H9 hESC directed differentiation following Novocell protocol - Study GBCO3365

Genomics Study Specifications

Study Name

H9 hESC directed differentiation following Novocell protocol

Contact Name

Mark Magnuson (Vanderbilt University)

Publication

Not provided

My Strategies

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Classification

Cell differentiation; Targets and roles of transcriptional regulators; Differentiation of insulin-producing cells

Links

Biomaterials Graph

BCBC Release Date

February 09, 2009

Public Release Date

July 29, 2011

Citation

unavailable

Differentiation Protocol Display

Synopsis

Study Description
Goals
Approaches
Results
Conclusions
Related Studies

Human embryonic stem cells (WiCell H9 passage 52) were differentiated following the Novocell protocol (D'Amour et al. Nature Biotechnology, 2005). The two biological replicates (Trial 15 and Trial 16) were analyzed by Real-Time PCR utilizing TaqMan Low Density Array Cards that have been customized for a set of 46 genes specific for endoderm and pancreatic gene expression.

No goal information provided

No approach information provided

No result information provided

No conclusion information provided

ES cell differentiation: 2800, 3365, 3425, 3668, 4216, 4314, 4574,

Platform types

Expression RT-PCR, Expression

Platforms

Show platform qPCR Primer Set (Magnuson)

Study Design Type

compound_treatment_design

Study Factors

Show study factors

Study factor ID	Name	Type
3524	Activin A start	compound_based_treatment
3509	Activin A end	compound_based_treatment
3537	DMEM start	compound_based_treatment
3536	FGF-7 end	compound_based_treatment
3535	HGF start	compound_based_treatment
3534	Retinoic Acid start	compound_based_treatment
3533	DAPT start	compound_based_treatment
3532	Hiclone FBS - early low end	compound_based_treatment
3531	Wnt3A end	compound_based_treatment
3530	DMEM end	compound_based_treatment
3529	CMRL start	compound_based_treatment
3528	RPMI end	compound_based_treatment
3527	DAPT end	compound_based_treatment
3526	Hiclone FBS - late high start	compound_based_treatment
3525	Exendin-4 start	compound_based_treatment
3508	KAAD-CYCLOPAMINE end	compound_based_treatment
3523	IGF1 end	compound_based_treatment
3522	CMRL end	compound_based_treatment
3521	FGF-7 start	compound_based_treatment
3520	Hiclone FBS - early low start	compound_based_treatment
3519	Wnt3A start	compound_based_treatment
3518	Exendin-4 end	compound_based_treatment
3517	HGF end	compound_based_treatment
3516	Retinoic Acid end	compound_based_treatment
3515	B27 end	compound_based_treatment
3514	RPMI start	compound_based_treatment
3513	KAAD-CYCLOPAMINE start	compound_based_treatment
3512	B27 start	compound_based_treatment
3511	IGF1 start	compound_based_treatment
3510	Hiclone FBS - late high end	compound_based_treatment

