Rosa26MafA-Cherry - Mouse Strain RES2702
Mouse Information
| Common Name: | Rosa26MafA-Cherry |
|---|---|
| MGI Official Name: | Rosa26tm1.4(MafA-Cherry)Mgn |
| Description: | This mouse contains a bidirectional Tet0-regulated fusion gene that has been inserted into a disabled Rosa26 loxed cassette acceptor allele by RMCE. In one direction the tetO/CMV promoter drives expression of a red fluorescent protein (Cherry) while in the other direction it drives MafA expression. |
| Categories: |
Fluorescent Probes Tet |
Genetic Alterations
| 1) RMCE Targeted Mutagenesis | |
|---|---|
| Type of Allele | Cassette Acceptor |
| Targeted Gene | gene trap ROSA 26, Philippe Soriano (Gt(ROSA)26Sor - NCBI GeneID:14910) |
| Targeted Allele |
targeted mutation 1
(Rosa26tm1(LCA) - MGI:104735)
|
| Description of Targeting Vector | The Rosa 26 cassette acceptor allele was created by replacing a 5.165 kb region of this gene containing exon 1 with a floxed tk-neo cassette, a puromycin-delta-thymidine kinase fusion gene driven by the mouse phosphoglycerol kinase promoter (pU-deltaTK) and a neomycin resistant gene driven by the bacterial EM7 promoter (EM7neo) flanked by minimal (34 bp) tandemly oriented lox71 and lox2272 sites. |
| Targeting Vector Genbank File |
pRosa26.LCA.gb |
| Recombinase-Mediated Cassette Exchange Stage | |
| Type of Allele: | Conditional Activating |
| Exchanged Cassette Gene | v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian) (Mafa - NCBI GeneID:378435) |
| Exchanged Cassette Allele Name | Rosa26MafA-Cherry |
| Description of Exchange Vector | Through homologous recombination in ES cells, a 5.165 kb region of the Rosa26 gene containing exon 1 is replaced by a floxed tk-neo cassette, a puromycin-delta-TK fusion gene driven by the mouse phosphoglycerol kinase promoter (pUdelta-TK), and a neomycin reisitant gene driven by the bacterial EM7 promoter (EM7neo) flanked by minimal (34 bp) tandemly oriented lox71 and lox2272 sites (Cre-recombinase recognition sequences). |
| Exchange Vector Genbank File: | phygro66.2272.rv.mafa.cherry.gb |
| Citations | Not Available |
Strain Information
| Strain Type: | Mixed |
|---|---|
| Chimera/Founder Genetic Background: | 129S6/SvEvTac |
| Current Genetic Background: | Mixed 129S6 and C57BL6/J (date recorded: Not provided) |
| Strain Description: | Germline male chimeras are currently being backcrossed to C57BL6/J female mice. |
Associated Images
| Image 1 | |
|---|---|
|
Description: A bidirectional TetO-regulated fusion gene was inserted into a disabled Rosa26 loxed cassette acceptor allele by RMCE, as shown. The TetO-regulated reporter drives expression of RFP (mCherry) in one direction and MafA in the other. The pgk-hygromycin resistance cassette is flanked by tandem FRT sites to enable easy removal after germline transmission with FlpE. Reference: Not provided |
Repositories
| Magnuson Lab | |
|---|---|
 Request this resource
|
Stock #: VUMC Availability Notes: Not provided |
Contact Information
| Preferred Contact | |
|---|---|
| Name | Mark Magnuson |
| Institution | Vanderbilt University |
| Phone | 615-322-7006 |
| mark.magnuson@vanderbilt.edu | |
| Primary Lab Contact | |
| Name | Not provided |
| Institution | Not provided |
| Phone | Not provided |
| Not provided | |
Associated Publications
No publications associated
Comments
03/11/2012 02:34 PMMark Magnuson |
This mouse line was generated, but it was found to be very highly variegated at the outset and the allele ceased functioning within several generations. For this reason, the line was terminated. |
|
09/14/2011 01:38 PM Mark Magnuson |
This allele is highly variegated and appears to have undergone gene silencing after several generations of breeding. |
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