Rosa26R26-60-6XNotch-TA-Cerulean - ES Cell Line RES2802
ESC Line Information
| Cell Line Name: | Rosa26R26-60-6XNotch-TA-Cerulean |
|---|---|
| Parental Cell Line: | TL1-Rosa26(LCA) 5B9 |
| Background Strain: | 129 |
| Culturing Protocol: | Std_mESC_Culture.doc |
| Description: | The binding site for Rbpj, the transcriptional mediator of Notch signaling was inserted into the Rosa 26 locus replacing nucleotides -60 to -228. The complete insert contains the Rbpj cis element in a series of six repeats. Each repeat includes a 31 base-pair region derived from the CBF1/Rbpj binding region of the C-promoter of EBV (cataaattTTTTCCCACGgcgtgtttacacc). Uppercase letters are the consensus Rbpj binding sequence within the 31-bp element) (Hsieh et al., MCB 16:952, 1996)."
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Genetic Alterations
| 1) RMCE Targeted Mutagenesis | |
|---|---|
| Type of Allele | Cassette Acceptor |
| Targeted Gene | gene trap ROSA 26, Philippe Soriano (Gt(ROSA)26Sor - NCBI GeneID:14910) |
| Targeted Allele |
targeted mutation 1
(Rosa26tm1(LCA) - MGI:104735)
|
| Description of Targeting Vector | The Rosa 26 cassette acceptor allele was created by replacing a 5.165 kb region of this gene containing exon 1 with a floxed tk-neo cassette, a puromycin-delta-thymidine kinase fusion gene driven by the mouse phosphoglycerol kinase promoter (pU-deltaTK) and a neomycin resistant gene driven by the bacterial EM7 promoter (EM7neo) flanked by minimal (34 bp) tandemly oriented lox71 and lox2272 sites. |
| Targeting Vector Genbank File |
pRosa26.LCA.gb |
| Recombinase-Mediated Cassette Exchange Stage | |
| Type of Allele: | Gene Replacement |
| Exchanged Cassette Gene | Not provided. |
| Exchanged Cassette Allele Name | Rosa26(R26-60-6XNotch-TA-Cerulean) |
| Description of Exchange Vector | not available |
| Exchange Vector Genbank File: | 6xnotchrosa2660.txt |
| Citations | Not Available |
Associated Images
| Image 1 | |
|---|---|
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Description:
Conceptual nucleotide sequence of the cassette for the RMCE of a synthetic Notch-signal responsive promoter for insertion/replacement into the Rosa26-LCA. This replaces native Rosa26 gene sequences from -60 to +81 with the Notch responsive promoter. These ES cells may be useful to monitor Notch signaling. Light blue: Rosa26-60 Red: 6XNotch Dark green: Cerulean Light green: Ampicillin Blue: Vector Black: lox Reference: Not provided |
Repositories
| BCBC Mouse / ES Cell Core | |
|---|---|
 Request this resource
|
Stock #: BCBC2802 Availability Notes: Not provided |
Contact Information
| Preferred Contact | |
|---|---|
| Name | Ray MacDonald |
| Institution | University of Texas Southwestern Medical Center |
| Phone | 214-648-1923 |
| raymond.macdonald@utsouthwestern.edu | |
| Primary Lab Contact | |
| Name | Yanjie Chang |
| Institution | Not provided |
| Phone | Not provided |
| yanjie.chang@utsouthwestern.edu | |
Associated Publications
No publications associated
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