Study Assays

Show study assays

ID	Assay name	Study factor name	Study factor value
12927	D10 T15	KAAD-CYCLOPAMINE end	9
12927	D10 T15	Activin A end	3
12927	D10 T15	Hiclone FBS - late high end	6
12927	D10 T15	IGF1 start	9
12927	D10 T15	B27 start	6
12927	D10 T15	KAAD-CYCLOPAMINE start	3
12927	D10 T15	RPMI start	0
12927	D10 T15	B27 end	10
12927	D10 T15	Retinoic Acid end	9
12927	D10 T15	Exendin-4 end	10
12927	D10 T15	Wnt3A start	0
12927	D10 T15	Hiclone FBS - early low start	1
12927	D10 T15	FGF-7 start	3
12927	D10 T15	IGF1 end	10
12927	D10 T15	Activin A start	0
12927	D10 T15	Exendin-4 start	9
12927	D10 T15	Hiclone FBS - late high start	4
12927	D10 T15	DAPT end	10
12927	D10 T15	RPMI end	3
12927	D10 T15	DMEM end	10
12927	D10 T15	Wnt3A end	1
12927	D10 T15	Hiclone FBS - early low end	3
12927	D10 T15	DAPT start	9
12927	D10 T15	Retinoic Acid start	6
12927	D10 T15	FGF-7 end	9
12927	D10 T15	DMEM start	4
12928	D3 T16	KAAD-CYCLOPAMINE end	3
12928	D3 T16	Activin A end	3
12928	D3 T16	KAAD-CYCLOPAMINE start	3
12928	D3 T16	RPMI start	0
12928	D3 T16	Wnt3A start	0
12928	D3 T16	Hiclone FBS - early low start	1
12928	D3 T16	FGF-7 start	3
12928	D3 T16	Activin A start	0
12928	D3 T16	RPMI end	3
12928	D3 T16	Wnt3A end	1
12928	D3 T16	Hiclone FBS - early low end	3
12928	D3 T16	FGF-7 end	3
12929	D5 T16	KAAD-CYCLOPAMINE end	5
12929	D5 T16	Activin A end	3
12929	D5 T16	Hiclone FBS - late high end	5
12929	D5 T16	KAAD-CYCLOPAMINE start	3
12929	D5 T16	RPMI start	0
12929	D5 T16	Wnt3A start	0
12929	D5 T16	Hiclone FBS - early low start	1
12929	D5 T16	FGF-7 start	3
12929	D5 T16	Activin A start	0
12929	D5 T16	Hiclone FBS - late high start	4
12929	D5 T16	RPMI end	3
12929	D5 T16	DMEM end	5
12929	D5 T16	Wnt3A end	1
12929	D5 T16	Hiclone FBS - early low end	3
12929	D5 T16	FGF-7 end	5
12929	D5 T16	DMEM start	4
12930	D11 T15	KAAD-CYCLOPAMINE end	9
12930	D11 T15	Activin A end	3
12930	D11 T15	Hiclone FBS - late high end	6
12930	D11 T15	IGF1 start	9
12930	D11 T15	B27 start	6
12930	D11 T15	KAAD-CYCLOPAMINE start	3
12930	D11 T15	RPMI start	0
12930	D11 T15	B27 end	11
12930	D11 T15	Retinoic Acid end	9
12930	D11 T15	Exendin-4 end	11
12930	D11 T15	Wnt3A start	0
12930	D11 T15	Hiclone FBS - early low start	1
12930	D11 T15	FGF-7 start	3
12930	D11 T15	IGF1 end	11
12930	D11 T15	Activin A start	0
12930	D11 T15	Exendin-4 start	9
12930	D11 T15	Hiclone FBS - late high start	4
12930	D11 T15	DAPT end	11
12930	D11 T15	RPMI end	3
12930	D11 T15	DMEM end	11
12930	D11 T15	Wnt3A end	1
12930	D11 T15	Hiclone FBS - early low end	3
12930	D11 T15	DAPT start	9
12930	D11 T15	Retinoic Acid start	6
12930	D11 T15	FGF-7 end	9
12930	D11 T15	DMEM start	4
12931	D5 T15	KAAD-CYCLOPAMINE end	5
12931	D5 T15	Activin A end	3
12931	D5 T15	Hiclone FBS - late high end	5
12931	D5 T15	KAAD-CYCLOPAMINE start	3
12931	D5 T15	RPMI start	0
12931	D5 T15	Wnt3A start	0
12931	D5 T15	Hiclone FBS - early low start	1
12931	D5 T15	FGF-7 start	3
