Rosa26EN-Cherry-Neo - ES Cell Line RES2281
ESC Line Information
| Cell Line Name: | Rosa26EN-Cherry-Neo |
|---|---|
| Parental Cell Line: | TL1-Rosa26{LCA} 5B9 |
| Background Strain: | 129 |
| Culturing Protocol: | std_mesc_culture.doc |
| Description: | This ES cell line expresses mCherry, a red fluorescent protein, under control of the endogenous Rosa26 gene. A cassette containing mCherry and other sequences to assure efficient expression were inserted into the Rosa26[LCA] allele by recombinase mediated cassette exchange. These cells will be used to identify an optimal combination of regulatory elements for fluorescent protein expression from a single gene copy and as a reference cell line for fluorescent cell sorting. |
Genetic Alterations
| 1) RMCE Targeted Mutagenesis | |
|---|---|
| Type of Allele | Cassette Acceptor |
| Targeted Gene | gene trap ROSA 26, Philippe Soriano (Gt(ROSA)26Sor - NCBI GeneID:14910) |
| Targeted Allele |
targeted mutation 1
(Rosa26tm1(LCA) - MGI:104735)
|
| Description of Targeting Vector | Not available |
| Targeting Vector Genbank File |
pRosa26.LCA.gb |
| Recombinase-Mediated Cassette Exchange Stage | |
| Type of Allele: | Gene Replacement |
| Exchanged Cassette Gene | Not provided. (mCherry) |
| Exchanged Cassette Allele Name | Rosa26EN-Cherry-Neo |
| Description of Exchange Vector | not available |
| Exchange Vector Genbank File: | prosa.en.cherry.bgsplicepa.neo_1.gb |
| Citations | Not Available |
Associated Images
| Image 1 | |
|---|---|
|
Description: A red (mCherry) fluorescent protein gene was placed under control of a 4 kb Rosa 26 promoter element. The exchange plasmid also contains a 51 bp translational enhancer (5' leader sequence from Xenopus beta-globin gene), a Kozak sequence upstream of the start codon, which is followed by an intronic region and polyA site from the rabbit beta-globin gene. Reference: Not provided |
Repositories
| MMRRC | |
|---|---|
 Request via www.mmrrc.org website
|
Stock #: VUMC Availability Notes: Not provided |
Contact Information
| Preferred Contact | |
|---|---|
| Name | Mark Magnuson |
| Institution | Vanderbilt University |
| Phone | 615-322-7006 |
| mark.magnuson@vanderbilt.edu | |
| Primary Lab Contact | |
| Name | Susan Hipkens |
| Institution | Vanderbilt University |
| Phone | 615-343-7422 |
| susan.hipkens@vanderbilt.edu | |
Associated Publications
No publications associated
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