TetO-CyclinD1-T286A/Cherry - Mouse Strain RES1221
Mouse Information
| Common Name: | TetO-CyclinD1-T286A/Cherry |
|---|---|
| MGI Official Name: | Gt(ROSA)26Sortm1(Ccnd1T286A)Stang |
| Description: | This strain expresses both mutant cyclin D1T286A and a cherry reporter under the regulation of a tetracycline-responsive bi-directional minimal CMV promoter (TRE-Tight; tetO). The cyclin D1-T286A mutant cannot be phosphorylated by GSK-3beta and is resistant to polyubiquitination. Therefore, cyclin D1-T286A remains nuclear throughout the cell cycle and has an extended half-life relative to wild-type cyclin D1. This strain represents an effective tool for generating inducible tissue-specific cyclin D1T286A mutants with a cherry reporter. |
| Categories: |
Tet |
Genetic Alterations
| 1) RMCE Targeted Mutagenesis | |
|---|---|
| Type of Allele | Cassette Acceptor |
| Targeted Gene | gene trap ROSA 26, Philippe Soriano (Gt(ROSA)26Sor - NCBI GeneID:14910) |
| Targeted Allele |
targeted mutation 1
(Rosa26tm1(LCA) - MGI:104735)
|
| Description of Targeting Vector | Not available |
| Targeting Vector Genbank File |
pRosa26.LCA.gb |
| Recombinase-Mediated Cassette Exchange Stage | |
| Type of Allele: | Conditional Activating |
| Exchanged Cassette Gene | Cyclin D1 (Ccnd1T286A - NCBI GeneID:12443) |
| Exchanged Cassette Allele Name | Rosa26(Ccnd1T286A) |
| Description of Exchange Vector | Flag-cyclinD1T286A was PCR amplified from plasmid (Diehl JA, et al., Genes Dev 1997;11:957 -972.). MluI restriction sites were added to 5' and 3' ends and a Kozak consensus sequence was added. PCR product was cloned into MluI sites of pBR322.hygro.ptight.cherry, screened for orientation, and sequence confirmed bi-directionally. |
| Exchange Vector Genbank File: | pBR322.hygro.ptight.cherry.CyclinD1T286A.gb |
| Citations | Not Available |
Strain Information
| Strain Type: | Mixed |
|---|---|
| Chimera/Founder Genetic Background: | 129S6/SvEvTac |
| Current Genetic Background: | C57BL/6J (date recorded: Not provided) |
| Strain Description: | Not provided |
Associated Images
| No associated images have been supplied | |
Repositories
No repositories indicated.Contact Information
| Preferred Contact | |
|---|---|
| Name | Ben Stanger |
| Institution | University of Pennsylvania |
| Phone | 215-746-5560 |
| bstanger@exchange.upenn.edu | |
| Primary Lab Contact | |
| Name | Matthew Emmett |
| Institution | University of Pennsylvania |
| Phone | Not provided |
| memmett@mail.med.upenn.edu | |
Associated Publications
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