12931	D5 T15	Activin A start	0
12931	D5 T15	Hiclone FBS - late high start	4
12931	D5 T15	RPMI end	3
12931	D5 T15	DMEM end	5
12931	D5 T15	Wnt3A end	1
12931	D5 T15	Hiclone FBS - early low end	3
12931	D5 T15	FGF-7 end	5
12931	D5 T15	DMEM start	4
12932	D8 T16	KAAD-CYCLOPAMINE end	8
12932	D8 T16	Activin A end	3
12932	D8 T16	Hiclone FBS - late high end	6
12932	D8 T16	B27 start	6
12932	D8 T16	KAAD-CYCLOPAMINE start	3
12932	D8 T16	RPMI start	0
12932	D8 T16	B27 end	8
12932	D8 T16	Retinoic Acid end	8
12932	D8 T16	Wnt3A start	0
12932	D8 T16	Hiclone FBS - early low start	1
12932	D8 T16	FGF-7 start	3
12932	D8 T16	Activin A start	0
12932	D8 T16	Hiclone FBS - late high start	4
12932	D8 T16	RPMI end	3
12932	D8 T16	DMEM end	8
12932	D8 T16	Wnt3A end	1
12932	D8 T16	Hiclone FBS - early low end	3
12932	D8 T16	Retinoic Acid start	6
12932	D8 T16	FGF-7 end	8
12932	D8 T16	DMEM start	4
12933	D14 T15	KAAD-CYCLOPAMINE end	9
12933	D14 T15	Activin A end	3
12933	D14 T15	Hiclone FBS - late high end	6
12933	D14 T15	IGF1 start	9
12933	D14 T15	B27 start	6
12933	D14 T15	KAAD-CYCLOPAMINE start	3
12933	D14 T15	RPMI start	0
12933	D14 T15	B27 end	14
12933	D14 T15	Retinoic Acid end	9
12933	D14 T15	HGF end	14
12933	D14 T15	Exendin-4 end	14
12933	D14 T15	Wnt3A start	0
12933	D14 T15	Hiclone FBS - early low start	1
12933	D14 T15	FGF-7 start	3
12933	D14 T15	CMRL end	14
12933	D14 T15	IGF1 end	14
12933	D14 T15	Activin A start	0
12933	D14 T15	Exendin-4 start	9
12933	D14 T15	Hiclone FBS - late high start	4
12933	D14 T15	DAPT end	12
12933	D14 T15	RPMI end	3
12933	D14 T15	CMRL start	12
12933	D14 T15	DMEM end	12
12933	D14 T15	Wnt3A end	1
12933	D14 T15	Hiclone FBS - early low end	3
12933	D14 T15	DAPT start	9
12933	D14 T15	Retinoic Acid start	6
12933	D14 T15	HGF start	12
12933	D14 T15	FGF-7 end	9
12933	D14 T15	DMEM start	4
12934	D15 T16	KAAD-CYCLOPAMINE end	9
12934	D15 T16	Activin A end	3
12934	D15 T16	Hiclone FBS - late high end	6
12934	D15 T16	IGF1 start	9
12934	D15 T16	B27 start	6
12934	D15 T16	KAAD-CYCLOPAMINE start	3
12934	D15 T16	RPMI start	0
12934	D15 T16	B27 end	15
12934	D15 T16	Retinoic Acid end	9
12934	D15 T16	HGF end	15
12934	D15 T16	Exendin-4 end	15
12934	D15 T16	Wnt3A start	0
12934	D15 T16	Hiclone FBS - early low start	1
12934	D15 T16	FGF-7 start	3
12934	D15 T16	CMRL end	15
12934	D15 T16	IGF1 end	15
12934	D15 T16	Activin A start	0
12934	D15 T16	Exendin-4 start	9
12934	D15 T16	Hiclone FBS - late high start	4
12934	D15 T16	DAPT end	12
12934	D15 T16	RPMI end	3
12934	D15 T16	CMRL start	12
12934	D15 T16	DMEM end	12
12934	D15 T16	Wnt3A end	1
12934	D15 T16	Hiclone FBS - early low end	3
12934	D15 T16	DAPT start	9
12934	D15 T16	Retinoic Acid start	6
12934	D15 T16	HGF start	12
12934	D15 T16	FGF-7 end	9
12934	D15 T16	DMEM start	4
12935	D13 T16	KAAD-CYCLOPAMINE end	9
12935	D13 T16	Activin A end	3
12935	D13 T16	Hiclone FBS - late high end	6
12935	D13 T16	IGF1 start	9
12935	D13 T16	B27 start	6
