Polyclonal Rat PDX1 raised in Rabbit - Antibody RES271

Antibody Information

Antibody ID:	AB1067
Antigen:	PDX1 (NCBI Gene ID: 29535)
Type:	Polyclonal
Isotype:	Not Applicable
Immunogen Source:	Fusion Protein
Raised In:	Rabbit
Peptide:	Not provided
Source of Antigen:	Rat
Cross Reacts With:	Rat
Affinity Purified:	Serum
Purity Details:	Not provided
Positive Control:	Rat Pancreas or rat beta cell lines
Notes:	Not provided

Applications and Uses

Application	Concentration	Storage Buffer	Protocols and Description
IHC	1:500	PBS with azide	Description: Not provided Protocols: Fluorescence
IHC	1:5000	PBS with azide	Description: Not provided Protocols: Peroxidase

Associated Images

Image 1
	Description: NHI-6F-28 insulinoma cells (positive for Nkx 6.1 and Pdx-1 mRNA) were stained using antisera against Nkx 6.1 and Pdx-1. Both antisera were preabsorbed with either antigen (GST-Nkx 6.1, GST-Pdx-1) as indicated. As expected, only the corresponding antigen abolished staining. Identical results were obtained on pancreatic sections (not shown). Photomicrographs (A and D) were overexposed to visualize unstained nuclei. Reference: Jensen et al. 1996

Image 1

Description:

NHI-6F-28 insulinoma cells (positive for Nkx 6.1 and Pdx-1 mRNA) were stained using antisera against Nkx 6.1 and Pdx-1. Both antisera were preabsorbed with either antigen (GST-Nkx 6.1, GST-Pdx-1) as indicated. As expected, only the corresponding antigen abolished staining. Identical results were obtained on pancreatic sections (not shown). Photomicrographs (A and D) were overexposed to visualize unstained nuclei.

Reference:

Jensen et al. 1996

Repositories

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Contact Information

Preferred Contact
Name	Signe Ingvarsen
Institution	Novo Nordisk A/S
Phone	+45 30750390
Email	sgi@novonordisk.com

Associated Publications

Publication	Citation
11423476	Heller RS, Stoffers DA, Bock T, Svenstrup K, Jensen J, Horn T, Miller CP, Habener JF, Madsen OD, Serup P Improved glucose tolerance and acinar dysmorphogenesis by targeted (2001) Diabetes 50: 1553-61 (Added April 13, 2005)
8702531	Jensen J, Serup P, Karlsen C, Nielsen TF, Madsen OD mRNA profiling of rat islet tumors reveals nkx 6.1 as a beta-cell-specific homeodomain transcription factor. (1996) J Biol Chem 271: 18749-58 (Added May 15, 2013)

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Primary contributor: BCBC Antibody Core

Resource Tags

AbCore, antibody, PDX1, Polyclonal, Rat

Resource History & Actions

Approved on
Last modified on Oct 29, 2012

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