Pdx1deltaII - Mouse Strain RES242
Mouse Information
| Common Name: | Pdx1deltaII |
|---|---|
| MGI Official Name: | Pdx1tm6Cvw |
| Description: | Targeted deletion of Area II 5' cis-regulatory region of Pdx1 |
| Categories: | None specified. |
Genetic Alterations
| 1) RMCE Targeted Mutagenesis | |
|---|---|
| Type of Allele | Cassette Acceptor |
| Targeted Gene | pancreatic and duodenal homeobox 1 (Pdx1 - NCBI GeneID:18609) |
| Targeted Allele |
targeted mutation 1
(Pdx1tm1(LCA) - MGI:102851)
|
| Description of Targeting Vector | An 8.62 kb region of this gene has been replaced by tandemly oriented Lox66 and Lox2272 sites flanking positive (puromycin) and negative (HSV-TK) selectable markers. |
| Targeting Vector Genbank File |
ppdx1tv.gb |
| Recombinase-Mediated Cassette Exchange Stage | |
| Type of Allele: | Gene Replacement |
| Exchanged Cassette Gene | Not provided. |
| Exchanged Cassette Allele Name | Pdx1AIId |
| Description of Exchange Vector | The pdx1 Area II enhancer region was deleted from the pPdx1.Ex1 basal exchange vector (255 bp from -2168/-1913 relative to the primary transcription start site reported by Gerrish, K.et.al., JBC 2000). This will replace endogenous pdx1 sequences, and allow for assessment of the role of this enhancer region in pdx1 expression in vivo. Sequences flanking Area II deletion: ctcatcctgt/gaaggaaggt |
| Exchange Vector Genbank File: | pdx1AIId.gb |
| Citations | Not Available |
Strain Information
| Strain Type: | Mixed |
|---|---|
| Chimera/Founder Genetic Background: | 129S6/SvEvTac |
| Current Genetic Background: | Not provided (date recorded: Not provided) |
| Strain Description: | Not provided |
Associated Images
| Image 1 | |
|---|---|
|
Description: The pdx1 Area II enhancer region was deleted from the pPdx1.Ex1 basal exchange vector (255 bp from -2168/-1913 relative to the primary transcription start site reported by Gerrish, K.et.al., JBC 2000). This will replace endogenous pdx1 sequences, and allow for assessment of the role of this enhancer region in pdx1 expression in vivo. Sequences flanking Area II deletion: ctcatcctgt/gaaggaaggt Reference: Not provided |
Repositories
| Wright Lab | |
|---|---|
 Request this resource
|
Stock #: Not provided Availability Notes: Not provided |
Contact Information
| Preferred Contact | |
|---|---|
| Name | Chris Wright |
| Institution | Vanderbilt University |
| Phone | 615-343-8256 |
| chris.wright@vanderbilt.edu | |
| Primary Lab Contact | |
| Name | Yu-Ping Yang |
| Institution | Vanderbilt University Medical Center |
| Phone | 615-343-8258 |
| yuping.yang@vanderbilt.edu | |
Associated Publications
No publications associated
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