12935	D13 T16	KAAD-CYCLOPAMINE start	3
12935	D13 T16	RPMI start	0
12935	D13 T16	B27 end	13
12935	D13 T16	Retinoic Acid end	9
12935	D13 T16	HGF end	13
12935	D13 T16	Exendin-4 end	13
12935	D13 T16	Wnt3A start	0
12935	D13 T16	Hiclone FBS - early low start	1
12935	D13 T16	FGF-7 start	3
12935	D13 T16	CMRL end	13
12935	D13 T16	IGF1 end	13
12935	D13 T16	Activin A start	0
12935	D13 T16	Exendin-4 start	9
12935	D13 T16	Hiclone FBS - late high start	4
12935	D13 T16	DAPT end	12
12935	D13 T16	RPMI end	3
12935	D13 T16	CMRL start	12
12935	D13 T16	DMEM end	12
12935	D13 T16	Wnt3A end	1
12935	D13 T16	Hiclone FBS - early low end	3
12935	D13 T16	DAPT start	9
12935	D13 T16	Retinoic Acid start	6
12935	D13 T16	HGF start	12
12935	D13 T16	FGF-7 end	9
12935	D13 T16	DMEM start	4
12936	D9 T16	KAAD-CYCLOPAMINE end	9
12936	D9 T16	Activin A end	3
12936	D9 T16	Hiclone FBS - late high end	6
12936	D9 T16	IGF1 start	9
12936	D9 T16	B27 start	6
12936	D9 T16	KAAD-CYCLOPAMINE start	3
12936	D9 T16	RPMI start	0
12936	D9 T16	B27 end	9
12936	D9 T16	Retinoic Acid end	9
12936	D9 T16	Exendin-4 end	9
12936	D9 T16	Wnt3A start	0
12936	D9 T16	Hiclone FBS - early low start	1
12936	D9 T16	FGF-7 start	3
12936	D9 T16	IGF1 end	9
12936	D9 T16	Activin A start	0
12936	D9 T16	Exendin-4 start	9
12936	D9 T16	Hiclone FBS - late high start	4
12936	D9 T16	DAPT end	9
12936	D9 T16	RPMI end	3
12936	D9 T16	DMEM end	9
12936	D9 T16	Wnt3A end	1
12936	D9 T16	Hiclone FBS - early low end	3
12936	D9 T16	DAPT start	9
12936	D9 T16	Retinoic Acid start	6
12936	D9 T16	FGF-7 end	9
12936	D9 T16	DMEM start	4
12937	D3 T15	KAAD-CYCLOPAMINE end	3
12937	D3 T15	Activin A end	3
12937	D3 T15	KAAD-CYCLOPAMINE start	3
12937	D3 T15	RPMI start	0
12937	D3 T15	Wnt3A start	0
12937	D3 T15	Hiclone FBS - early low start	1
12937	D3 T15	FGF-7 start	3
12937	D3 T15	Activin A start	0
12937	D3 T15	RPMI end	3
12937	D3 T15	Wnt3A end	1
12937	D3 T15	Hiclone FBS - early low end	3
12937	D3 T15	FGF-7 end	3
12938	D12 T16	KAAD-CYCLOPAMINE end	9
12938	D12 T16	Activin A end	3
12938	D12 T16	Hiclone FBS - late high end	6
12938	D12 T16	IGF1 start	9
12938	D12 T16	B27 start	6
12938	D12 T16	KAAD-CYCLOPAMINE start	3
12938	D12 T16	RPMI start	0
12938	D12 T16	B27 end	12
12938	D12 T16	Retinoic Acid end	9
12938	D12 T16	HGF end	12
12938	D12 T16	Exendin-4 end	12
12938	D12 T16	Wnt3A start	0
12938	D12 T16	Hiclone FBS - early low start	1
12938	D12 T16	FGF-7 start	3
12938	D12 T16	CMRL end	12
12938	D12 T16	IGF1 end	12
12938	D12 T16	Activin A start	0
12938	D12 T16	Exendin-4 start	9
12938	D12 T16	Hiclone FBS - late high start	4
12938	D12 T16	DAPT end	12
12938	D12 T16	RPMI end	3
12938	D12 T16	CMRL start	12
12938	D12 T16	DMEM end	12
12938	D12 T16	Wnt3A end	1
12938	D12 T16	Hiclone FBS - early low end	3
12938	D12 T16	DAPT start	9
12938	D12 T16	Retinoic Acid start	6
12938	D12 T16	HGF start	12
12938	D12 T16	FGF-7 end	9
12938	D12 T16	DMEM start	4
12939	D6 T16	KAAD-CYCLOPAMINE end	6
12939	D6 T16	Activin A end	3
