Rosa26LCA - ES Cell Line RES262
ESC Line Information
| Cell Line Name: | Rosa26LCA |
|---|---|
| Parental Cell Line: | TL-1 |
| Background Strain: | 129 |
| Culturing Protocol: | Std_mESC_Culture.doc |
| Description: | This ES cell line contains a loxed cassette acceptor (LCA) allele in which a 5.17 kb region of the gene has been replaced by a Lox66 site, a puromycin-(delta)-thymidine kinase fusion gene driven by the mouse phosphoglycerol kinase promoter, a kanamycin resistance gene driven by the bacterial EM7 promoter, and a Lox2272 site. These features enable use of Recombinase-Mediated Cassette Exchange (RMCE) for the rapid insertion of various DNAs into the the Rosa26 gene locus. |
Genetic Alterations
| 1) Targeted Mutagenesis | |||||
|---|---|---|---|---|---|
| Type of Allele | Cassette Acceptor | ||||
| Targeted Gene | gene trap ROSA 26, Philippe Soriano (Gt(ROSA)26Sor - NCBI GeneID:14910) | ||||
| Targeted Allele |
targeted mutation 1
(Rosa26tm1(LCA) - MGI:104735)
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| Description of Targeting Vector | The Rosa 26 cassette acceptor allele was created by replacing a 5.165 kb region of this gene containing exon 1 with a floxed tk-neo cassette, a puromycin-delta-thymidine kinase fusion gene driven by the mouse phosphoglycerol kinase promoter (pU-deltaTK) and a neomycin resistant gene driven by the bacterial EM7 promoter (EM7neo) flanked by minimal (34 bp) tandemly oriented lox71 and lox2272 sites. |
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| Targeting Vector Genbank File |
pRosa26.LCA.gb |
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| Citations |
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Associated Images
| Image 1 | |
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Description: Homologous recombination in ES cells was performed to generate a loxed cassette acceptor allele. This ES cell line contains a loxed cassette acceptor (LCA) allele in which a 5.17 kb region of the gene has been replaced by a lox71 site, a puromycin-(delta)-thymidine kinase fusion gene driven by the mouse phosphoglycerol kinase promoter, a kanamycin resistance gene driven by the bacterial EM7 promoter, and a lox2272 site. These features enable use of Recombinase-Mediated Cassette Exchange (RMCE) for the rapid insertion of various DNAs into the the Rosa26 gene locus. Reference: Chen SX, Osipovich AB, et al. PMID: 21324933 |
Repositories
| Magnuson Lab | |
|---|---|
 Request this resource
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Stock #: Not provided Availability Notes: Not provided |
Contact Information
| Preferred Contact | |
|---|---|
| Name | Mark Magnuson |
| Institution | Vanderbilt University |
| Phone | 615-322-7006 |
| mark.magnuson@vanderbilt.edu | |
| Primary Lab Contact | |
| Name | Susan Hipkens |
| Institution | Vanderbilt University |
| Phone | 615-343-7422 |
| susan.hipkens@vanderbilt.edu | |
Associated Publications
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