NOD-Rag-Perforin Akita - Mouse Strain RES211

Mouse Information

Common Name:	NOD-Rag-Perforin Akita
MGI Official Name:	NOD.Cg-Rag1^tm1Mom Ins2^Akita Prf1^tm1Sdz/Sz
Description:	The NOD-Rag1^null Prf1^null Ins2^Akita mouse is the first immunodeficient, spontaneously hyperglycemic mouse strain described that is based on the Ins2^Akita mutation. This strain is suitable as hosts for human islet and human beta stem and progenitor cell transplantation in the absence of the need for pharmacological induction of diabetes. This strain of mice also has low levels of innate immunity and can be engrafted with a human immune system for the study of human islet allograft rejection.
Categories:	HUMANE

Genetic Alterations

1) Targeted Mutagenesis

Type of Allele

Global Null

Targeted Gene

recombination activating gene 1 (Rag1 - NCBI GeneID:19373)

Targeted Allele

Not provided (MGI:97848)

Description of Targeting Vector

Not provided

Targeting Vector Genbank File

Not provided

Citations

PubMedID	Citation
18785974	Non-obese diabetic-recombination activating gene-1 (NOD-Rag1 null) interleukin (IL)-2 receptor common gamma chain (IL2r gamma null) null mice: a radioresistant model for human lymphohaematopoietic engraftment. (2008) Clin Exp Immunol 154: 270-84 (Added 2009-04-21 09:46:49)

2) Targeted Mutagenesis

Type of Allele

Global Null

Targeted Gene

perforin 1 (pore forming protein) (Prf1 - NCBI GeneID:18646)

Targeted Allele

Not provided (Prf1^tm1sdz - MGI:97551)

Description of Targeting Vector

Not provided

Targeting Vector Genbank File

Not provided

Citations

PubMedID	Citation
18563383	A new immunodeficient hyperglycaemic mouse model based on the Ins2Akita mutation for analyses of human islet and beta stem and progenitor cell function. (2008) Diabetologia 51: 1449-56 (Added 2009-04-20 17:03:25)

Strain Information

Strain Type:	Congenic Strain
Chimera/Founder Genetic Background:	Not provided
Current Genetic Background:	Not provided (date recorded: Not provided)
Strain Description:	Not provided

Associated Images

Image 1
	Description: Spontaneous development of hyperglycemia in NOD-Rag1nullPrf1nullINs2+/Akita mice. Groups of mice werer analysed for the development of hyperglycemia over time and strtified by sex. Additionally, littermates of each sex were typed at Ins2 to determine mice that were homozygous wild-type (white circles) or harboured a single Akita allele (black circles). (hyperglycemia) Reference: PMID:18563383 Peason et al. (2008) Diabetologia 51:1449-1456; Fig.1 with kind permission of Springer Science and Business Media
Image 2
	Description: Reversal of diabetes in NOD-Rag1null Prf1null Ins2+/Akita mice following human islet transplantation. Hyperglycemic male mice were randomised into three groups and receiced either 2,000 (a, n=6), 3,000 (b, n=3), or 4,000 (c, n=4) IEQ of human islets into the renal subcapsular space. Blood glucose levels in induvidual mice at each human islet dose are shown over the follow-up period.(islet transplantation) Reference: PMID:18563383 Peason et al. (2008) Diabetologia 51:1449-1456; Fig.4 with kind permission of Springer Science and Business Media

Image 1

Description:

Spontaneous development of hyperglycemia in NOD-Rag1nullPrf1nullINs2+/Akita mice. Groups of mice werer analysed for the development of hyperglycemia over time and strtified by sex. Additionally, littermates of each sex were typed at Ins2 to determine mice that were homozygous wild-type (white circles) or harboured a single Akita allele (black circles). (hyperglycemia)

Reference:

PMID:18563383

Peason et al. (2008) Diabetologia 51:1449-1456; Fig.1

with kind permission of Springer Science and Business Media

Image 2

Description:

Reversal of diabetes in NOD-Rag1null Prf1null Ins2+/Akita mice following human islet transplantation. Hyperglycemic male mice were randomised into three groups and receiced either 2,000 (a, n=6), 3,000 (b, n=3), or 4,000 (c, n=4) IEQ of human islets into the renal subcapsular space. Blood glucose levels in induvidual mice at each human islet dose are shown over the follow-up period.(islet transplantation)

Reference:

PMID:18563383

Peason et al. (2008) Diabetologia 51:1449-1456; Fig.4

with kind permission of Springer Science and Business Media