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Transcriptional profiling of stress-response in cultured porcine islets - Study GBCO3244


Genomics Study Specifications

Study Name Transcriptional profiling of stress-response in cultured porcine islets
Contact Name Scott C. Fahrenkrug (Department of Animal Science - University Of Minnesota)
Publication http://www.ncbi.nlm.nih.gov/pubmed/17407763
My Strategies Return to My Strategies page
Classification Islet/beta-cell stimulation/injury; Cell stimulation/injury
Links
BCBC Release Date May 12, 2008
Public Release Date May 12, 2008
Citation Dvorak CM, Hårdstedt M, Xie H, Wang M, Papas KK, Hering BJ, Murtaugh MP, Fahrenkrug SC. Transcriptional profiling of stress response in cultured porcine islets. Biochem Biophys Res Commun. 2007. 357:118-25
Synopsis
An increasing demand for cell-based diabetes therapy could be met through xenotransplantation of adult porcine islets. Use of islet xenotranplantation on a large scale would require rigorous safety and quality control measures to maximize transplant success. Development of molecular tools to monitor porcine islet cellular responses to ischemic, osmotic, mechanical and oxidative stresses during islet cell processing and post-isolation culturing would aid the rational design of cytoprotective strategies aimed at improving transplant outcomes. In addition, gene expression signatures informative for islet quality could serve as an adjunct to physiological testing to establish the suitability of islet products for transplantation. Experiment Overall Design: Nine adult Landrace sows were sacrificed, the pancreases were dissected, and islet cells isolated as previously described. Islet preparation purity was assessed by light microscopy after staining with diphenylthiocarbazone and ranged between 90-95% for the preparations used. Profiles of islet cells cultured under standard conditions were compared to islet cells cultured under stress conditions with elevated glucose (16.7 mM) or addition of inflammatory cytokines (IL-1beta, TNF-alpha, and IFN-gamma), or both, for 48 hours.
Identify molecular markers of physiological and immunological stress in porcine islets in anticipation of their use in xenotransplantation.
Porcine islets were cultured under stress conditions of elevated glucose, inflammatory cytokine addition (IL-1beta, TNF-alpha, IFN-gamma), or both for 48 hours and gene expression changes monitored by microarray spotted with the Pig_Array_Ready Oligo set and quantitative RT-PCR.
Changes observed from hyperglycemia included increased thioredoxin interacting protein (TXNIP) and metabolic process mRNAs. Cytokine treatment increased expression of JAK-STAT pathway components, oxidative stress (transglutaminase 2 - TGM2), and beta-cell dysfunction genes. The transcription factor, C/EBPdelta was elevated by cytokine treatment. Apolipoprotein C3 and transthyretin have been identified as markers for type 2 diabetes and were also elevated as was MARCKS, a substrate of PKC and known to change in cells under diabetic conditions.
Thioredoxin interacting protein (TXNIP) and transglutamase 2 (TGM2, not previously identified in rodent or human studies) may be useful molecular markers of islet cell stress (hyperglycemia and cytokine stress, respectively).
Platform types Expression, Expression microarray
Platforms Show platform Pig_Array_Ready Oligo set
Study Design Type
  • compound_treatment_design
Study Factors Show study factors
Study Assays Show study assays


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Repositories

Stoeckert Lab
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Resource Tags

APOC3, apolipoprotein C-III TTR, CCAAT/enhancer binding protein (C/EBP), Cebpd, C/EBPd, delta, Pig_Array_Ready Oligo set v1.0, protein-glutamine-gamma-glutamyltransferase), TGM2, thioredoxin interacting protein, transglutaminase 2 (C polypeptide, transthyretin MARCKS myristoylated alanine-rich protein kinase C substrate, TXNIP

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Resource History & Actions

Approved on May 12, 2008
Last modified on Jan 17, 2012
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