Rosa26^{R26-228-DR5-TA-Cerulean} - Mouse Strain RES1281

Mouse Information

Common Name:	Rosa26^{R26-228-DR5-TA-Cerulean}
MGI Official Name:	Rosa26^{tm1.2(R26-228-DR5-TA-Cerulean)Mgn}
Description:	These mice were generated using RMCE to insert an exchange vector containing a modified Rosa26 promoter linked to a Cerulean fluorescent protein (CFP) reporter gene into mESCs containing a Loxed Cassette Acceptor (LCA) allele within the Rosa26 gene locus. The Rosa26 promoter in this mouse was altered by replacing DNA sequences from -228 to +81 with a multimerized retinoic acid response element (DR5) fused to a TATA box. This mouse will facilitate studies of retinoic acid signaling in an intact animal.
Categories:	Fluorescent Probes

Genetic Alterations

1) RMCE Targeted Mutagenesis
Type of Allele	Gene Replacement
Targeted Gene	gene trap ROSA 26, Philippe Soriano (Gt(ROSA)26Sor - NCBI GeneID:14910)
Targeted Allele	targeted mutation 1.2 (Rosa26{tm1.2((R26-228-DR5-Cerulean))
Description of Targeting Vector	A Rosa26 RMCE plasmid constructed for use with the ES cell loci targeted by pRosa26.LCA. In this experiment native Rosa26 gene sequence from -228 to +81 was replaced by a retinoic acid response element (DR5) fused to a TATA-Cerulean red reporter. The resulting mouse ESCs will enable the feasibility of inserting signaling sentinel cassettes into a facilitating chromosomal locus to be directly tested.
Targeting Vector Genbank File	pRosa26.LCA.gb
Recombinase-Mediated Cassette Exchange Stage
Type of Allele:	Not available
Exchanged Cassette Gene	Not provided. (MGI:14910)
Exchanged Cassette Allele Name	Rosa26^LCA
Description of Exchange Vector	Not provided
Exchange Vector Genbank File:	R26228DR5TACerulean.gb
Citations	Not Available

Strain Information

Strain Type:	Mixed
Chimera/Founder Genetic Background:	Not provided
Current Genetic Background:	129S6 and C57Bl6/J hybrid (date recorded: Not provided)
Strain Description:	129S6 germline chimeras were backcrossed for two generations to C57BL/6J.

Associated Images

Image 1
	Description: A Rosa26 RMCE plasmid constructed, made for use with the Rosa26 acceptor allele, was modified by replacing the sequences from -228 to +81 with a retinoic acid response element (DR5) fused to a TATA-CFP reporter. The resulting mouse ESCs will enable the feasibility of inserting signaling sentinel cassettes into a facilitating chromosomal locus to be directly tested. Reference: Not provided

Image 1

Description:

A Rosa26 RMCE plasmid constructed, made for use with the Rosa26 acceptor allele, was modified by replacing the sequences from -228 to +81 with a retinoic acid response element (DR5) fused to a TATA-CFP reporter. The resulting mouse ESCs will enable the feasibility of inserting signaling sentinel cassettes into a facilitating chromosomal locus to be directly tested.

Reference:
Not provided

Repositories

Magnuson Lab
Request this resource	Stock #: Not provided Availability Notes: Not provided

Contact Information

Preferred Contact
Name	Mark Magnuson
Institution	Vanderbilt University
Phone	615-322-7006
Email	mark.magnuson@vanderbilt.edu
Primary Lab Contact
Name	Not provided
Institution	Not provided
Phone	Not provided
Email	Not provided

Associated Publications

Publication	Citation
22888097	Serup P, Gustavsen C, Klein T, Potter LA, Lin R, Mullapudi N, Wandzioch E, Hines A, Davis A, Bruun C, Engberg N, Petersen DR, Peterslund JM, Macdonald RJ, Grapin-Botton A, Magnuson MA, Zaret KS Partial promoter substitutions generating transcriptional sentinels of diverse signaling pathways in embryonic stem cells and mice. (2012) Dis Model Mech 5: 956-66 (Added March 21, 2013)