gkK414E - Mouse Strain RES192
Mouse Information
| Common Name: | gkK414E |
|---|---|
| MGI Official Name: | Gcktm2Mgn |
| Description: | This line of mice contains a gkK414E point mutation that was introduced by gene knock-in. This point mutation was identified in a MODY-GK pedigree. These mice will be useful for studies of sustained hyperglycemia since they contain only a single mutation and are congenic with the C57BL/6J strain. |
| Categories: | None specified. |
Genetic Alterations
| 1) Targeted Mutagenesis | |||||
|---|---|---|---|---|---|
| Type of Allele | Global Mutation | ||||
| Targeted Gene | Glucokinase (Gck - NCBI GeneID:103988) | ||||
| Targeted Allele |
targeted mutation 2
(Gcktm2Mgn - MGI:3701697)
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| Description of Targeting Vector | A single base pair mutation was introduced into exon 9 via site specific mutagenesis to change amino acid 414 from lysine to glutamic acid. Genotype by DNA PCR using primers 5'-TGT CTC AAT TTG CTG TGT CCT CCA-3' and 5'-ATG TGT GAG TGT GCC AAT ATG AGT-3'. These primers will amplify a 636 bp fragment from the wild type allele and a 741 bp fragment from the targeted (mutant) allele. These animals are homozygous lethal. Heterozygous mice are viable. They are hyperglycemic and hypoinsulinemic when compared to wild type. |
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| Targeting Vector Genbank File |
pBOB2.K414E.gb |
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| Citations |
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Strain Information
| Strain Type: | Congenic Strain |
|---|---|
| Chimera/Founder Genetic Background: | 129S6/SvEvTac |
| Current Genetic Background: | C57BL/6J (date recorded: Not provided) |
| Strain Description: | After achieving germline transmission mice carrying the gkK414E allele were backcrossed for eleven generations into a C57BL/6J strain. |
Associated Images
| Image 1 | |
|---|---|
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Description: Gene targeting strategy used to generate point mutations in the gk gene. Uppermost map is a diagram of the mouse gk gene showing locations of exons 3 to 10 (indicated by open boxes). The locations of the DNA fragments used as 5' and 3' hybridization probes are shown. EI, EcoRI; EV, EcoRV. Second map from top is of the gene targeting vector carrying either the K414E mutation in exon 9 depicted by the red circle or the A456V mutation in exon 10 as indicated by the blue circle. A neomycin resistance cassette (pgk-neoR), which is flanked with two loxP sites depicted by red triangles, and a HSV-thymidine kinase cassette (pgk-HSV-TK), were used for positive and negative selection, respectively. Third map from top is of the recombinant gk allele after homologous recombination (HR) carrying a floxed pgk-neoR cassette and the respective point mutation in exon 9 or 10. Fourth map from top is of the mutant gk allele after Cre recombination. Reference: M.F. Pino, K-A. Kim, K.D. Shelton, J. Lindner, S. Odili, C. Li, H.W. Collins, M. Shiota, F.M. Matschinsky and M.A. Magnuson, Glucokinase thermolability and hepatic regulatory protein binding are essential factors for predicting the blood glucose phenotype of missense mutations, Journal of Biological Chemistry, 2007 May 4, 282(18):13906-16. |
Repositories
| BCBC members may Login to request this resource. |
| MMRRC | |
|---|---|
 Request via www.mmrrc.org website
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Stock #: 015201-UCD Availability Notes: Not provided |
Contact Information
| Preferred Contact | |
|---|---|
| Name | Mark Magnuson |
| Institution | Vanderbilt University |
| Phone | 615-322-7006 |
| mark.magnuson@vanderbilt.edu | |
| Primary Lab Contact | |
| Name | Jody Peters |
| Institution | Vanderbilt University |
| Phone | 615-343-7422 |
| jody.peters@vanderbilt.edu | |
Associated Publications
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