Rosa26R26-228-AR8-TA-mCherry - ES Cell Line RES2782
ESC Line Information
| Cell Line Name: | Rosa26R26-228-AR8-TA-mCherry |
|---|---|
| Parental Cell Line: | Rosa26{LCA} |
| Background Strain: | 129 |
| Culturing Protocol: | std_mesc_culture.doc |
| Description: | In these cells Rosa26 gene sequences from -228 to +81 were replaced by an Activin response element (AR8) fused to a TATA-mCherry reporter. These ESCs may be useful to assess Activin, Nodal, and TGFbeta responsiveness. |
Genetic Alterations
| 1) RMCE Targeted Mutagenesis | |
|---|---|
| Type of Allele | Cassette Acceptor |
| Targeted Gene | gene trap ROSA 26, Philippe Soriano (Gt(ROSA)26Sor - NCBI GeneID:14910) |
| Targeted Allele |
targeted mutation 1
(Rosa26tm1(LCA) - MGI:104735)
|
| Description of Targeting Vector | The Rosa 26 cassette acceptor allele was created by replacing a 5.165 kb region of this gene containing exon 1 with a floxed tk-neo cassette, a puromycin-delta-thymidine kinase fusion gene driven by the mouse phosphoglycerol kinase promoter (pU-deltaTK) and a neomycin resistant gene driven by the bacterial EM7 promoter (EM7neo) flanked by minimal (34 bp) tandemly oriented lox71 and lox2272 sites. |
| Targeting Vector Genbank File |
pRosa26.LCA.gb |
| Recombinase-Mediated Cassette Exchange Stage | |
| Type of Allele: | Gene Replacement |
| Exchanged Cassette Gene | Activin Response Element (R26-228-AR8-TA-mCherry) |
| Exchanged Cassette Allele Name | Rosa26tm2.2(R26-228-AR8-TA-mCherry) |
| Description of Exchange Vector | not available |
| Exchange Vector Genbank File: | pr26228_ar8_tacherry.gb |
| Citations | Not Available |
Associated Images
| No associated images have been supplied | |
Repositories
| MMRRC | |
|---|---|
 Request via www.mmrrc.org website
|
Stock #: Not provided Availability Notes: Not provided |
| BCBC Mouse / ES Cell Core | |
|---|---|
|
Request this resource
|
Stock #: BCBC2782 Availability Notes: Not provided |
Contact Information
| Preferred Contact | |
|---|---|
| Name | Palle Serup |
| Institution | Hagedorn Research Institute |
| Phone | +45 4443 9822 |
| pas@novonordisk.com | |
| Primary Lab Contact | |
| Name | Palle Serup |
| Institution | Hagedorn Research Institute |
| Phone | +45 4443 9822 |
| pas@novonordisk.com | |
Associated Publications
No publications associated
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