12939	D6 T16	Hiclone FBS - late high end	6
12939	D6 T16	B27 start	6
12939	D6 T16	KAAD-CYCLOPAMINE start	3
12939	D6 T16	RPMI start	0
12939	D6 T16	B27 end	6
12939	D6 T16	Retinoic Acid end	6
12939	D6 T16	Wnt3A start	0
12939	D6 T16	Hiclone FBS - early low start	1
12939	D6 T16	FGF-7 start	3
12939	D6 T16	Activin A start	0
12939	D6 T16	Hiclone FBS - late high start	4
12939	D6 T16	RPMI end	3
12939	D6 T16	DMEM end	6
12939	D6 T16	Wnt3A end	1
12939	D6 T16	Hiclone FBS - early low end	3
12939	D6 T16	Retinoic Acid start	6
12939	D6 T16	FGF-7 end	6
12939	D6 T16	DMEM start	4
12940	D10 T16	KAAD-CYCLOPAMINE end	9
12940	D10 T16	Activin A end	3
12940	D10 T16	Hiclone FBS - late high end	6
12940	D10 T16	IGF1 start	9
12940	D10 T16	B27 start	6
12940	D10 T16	KAAD-CYCLOPAMINE start	3
12940	D10 T16	RPMI start	0
12940	D10 T16	B27 end	10
12940	D10 T16	Retinoic Acid end	9
12940	D10 T16	Exendin-4 end	10
12940	D10 T16	Wnt3A start	0
12940	D10 T16	Hiclone FBS - early low start	1
12940	D10 T16	FGF-7 start	3
12940	D10 T16	IGF1 end	10
12940	D10 T16	Activin A start	0
12940	D10 T16	Exendin-4 start	9
12940	D10 T16	Hiclone FBS - late high start	4
12940	D10 T16	DAPT end	10
12940	D10 T16	RPMI end	3
12940	D10 T16	DMEM end	10
12940	D10 T16	Wnt3A end	1
12940	D10 T16	Hiclone FBS - early low end	3
12940	D10 T16	DAPT start	9
12940	D10 T16	Retinoic Acid start	6
12940	D10 T16	FGF-7 end	9
12940	D10 T16	DMEM start	4
12941	D0 T16	Activin A end	0
12941	D0 T16	RPMI start	0
12941	D0 T16	Wnt3A start	0
12941	D0 T16	Activin A start	0
12941	D0 T16	RPMI end	0
12941	D0 T16	Wnt3A end	0
12942	D7 T15	KAAD-CYCLOPAMINE end	7
12942	D7 T15	Activin A end	3
12942	D7 T15	Hiclone FBS - late high end	6
12942	D7 T15	B27 start	6
12942	D7 T15	KAAD-CYCLOPAMINE start	3
12942	D7 T15	RPMI start	0
12942	D7 T15	B27 end	7
12942	D7 T15	Retinoic Acid end	7
12942	D7 T15	Wnt3A start	0
12942	D7 T15	Hiclone FBS - early low start	1
12942	D7 T15	FGF-7 start	3
12942	D7 T15	Activin A start	0
12942	D7 T15	Hiclone FBS - late high start	4
12942	D7 T15	RPMI end	3
12942	D7 T15	DMEM end	7
12942	D7 T15	Wnt3A end	1
12942	D7 T15	Hiclone FBS - early low end	3
12942	D7 T15	Retinoic Acid start	6
12942	D7 T15	FGF-7 end	7
12942	D7 T15	DMEM start	4
12943	D7 T16	KAAD-CYCLOPAMINE end	7
12943	D7 T16	Activin A end	3
12943	D7 T16	Hiclone FBS - late high end	6
12943	D7 T16	B27 start	6
12943	D7 T16	KAAD-CYCLOPAMINE start	3
12943	D7 T16	RPMI start	0
12943	D7 T16	B27 end	7
12943	D7 T16	Retinoic Acid end	7
12943	D7 T16	Wnt3A start	0
12943	D7 T16	Hiclone FBS - early low start	1
12943	D7 T16	FGF-7 start	3
12943	D7 T16	Activin A start	0
12943	D7 T16	Hiclone FBS - late high start	4
12943	D7 T16	RPMI end	3
12943	D7 T16	DMEM end	7
12943	D7 T16	Wnt3A end	1
12943	D7 T16	Hiclone FBS - early low end	3
12943	D7 T16	Retinoic Acid start	6
12943	D7 T16	FGF-7 end	7
12943	D7 T16	DMEM start	4
12944	D15 T15	KAAD-CYCLOPAMINE end	9
12944	D15 T15	Activin A end	3
