Autosomal beta-cell toxigene strain - Mouse Strain RES209

Mouse Information

Common Name:	Autosomal beta-cell toxigene strain
MGI Official Name:	NOD.Cg-Prkdc^scid Il2rg^tm1Wjl Tg(Ins2-HBEGF)^6832Ugfm/Sz
Description:	NOD-Prkdc^scid IL2rg ^null Tg(Ins2- HBEGF)^6832Ugfm mice are deficient in mature lymphocytes and NK cells, survive beyond 16 months of age, and even after sublethal irradiation resist lymphoma development. They can be induced to become hypoglycemic when Diphtheria toxin is used. 100% of beta cells will be depleted upon administration of diphtheria toxin.
Categories:	HUMANE

Genetic Alterations

1) BAC or Transgene Insertion

Type of Vector

Plasmid

Promoter

Rat insulin II (RIP)

Expressed Gene

Diphtheria toxin receptor (DTR)

Description of Transgene

The RIP-DTR transgene was made by Pedro Herrera by subcloning the human HB-EGF cDNA into the EcoRI/SaII sites of a plasmid containing a 0.7Kb-long fragment of the rat insulin II promoter, and a 1.6 kb-long sequence containing an intron and the polyA signal of the rabbit beta globin gene.

Vector Genbank File

Not provided

Citations

Not provided

2) Targeted Mutagenesis

Type of Allele

Global Null

Targeted Gene

interleukin 2 receptor, gamma chain (Il2rg - NCBI GeneID:16186)

Targeted Allele

Not provided (Il2rg^tm1Wjl - MGI:96551)

Description of Targeting Vector

Not provided

Targeting Vector Genbank File

Not provided

Citations

PubMedID	Citation
18096436	A new Hu-PBL model for the study of human islet alloreactivity based on NOD-scid mice bearing a targeted mutation in the IL-2 receptor gamma chain gene. (2008) Clin Immunol 126: 303-14 (Added 2011-03-02 10:32:43.247619)

Strain Information

Strain Type:	Congenic Strain
Chimera/Founder Genetic Background:	Not provided
Current Genetic Background:	Not provided (date recorded: Not provided)
Strain Description:	Not provided