Foxo1.DBD - Mouse Strain RES1701
Mouse Information
| Common Name: | Foxo1.DBD |
|---|---|
| MGI Official Name: | Foxo1tm1(DBD)Dac |
| Description: | This line contains a DNA binding-deficient mutant of Foxo1. The mutation may be useful for assessing the contribution of DNA co-activation in the function of Foxo1. |
| Categories: | None specified. |
Genetic Alterations
| 1) RMCE Targeted Mutagenesis | |
|---|---|
| Type of Allele | Cassette Acceptor |
| Targeted Gene | forkhead box O1 (Foxo1 - NCBI GeneID:56458) |
| Targeted Allele |
targeted mutation 1
(Foxo1tm1(LCA) - MGI:1890077)
|
| Description of Targeting Vector | Not provided |
| Targeting Vector Genbank File |
pFoxo1.LCA.gb |
| Recombinase-Mediated Cassette Exchange Stage | |
| Type of Allele: | Gene Replacement |
| Exchanged Cassette Gene | Not provided. |
| Exchanged Cassette Allele Name | Foxo1DBD |
| Description of Exchange Vector | A loxed cassette acceptor allele for Foxo1 was generated using a targeting vector that contains a lox71 site, a puromycin (delta)thymidine kinase fusion gene driven by a mouse phosphoglycerol kinase (PGK) promoter, a neomycin resistant gene driven by the bacterial EM7 promoter (EM7neo), and a lox2272 site. A 4.999 kb region of the Foxo1 gene containing exon 1 was removed in the process of gene targeting. |
| Exchange Vector Genbank File: | pFoxo1.DBD.gb |
| Citations | Not Available |
Strain Information
| Strain Type: | Mixed |
|---|---|
| Chimera/Founder Genetic Background: | 129S6/SvEvTac |
| Current Genetic Background: | 93.75% C57BL6/J (date recorded: Not provided) |
| Strain Description: | 129S6-derived chimeric males were bred to C57BL6/J females to achieve germline transmission. All subsequent matings were to C57BL6/J for a total of 4 backcrosses. |
Associated Images
| Image 1 | |
|---|---|
|
Description: Not provided Reference: Not provided |
Repositories
| Accili Lab | |
|---|---|
| No URL supplied for repository |
Stock #: Not provided Availability Notes: Not provided |
Contact Information
| Preferred Contact | |
|---|---|
| Name | Domenico Accili |
| Institution | Columbia University |
| Phone | 212-851-5332 |
| da230@columbia.edu | |
| Primary Lab Contact | |
| Name | Jody Peters |
| Institution | Vanderbilt University |
| Phone | 615-343-7422 |
| jody.peters@vanderbilt.edu | |
Associated Publications
No publications associated
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