12944	D15 T15	Hiclone FBS - late high end	6
12944	D15 T15	IGF1 start	9
12944	D15 T15	B27 start	6
12944	D15 T15	KAAD-CYCLOPAMINE start	3
12944	D15 T15	RPMI start	0
12944	D15 T15	B27 end	15
12944	D15 T15	Retinoic Acid end	9
12944	D15 T15	HGF end	15
12944	D15 T15	Exendin-4 end	15
12944	D15 T15	Wnt3A start	0
12944	D15 T15	Hiclone FBS - early low start	1
12944	D15 T15	FGF-7 start	3
12944	D15 T15	CMRL end	15
12944	D15 T15	IGF1 end	15
12944	D15 T15	Activin A start	0
12944	D15 T15	Exendin-4 start	9
12944	D15 T15	Hiclone FBS - late high start	4
12944	D15 T15	DAPT end	12
12944	D15 T15	RPMI end	3
12944	D15 T15	CMRL start	12
12944	D15 T15	DMEM end	12
12944	D15 T15	Wnt3A end	1
12944	D15 T15	Hiclone FBS - early low end	3
12944	D15 T15	DAPT start	9
12944	D15 T15	Retinoic Acid start	6
12944	D15 T15	HGF start	12
12944	D15 T15	FGF-7 end	9
12944	D15 T15	DMEM start	4
12945	D1 T16	Activin A end	1
12945	D1 T16	RPMI start	0
12945	D1 T16	Wnt3A start	0
12945	D1 T16	Hiclone FBS - early low start	1
12945	D1 T16	Activin A start	0
12945	D1 T16	RPMI end	1
12945	D1 T16	Wnt3A end	1
12945	D1 T16	Hiclone FBS - early low end	1
12946	D13 T15	KAAD-CYCLOPAMINE end	9
12946	D13 T15	Activin A end	3
12946	D13 T15	Hiclone FBS - late high end	6
12946	D13 T15	IGF1 start	9
12946	D13 T15	B27 start	6
12946	D13 T15	KAAD-CYCLOPAMINE start	3
12946	D13 T15	RPMI start	0
12946	D13 T15	B27 end	13
12946	D13 T15	Retinoic Acid end	9
12946	D13 T15	HGF end	13
12946	D13 T15	Exendin-4 end	13
12946	D13 T15	Wnt3A start	0
12946	D13 T15	Hiclone FBS - early low start	1
12946	D13 T15	FGF-7 start	3
12946	D13 T15	CMRL end	13
12946	D13 T15	IGF1 end	13
12946	D13 T15	Activin A start	0
12946	D13 T15	Exendin-4 start	9
12946	D13 T15	Hiclone FBS - late high start	4
12946	D13 T15	DAPT end	12
12946	D13 T15	RPMI end	3
12946	D13 T15	CMRL start	12
12946	D13 T15	DMEM end	12
12946	D13 T15	Wnt3A end	1
12946	D13 T15	Hiclone FBS - early low end	3
12946	D13 T15	DAPT start	9
12946	D13 T15	Retinoic Acid start	6
12946	D13 T15	HGF start	12
12946	D13 T15	FGF-7 end	9
12946	D13 T15	DMEM start	4
12947	D6 T15	KAAD-CYCLOPAMINE end	6
12947	D6 T15	Activin A end	3
12947	D6 T15	Hiclone FBS - late high end	6
12947	D6 T15	B27 start	6
12947	D6 T15	KAAD-CYCLOPAMINE start	3
12947	D6 T15	RPMI start	0
12947	D6 T15	B27 end	6
12947	D6 T15	Retinoic Acid end	6
12947	D6 T15	Wnt3A start	0
12947	D6 T15	Hiclone FBS - early low start	1
12947	D6 T15	FGF-7 start	3
12947	D6 T15	Activin A start	0
12947	D6 T15	Hiclone FBS - late high start	4
12947	D6 T15	RPMI end	3
12947	D6 T15	DMEM end	6
12947	D6 T15	Wnt3A end	1
12947	D6 T15	Hiclone FBS - early low end	3
12947	D6 T15	Retinoic Acid start	6
12947	D6 T15	FGF-7 end	6
12947	D6 T15	DMEM start	4
12948	D2 T16	Activin A end	2
12948	D2 T16	RPMI start	0
12948	D2 T16	Wnt3A start	0
12948	D2 T16	Hiclone FBS - early low start	1
12948	D2 T16	Activin A start	0
12948	D2 T16	RPMI end	2
12948	D2 T16	Wnt3A end	1
12948	D2 T16	Hiclone FBS - early low end	2
