GckLCA - ES Cell Line RES255
ESC Line Information
| Cell Line Name: | GckLCA |
|---|---|
| Parental Cell Line: | TL-1 |
| Background Strain: | 129 |
| Culturing Protocol: | Std_mESC_Culture.doc |
| Description: | This ES cell line contains a loxed cassette acceptor (LCA) allele that may be utilized for the exchange of DNAs of interest into the glucokinase locus by recombinase mediated cassette exchange (RMCE). This LCA uses a loxP/inverted loxP strategy for RMCE and allows sequences between - 6 kb and + 5 kb (relevative to the hepatic-specific promoter) to be manipulated. This enables a variety of different types of experiments to be performed using a range of cassette designs.
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Genetic Alterations
| 1) Targeted Mutagenesis | |||||
|---|---|---|---|---|---|
| Type of Allele | Cassette Acceptor | ||||
| Targeted Gene | glucokinase (Gck - NCBI GeneID:19213) | ||||
| Targeted Allele |
targeted mutation 4
(gktm4(LCA) - MGI:1270854)
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| Description of Targeting Vector |
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| Targeting Vector Genbank File |
GK.KI.DT.gb |
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| Citations |
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Associated Images
| Image 1 | |
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Description: Through homologous recombination in ES cells, an 11.1 kb region of the gck gene spanning from ~6 kb upstream of hepatic exon 1 to ~0.4 kb downstream of exon 3 was removed and replaced with a neo/tk selection cassette flanked by inversely oriented loxP sites. Reference: Not provided |
Repositories
| Magnuson Lab | |
|---|---|
 Request this resource
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Stock #: Not provided Availability Notes: Not provided |
Contact Information
| Preferred Contact | |
|---|---|
| Name | Mark Magnuson |
| Institution | Vanderbilt University |
| Phone | 615-322-7006 |
| mark.magnuson@vanderbilt.edu | |
| Primary Lab Contact | |
| Name | Susan Hipkens |
| Institution | Vanderbilt University |
| Phone | 615-343-7422 |
| susan.hipkens@vanderbilt.edu | |
Associated Publications
No publications associated
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