3-/- and Ngn3-/- ; Pdx1::Ngn3ER TM pancreatic epithelium. - Study GBCO3733

Genomics Study Specifications

Study Name

Transcriptome profiling of e11.25 mouse pancreas: comparison between Ngn3-/- and Ngn3-/- ; Pdx1::Ngn3ER TM pancreatic epithelium.

Contact Name

Anne Grapin-Botton (ISREC (Swiss Institute for Experimental Cancer Research) EPFL (Ecole Polytechnique Federale de Lausanne) EPFL SV ISREC - UPGRA)

Publication

Not provided

My Strategies

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Classification

Targets and roles of transcriptional regulators; Pancreas development and growth

Links

Biomaterials Graph

BCBC Release Date

January 19, 2010

Public Release Date

July 29, 2011

Citation

unavailable

Synopsis

Study Description
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Individual pancreatic buds of crosses between Ngn3+/- and Ngn3+/- ; Pdx1::Ngn3ER TM -IRES-GFP were dissected. Pregnant females were injected intra-peritoneal with 2mg of Tamoxifen at e10.25 (9 am) and dissected at e11.25 (9 to 11 am). Most of the mesenchymal tissue was removed to reduce the tissue complexity of the sample and enrich in epithelial pancreas progenitors. Moreover, at that developmental stage, Pdx1 promoter exhibit strong and homogenous expression levels. Rescuing Ngn3 expression only 24 hours before dissection (12h before activity, due to the time to process tamoxifen into the active compound 4OH tamoxifen) enriches the gene list in direct targets of Ngn3.

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