Insm1^LCA - ES Cell Line RES256

ESC Line Information

Cell Line Name:	Insm1^LCA
Parental Cell Line:	TL-1
Background Strain:	129
Culturing Protocol:	Std_mESC_Culture.doc
Description:	This ES cell line contains a loxed cassette acceptor (LCA) allele that may be utilized for the exchange of DNAs of interest into the Insm1 gene locus by recombinase mediated cassette exchange (RMCE). This LCA uses a lox 66/71 and lox 2272 strategy for RMCE and allows a ~5.2 kb region of this single exon gene to be manipulated. This enables a variety of differnt types of experiments to be performed using a range of cassette designs.

Genetic Alterations

1) Targeted Mutagenesis
Type of Allele	Cassette Acceptor
Targeted Gene	insulinoma-associated 1 (Insm1 - NCBI GeneID:53626)
Targeted Allele	targeted mutation 1 (Insm1^tm1(LCA) - MGI:1859980)
Description of Targeting Vector	This LCA uses a lox 66/71 and lox 2272 strategy for RMCE and allows a ~5.2 kb region of this single exon gene to be manipulated. This enables a variety of differnt types of experiments to be performed using a range of cassette designs.
Targeting Vector Genbank File	Insm1.GT.gb
Citations	Not Available

Associated Images

Image 1
	Description: Through homologous recombination in ES cells, a 5232 bp fragment containing the proximal promoter, exon and the 3' flanking region of Insm1 was deleted and replaced with a positive-negative selection cassette. The selection cassette consists of a pgk-pu(Delta)tk cassette for positive negative selection in ES cells, and an EM7-kanomycin cassette for positive selection in bacterial cells. Reference: Not provided

Image 1

Description:
Through homologous recombination in ES cells, a 5232 bp fragment containing the proximal promoter, exon and the 3' flanking region of Insm1 was deleted and replaced with a positive-negative selection cassette. The selection cassette consists of a pgk-pu(Delta)tk cassette for positive negative selection in ES cells, and an EM7-kanomycin cassette for positive selection in bacterial cells.

Reference:
Not provided

Repositories

Magnuson Lab
Request this resource	Stock #: Not provided Availability Notes: Not provided

Contact Information

Preferred Contact
Name	Mark Magnuson
Institution	Vanderbilt University
Phone	615-322-7006
Email	mark.magnuson@vanderbilt.edu
Primary Lab Contact
Name	Susan Hipkens
Institution	Vanderbilt University
Phone	615-343-7422
Email	susan.hipkens@vanderbilt.edu