12949	D2 T15	Activin A end	2
12949	D2 T15	RPMI start	0
12949	D2 T15	Wnt3A start	0
12949	D2 T15	Hiclone FBS - early low start	1
12949	D2 T15	Activin A start	0
12949	D2 T15	RPMI end	2
12949	D2 T15	Wnt3A end	1
12949	D2 T15	Hiclone FBS - early low end	2
12950	D0 T15	Activin A end	0
12950	D0 T15	RPMI start	0
12950	D0 T15	Wnt3A start	0
12950	D0 T15	Activin A start	0
12950	D0 T15	RPMI end	0
12950	D0 T15	Wnt3A end	0
12951	D12 T15	KAAD-CYCLOPAMINE end	9
12951	D12 T15	Activin A end	3
12951	D12 T15	Hiclone FBS - late high end	6
12951	D12 T15	IGF1 start	9
12951	D12 T15	B27 start	6
12951	D12 T15	KAAD-CYCLOPAMINE start	3
12951	D12 T15	RPMI start	0
12951	D12 T15	B27 end	12
12951	D12 T15	Retinoic Acid end	9
12951	D12 T15	HGF end	12
12951	D12 T15	Exendin-4 end	12
12951	D12 T15	Wnt3A start	0
12951	D12 T15	Hiclone FBS - early low start	1
12951	D12 T15	FGF-7 start	3
12951	D12 T15	CMRL end	12
12951	D12 T15	IGF1 end	12
12951	D12 T15	Activin A start	0
12951	D12 T15	Exendin-4 start	9
12951	D12 T15	Hiclone FBS - late high start	4
12951	D12 T15	DAPT end	12
12951	D12 T15	RPMI end	3
12951	D12 T15	CMRL start	12
12951	D12 T15	DMEM end	12
12951	D12 T15	Wnt3A end	1
12951	D12 T15	Hiclone FBS - early low end	3
12951	D12 T15	DAPT start	9
12951	D12 T15	Retinoic Acid start	6
12951	D12 T15	HGF start	12
12951	D12 T15	FGF-7 end	9
12951	D12 T15	DMEM start	4
12952	D9 T15	KAAD-CYCLOPAMINE end	9
12952	D9 T15	Activin A end	3
12952	D9 T15	Hiclone FBS - late high end	6
12952	D9 T15	IGF1 start	9
12952	D9 T15	B27 start	6
12952	D9 T15	KAAD-CYCLOPAMINE start	3
12952	D9 T15	RPMI start	0
12952	D9 T15	B27 end	9
12952	D9 T15	Retinoic Acid end	9
12952	D9 T15	Exendin-4 end	9
12952	D9 T15	Wnt3A start	0
12952	D9 T15	Hiclone FBS - early low start	1
12952	D9 T15	FGF-7 start	3
12952	D9 T15	IGF1 end	9
12952	D9 T15	Activin A start	0
12952	D9 T15	Exendin-4 start	9
12952	D9 T15	Hiclone FBS - late high start	4
12952	D9 T15	DAPT end	9
12952	D9 T15	RPMI end	3
12952	D9 T15	DMEM end	9
12952	D9 T15	Wnt3A end	1
12952	D9 T15	Hiclone FBS - early low end	3
12952	D9 T15	DAPT start	9
12952	D9 T15	Retinoic Acid start	6
12952	D9 T15	FGF-7 end	9
12952	D9 T15	DMEM start	4
12953	D1 T15	Activin A end	1
12953	D1 T15	RPMI start	0
12953	D1 T15	Wnt3A start	0
12953	D1 T15	Hiclone FBS - early low start	1
12953	D1 T15	Activin A start	0
12953	D1 T15	RPMI end	1
12953	D1 T15	Wnt3A end	1
12953	D1 T15	Hiclone FBS - early low end	1
12954	D14 T16	KAAD-CYCLOPAMINE end	9
12954	D14 T16	Activin A end	3
12954	D14 T16	Hiclone FBS - late high end	6
12954	D14 T16	IGF1 start	9
12954	D14 T16	B27 start	6
12954	D14 T16	KAAD-CYCLOPAMINE start	3
12954	D14 T16	RPMI start	0
12954	D14 T16	B27 end	14
12954	D14 T16	Retinoic Acid end	9
12954	D14 T16	HGF end	14
12954	D14 T16	Exendin-4 end	14
12954	D14 T16	Wnt3A start	0
12954	D14 T16	Hiclone FBS - early low start	1
12954	D14 T16	FGF-7 start	3
12954	D14 T16	CMRL end	14
12954	D14 T16	IGF1 end	14
