Sox17LCA - ES Cell Line RES263
ESC Line Information
| Cell Line Name: | Sox17LCA |
|---|---|
| Parental Cell Line: | TL-1 |
| Background Strain: | 129 |
| Culturing Protocol: | Std_mESC_Culture.doc |
| Description: | This mouse ES cell line contains a loxed cassette acceptor (LCA) allele that may be utilized for the exchange of DNAs of interest into the Sox17 gene locus by recombinase mediated cassette exchange (RMCE). A 3.79 kb region of the gene (exons 3-5) was replaced by tandemly oriented lox66 and lox2272 sites as well as both positive and negative selectable markers. |
Genetic Alterations
| 1) Targeted Mutagenesis | |
|---|---|
| Type of Allele | Cassette Acceptor |
| Targeted Gene | SRY-box containing gene 17 (Sox17 - NCBI GeneID:20671) |
| Targeted Allele |
targeted mutation 1
(Sox17tm1(LCA) - MGI:107543)
|
| Description of Targeting Vector | pSox17.LCA e targeting vector contains 10.288 kb 5’ arm and 4.525 kb5 3’arm. Lox66 and Lox2272 sites are inserted flanking PuTK selection marker for positive selection for targeting events with puromycin and negative selection for RMCE events with ganciclovir. |
| Targeting Vector Genbank File |
pmSox17.LCA.gb |
| Citations | Not Available |
Associated Images
| Image 1 | |
|---|---|
|
Description: A 3.793 kb region of the mouse Sox17 gene was replaced with Lox66 site, a puromycin-delta-thymidine kinase fusion gene driven by the mouse phosphoglycerol kinase promoter, a neomycin resistant gene driven by the bacterial EM7 promoter (EM7neo), and a lox2272 site by homologous recombination in ES cells. Reference: Not provided |
Repositories
| Magnuson Lab | |
|---|---|
 Request this resource
|
Stock #: Not provided Availability Notes: Not provided |
Contact Information
| Preferred Contact | |
|---|---|
| Name | Mark Magnuson |
| Institution | Vanderbilt University |
| Phone | 615-322-7006 |
| mark.magnuson@vanderbilt.edu | |
| Primary Lab Contact | |
| Name | Susan Hipkens |
| Institution | Vanderbilt University |
| Phone | 615-343-7422 |
| susan.hipkens@vanderbilt.edu | |
Associated Publications
No publications associated
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