Rosa26^{Non-EN-CFP-Neo} - ES Cell Line RES2401

ESC Line Information

Cell Line Name:	Rosa26^{Non-EN-CFP-Neo}
Parental Cell Line:	TL1 Rosa26{LCA} clone 5B9
Background Strain:	129
Culturing Protocol:	std_mesc_culture.doc
Description:	This ES cell line contains CFP (Cerulean) inserted into a Rosa26^LCA allele by recombinase mediated cassette exchange. These cells were used to identify the optimal arrangement of regulatory elements for fluorescent protein expression from a single genomic copy.

Genetic Alterations

1) RMCE Targeted Mutagenesis

Type of Allele

Gene Replacement

Targeted Gene

gene trap ROSA 26, Philippe Soriano (Gt(ROSA)26Sor - NCBI GeneID:14910)

Targeted Allele

targeteted mutation 1.8 (Rosa26^{tm1.8(Non-EN-CFP)})

Description of Targeting Vector

Not available

Targeting Vector Genbank File

pRosa26.LCA3.gb

Recombinase-Mediated Cassette Exchange Stage

Type of Allele:

Not available

Exchanged Cassette Gene

Not provided. (MGI:14910)

Exchanged Cassette Allele Name

Rosa26^LCA

Description of Exchange Vector

Not provided

Exchange Vector Genbank File:

prosa.nonen.cfp.bgsplicepa.neo.gb

Citations

PubMedID	Citation
21324933	Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice. (2011) Dis Model Mech 4: 537-47 (Added 2012-09-24 16:36:23.369844)

Associated Images

Image 1
	Description: A cyan (Cerulean) fluorescent protein gene was placed under the control of a 4kb Rosa 26 promoter element followed by an intronic region and polyA site from the rabbit beta globin gene. Reference: Chen SX, Osipovich AB, et al. PMID: 21324933

Image 1

Description:

A cyan (Cerulean) fluorescent protein gene was placed under the control of a 4kb Rosa 26 promoter element followed by an intronic region and polyA site from the rabbit beta globin gene.

Reference:

Chen SX, Osipovich AB, et al. PMID: 21324933

Repositories

MMRRC
Request via www.mmrrc.org website	Stock #: VUMC Availability Notes: Not provided

Contact Information

Preferred Contact
Name	Mark Magnuson
Institution	Vanderbilt University
Phone	615-322-7006
Email	mark.magnuson@vanderbilt.edu
Primary Lab Contact
Name	Susan Hipkens
Institution	Vanderbilt University
Phone	615-343-7422
Email	susan.hipkens@vanderbilt.edu