pcklox - Mouse Strain RES194
Mouse Information
| Common Name: | pcklox |
|---|---|
| MGI Official Name: | pcktm1.1Mgn |
| Description: | These mice may be used to study tissue-specific functions of phosphoenolpyruvate carboxykinase (Pck1). This enzyme is essential for gluconeogenesis and also is important for regulating anapleurosis/catapleurosis of TCA cycle intermediates. By generating mice that are homozygous for the pcklox allele and that contain a tissue-specific Cre transgene Pck1 can be deleted from various sites. |
| Categories: |
Cre-lox floxed alleles |
Genetic Alterations
| 1) Targeted Mutagenesis | |||||
|---|---|---|---|---|---|
| Type of Allele | Conditional Null | ||||
| Targeted Gene | phosphoenolpyruvate carboxykinase 1 (Pck1 - NCBI GeneID:18534) | ||||
| Targeted Allele |
targeted mutation 1.1, Mark A Magnuson
(Pck1tm1.1Mgn - MGI:2449283)
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| Description of Targeting Vector | A gene targeting strategy was used to flank exons 4 and 5 in the Pck1 gene with two tandomly-oriented loxP sites. DNA PCR utilizing primers 5'-AATGTTCTCTGCAAGTCCTGGTG-3' and 5'-TCTGTCAGTTCAATACCAATCT-3' amplify a 616 bp pckloxband and a 518 bp wild type band. Homozygous Pcklox/lox animals are viable. Pck1 activity and protein content in liver and kidney do not differ from wild type. Heterozygous animals are also viable and do not differ from the wild type. |
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| Targeting Vector Genbank File |
pmPEPCK.KO2.gb |
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| Citations |
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Strain Information
| Strain Type: | Congenic Strain |
|---|---|
| Chimera/Founder Genetic Background: | 129S6/SvEvTac |
| Current Genetic Background: | 99.76% C57BL/6 (date recorded: Not provided) |
| Strain Description: | Mice carrying the pcklox allele have been backcrossed ten times into a C57Bl/6J background. |
Associated Images
| Image 1 | |
|---|---|
|
Description: The figure shows how several different pck alleles were generated through a combination of gene targeting and Cre-mediated recombination. (A) Top, map of the pckw allele. Exons are indicated as solid rectangles. Middle, map of the PEPCK gene targeting vector. The vector contains a pgk-neo cassette, a pgk-tk cassette, and three loxP sites (triangles). Two of the loxP sites flank neo, and the third is located between exons 4 and 5 in the PEPCK gene. The pcklox+neo allele was generated by homologous recombination (HR) in ES cells. Bottom, the pcklox and pckdel alleles were derived from pcklox+neo allele by partial and total Cre-mediated recombination, respectively. Reference: 10938127 |
Repositories
| Magnuson Lab | |
|---|---|
 Request this resource
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Stock #: VUMC - AX Availability Notes: Not currently maintained as live mice. |
| MMRRC | |
|---|---|
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Request via www.mmrrc.org website
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Stock #: 011950-UNC Availability Notes: Not provided |
Contact Information
| Preferred Contact | |
|---|---|
| Name | Jody Peters |
| Institution | Vanderbilt University |
| Phone | 615-343-7422 |
| jody.peters@vanderbilt.edu | |
Associated Publications
| Publication | Citation |
|---|---|
| 10938127 | She P, Shiota M, Shelton KD, Chalkley R, Postic C, Magnuson MA Phosphoenolpyruvate carboxykinase is necessary for the integration of hepatic energy metabolism. (2000) Mol Cell Biol 20: 6508-17 (Added January 31, 2013) |
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