Ptf1aLCA - ES Cell Line RES253
ESC Line Information
| Cell Line Name: | Ptf1aLCA |
|---|---|
| Parental Cell Line: | TL-1 |
| Background Strain: | 129 |
| Culturing Protocol: | Std_mESC_Culture.doc |
| Description: | This ES cell line contains a loxed cassette acceptor (LCA) allele in which a 4.1 kb region of this gene (including the proximal promoter and both exons 1 and 2) was replaced with a loxP site, an inverted loxP site, and both positive and negative selectable markers. This enables the use of Recombinase-Mediated Cassette Exchange (RMCE) to easily insert various reporter genes or to make other modifications of the Ptf1a gene locus. |
Genetic Alterations
| 1) Targeted Mutagenesis | |
|---|---|
| Type of Allele | Cassette Acceptor |
| Targeted Gene | pancreas specific transcription factor, 1a (Ptf1a - NCBI GeneID:19213) |
| Targeted Allele |
targeted mutation 1
(Ptf1atm1(LCA) - MGI:1328312)
|
| Description of Targeting Vector | Not provided |
| Targeting Vector Genbank File |
Ptf1a.LCA.gb |
| Citations | Not Available |
Associated Images
| Image 1 | |
|---|---|
|
Description: Through homologous recombination in ES cells, a 4.1 kb Sal I-Sal I fragment containing the proximal promoter, exon 1 and 2, and the 3' flanking region of Ptf1a gene was deleted and replaced with a positive-negative selection cassette. The selection cassette consists of pgk-neo, pgk-tk flanked by inverted loxP sites. Reference: Not provided |
Repositories
| Magnuson Lab | |
|---|---|
 Request this resource
|
Stock #: Not provided Availability Notes: Not provided |
Contact Information
| Preferred Contact | |
|---|---|
| Name | Mark Magnuson |
| Institution | Vanderbilt University |
| Phone | 615-322-7006 |
| mark.magnuson@vanderbilt.edu | |
| Primary Lab Contact | |
| Name | Susan Hipkens |
| Institution | Vanderbilt University |
| Phone | 615-343-7422 |
| susan.hipkens@vanderbilt.edu | |
Associated Publications
| Publication | Citation |
|---|---|
| 18294628 | Burlison JS, Long Q, Fujitani Y, Wright CV, Magnuson MA Pdx-1 and Ptf1a concurrently determine fate specification of pancreatic multipotent progenitor cells. (2008) Dev Biol 316: 74-86 (Added December 20, 2010) |
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