Rosa26R26-228-IBRE4-TA-Cerulean - ES Cell Line RES2002
ESC Line Information
| Cell Line Name: | Rosa26R26-228-IBRE4-TA-Cerulean |
|---|---|
| Parental Cell Line: | Rosa26{LCA} clone 5B9 |
| Background Strain: | 129 |
| Culturing Protocol: | std_mesc_culture.doc |
| Description: | In these cells Rosa26 gene sequences from -228 to +81 were replaced by a BMP response element (IBRE4) fused to a TATA-Cerulean (CFP) reporter. These ESCs may be useful for assess BMP responsiveness. |
Genetic Alterations
| 1) RMCE Targeted Mutagenesis | |
|---|---|
| Type of Allele | Cassette Acceptor |
| Targeted Gene | gene trap ROSA 26, Philippe Soriano (Gt(ROSA)26Sor - NCBI GeneID:14910) |
| Targeted Allele |
targeted mutation 1
(Rosa26tm1(LCA) - MGI:104735)
|
| Description of Targeting Vector | Not available |
| Targeting Vector Genbank File |
pRosa26.LCA.gb |
| Recombinase-Mediated Cassette Exchange Stage | |
| Type of Allele: | Gene Replacement |
| Exchanged Cassette Gene | Not provided. (R26-228-IBRE4-TA-Cerulean) |
| Exchanged Cassette Allele Name | Rosa26R26-228-IBRE4-TA-Cerulean |
| Description of Exchange Vector | not available |
| Exchange Vector Genbank File: | pr26228ibre4tacerulean.gb |
| Citations | Not Available |
Associated Images
| No associated images have been supplied | |
Repositories
| MMRRC | |
|---|---|
 Request via www.mmrrc.org website
|
Stock #: Not provided Availability Notes: Not provided |
Contact Information
| Preferred Contact | |
|---|---|
| Name | Palle Serup |
| Institution | Hagedorn Research Institute |
| Phone | +45 4443 9822 |
| pas@novonordisk.com | |
| Primary Lab Contact | |
| Name | Tino Klein |
| Institution | Not provided |
| Phone | Not provided |
| tkle@hagedorn.dk | |
Associated Publications
No publications associated
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Resource Tags
embryonic, es, esc, Rosa26LCA clone 5B9, Rosa26R26-228-IBRE4-TA-Cerulean, Sassy CBP, stem
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