Rosa26EN-CFP-bGpA-Neo - ES Cell Line RES2562
ESC Line Information
| Cell Line Name: | Rosa26EN-CFP-bGpA-Neo |
|---|---|
| Parental Cell Line: | TL1 Rosa26{LCA} clone 5B9 |
| Background Strain: | 129 |
| Culturing Protocol: | std_mesc_culture.doc |
| Description: | This ES cell line contains CFP (Cerulean) inserted into a Rosa26LCA allele by recombinase mediated cassette exchange. These cells were used to identify an optimal combination of regulatory elements for fluorescent protein expression from single a genomic copy. |
Genetic Alterations
| 1) RMCE Targeted Mutagenesis | |||||
|---|---|---|---|---|---|
| Type of Allele | Cassette Acceptor | ||||
| Targeted Gene | gene trap ROSA 26, Philippe Soriano (Gt(ROSA)26Sor - NCBI GeneID:14910) | ||||
| Targeted Allele |
targeted mutation 1
(Rosa26tm1(LCA) - MGI:104735)
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| Description of Targeting Vector | The Rosa 26 cassette acceptor allele was created by replacing a 5.165 kb region of this gene containing exon 1 with a floxed tk-neo cassette, a puromycin-delta-thymidine kinase fusion gene driven by the mouse phosphoglycerol kinase promoter (pU-deltaTK) and a neomycin resistant gene driven by the bacterial EM7 promoter (EM7neo) flanked by minimal (34 bp) tandemly oriented lox71 and lox2272 sites. |
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| Targeting Vector Genbank File |
pRosa26.LCA.gb |
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| Recombinase-Mediated Cassette Exchange Stage | |||||
| Type of Allele: | Gene Replacement | ||||
| Exchanged Cassette Gene | Cerulean Fluorescent Protein (CFP) | ||||
| Exchanged Cassette Allele Name | Rosa26CFP | ||||
| Description of Exchange Vector | not available |
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| Exchange Vector Genbank File: | pl451.en.cfp.bgbpa.neo.gb | ||||
| Citations |
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Associated Images
| Image 1 | |
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Description: A cyan (Cerulean) fluorescent protein gene was placed under the control of the Rosa 26 gene locus. The exchange plasmid also contains a 51 bp translational enhancer (5' leader sequence from Xenopus beta-globin gene), a Kozak sequence upstream of the start codon and a polyA site from rabbit beta globin gene. Reference: PMID: 21324933 |
Repositories
| MMRRC | |
|---|---|
 Request via www.mmrrc.org website
|
Stock #: VUMC Availability Notes: Not provided |
Contact Information
| Preferred Contact | |
|---|---|
| Name | Mark Magnuson |
| Institution | Vanderbilt University |
| Phone | 615-322-7006 |
| mark.magnuson@vanderbilt.edu | |
| Primary Lab Contact | |
| Name | Susan Hipkens |
| Institution | Vanderbilt University |
| Phone | 615-343-7422 |
| susan.hipkens@vanderbilt.edu | |
Associated Publications
No publications associated
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