12954	D14 T16	Activin A start	0
12954	D14 T16	Exendin-4 start	9
12954	D14 T16	Hiclone FBS - late high start	4
12954	D14 T16	DAPT end	12
12954	D14 T16	RPMI end	3
12954	D14 T16	CMRL start	12
12954	D14 T16	DMEM end	12
12954	D14 T16	Wnt3A end	1
12954	D14 T16	Hiclone FBS - early low end	3
12954	D14 T16	DAPT start	9
12954	D14 T16	Retinoic Acid start	6
12954	D14 T16	HGF start	12
12954	D14 T16	FGF-7 end	9
12954	D14 T16	DMEM start	4
12955	D11 T16	KAAD-CYCLOPAMINE end	9
12955	D11 T16	Activin A end	3
12955	D11 T16	Hiclone FBS - late high end	6
12955	D11 T16	IGF1 start	9
12955	D11 T16	B27 start	6
12955	D11 T16	KAAD-CYCLOPAMINE start	3
12955	D11 T16	RPMI start	0
12955	D11 T16	B27 end	11
12955	D11 T16	Retinoic Acid end	9
12955	D11 T16	Exendin-4 end	11
12955	D11 T16	Wnt3A start	0
12955	D11 T16	Hiclone FBS - early low start	1
12955	D11 T16	FGF-7 start	3
12955	D11 T16	IGF1 end	11
12955	D11 T16	Activin A start	0
12955	D11 T16	Exendin-4 start	9
12955	D11 T16	Hiclone FBS - late high start	4
12955	D11 T16	DAPT end	11
12955	D11 T16	RPMI end	3
12955	D11 T16	DMEM end	11
12955	D11 T16	Wnt3A end	1
12955	D11 T16	Hiclone FBS - early low end	3
12955	D11 T16	DAPT start	9
12955	D11 T16	Retinoic Acid start	6
12955	D11 T16	FGF-7 end	9
12955	D11 T16	DMEM start	4
12956	D4 T16	KAAD-CYCLOPAMINE end	4
12956	D4 T16	Activin A end	3
12956	D4 T16	Hiclone FBS - late high end	4
12956	D4 T16	KAAD-CYCLOPAMINE start	3
12956	D4 T16	RPMI start	0
12956	D4 T16	Wnt3A start	0
12956	D4 T16	Hiclone FBS - early low start	1
12956	D4 T16	FGF-7 start	3
12956	D4 T16	Activin A start	0
12956	D4 T16	Hiclone FBS - late high start	4
12956	D4 T16	RPMI end	3
12956	D4 T16	DMEM end	4
12956	D4 T16	Wnt3A end	1
12956	D4 T16	Hiclone FBS - early low end	3
12956	D4 T16	FGF-7 end	4
12956	D4 T16	DMEM start	4
12957	D4 T15	KAAD-CYCLOPAMINE end	4
12957	D4 T15	Activin A end	3
12957	D4 T15	Hiclone FBS - late high end	4
12957	D4 T15	KAAD-CYCLOPAMINE start	3
12957	D4 T15	RPMI start	0
12957	D4 T15	Wnt3A start	0
12957	D4 T15	Hiclone FBS - early low start	1
12957	D4 T15	FGF-7 start	3
12957	D4 T15	Activin A start	0
12957	D4 T15	Hiclone FBS - late high start	4
12957	D4 T15	RPMI end	3
12957	D4 T15	DMEM end	4
12957	D4 T15	Wnt3A end	1
12957	D4 T15	Hiclone FBS - early low end	3
12957	D4 T15	FGF-7 end	4
12957	D4 T15	DMEM start	4
12958	D8 T15	KAAD-CYCLOPAMINE end	8
12958	D8 T15	Activin A end	3
12958	D8 T15	Hiclone FBS - late high end	6
12958	D8 T15	B27 start	6
12958	D8 T15	KAAD-CYCLOPAMINE start	3
12958	D8 T15	RPMI start	0
12958	D8 T15	B27 end	8
12958	D8 T15	Retinoic Acid end	8
12958	D8 T15	Wnt3A start	0
12958	D8 T15	Hiclone FBS - early low start	1
12958	D8 T15	FGF-7 start	3
12958	D8 T15	Activin A start	0
12958	D8 T15	Hiclone FBS - late high start	4
12958	D8 T15	RPMI end	3
12958	D8 T15	DMEM end	8
12958	D8 T15	Wnt3A end	1
12958	D8 T15	Hiclone FBS - early low end	3
12958	D8 T15	Retinoic Acid start	6
12958	D8 T15	FGF-7 end	8
12958	D8 T15	DMEM start	4