pcklox - Mouse Strain RES194
|MGI Official Name:||pcktm1.1Mgn|
These mice may be used to study tissue-specific functions of phosphoenolpyruvate carboxykinase (Pck1). This enzyme is essential for gluconeogenesis and also is important for regulating anapleurosis/catapleurosis of TCA cycle intermediates. By generating mice that are homozygous for the pcklox allele and that contain a tissue-specific Cre transgene Pck1 can be deleted from various sites.
Cre-lox floxed alleles
|1) Targeted Mutagenesis|
|Type of Allele||Conditional Null|
|Targeted Gene||phosphoenolpyruvate carboxykinase 1 (Pck1 - NCBI GeneID:18534)|
targeted mutation 1.1, Mark A Magnuson
(Pck1tm1.1Mgn - MGI:2449283)
|Description of Targeting Vector||
A gene targeting strategy was used to flank exons 4 and 5 in the Pck1 gene with two tandomly-oriented loxP sites. DNA PCR utilizing primers 5'-AATGTTCTCTGCAAGTCCTGGTG-3' and 5'-TCTGTCAGTTCAATACCAATCT-3' amplify a 616 bp pckloxband and a 518 bp wild type band. Homozygous Pcklox/lox animals are viable. Pck1 activity and protein content in liver and kidney do not differ from wild type. Heterozygous animals are also viable and do not differ from the wild type.
|Targeting Vector Genbank File||
|Strain Type:||Congenic Strain|
|Chimera/Founder Genetic Background:||129S6/SvEvTac|
|Current Genetic Background:||99.76% C57BL/6 (date recorded: Not provided)|
Mice carrying the pcklox allele have been backcrossed ten times into a C57Bl/6J background.
|Request this resource||
Stock #: VUMC - AX
Availability Notes: Not currently maintained as live mice.
|Request via www.mmrrc.org website||
Stock #: 011950-UNC
Availability Notes: Not provided
|10938127||She P, Shiota M, Shelton KD, Chalkley R, Postic C, Magnuson MA Phosphoenolpyruvate carboxykinase is necessary for the integration of hepatic energy metabolism. (2000) Mol Cell Biol 20: 6508-17 (Added January 31, 2013)|
There are no comments for this entry.Login to add comments
Request this Resource
Resource History & Actions
Last modified on Aug 16, 2010
Login to edit or request an edit