Cyclophosphamide-induced beta Cell Destruction in NOD Mice - Study GBCO2000

Genomics Study Specifications

Study Name

Cyclophosphamide-induced beta Cell Destruction in NOD Mice

Contact Name

Christophe Benoist (Joslin Diabetes Center and Harvard Medical School)

Publication

http://www.ncbi.nlm.nih.gov/pubmed/15331540

My Strategies

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Classification

Islet/beta-cell stimulation/injury; Cell stimulation/injury

Links

Biomaterials Graph

BCBC Release Date

October 19, 2005

Public Release Date

October 19, 2005

Citation

Matos M, Park R, Mathis D, Benoist C. Progression to islet destruction in a cyclophosphamide-induced transgenic model: a microarray overview. Diabetes. 2004. 53:2310-21

Synopsis

Study Description
Goals
Approaches
Results
Conclusions
Related Studies

Type 1 diabetes appears to progress in a highly regulated manner and insulitis can persist for long periods of time before the terminal destruction of beta cells. To study the final stage of diabetogenesis, BDC2.5/NOD mice were treated with cyclophosphamide to induce type 1 diabetes. Pancreatic islets were analyzed using the Affymetrix MU74v2A microarray platform before treatment (Eight Samples at Day 0) and as treatment progressed (Four Samples at Day 1, Three Samples at Day 2, and Three Samples at Day 3).

No goal information provided

No approach information provided

No result information provided

No conclusion information provided

NOD mice: 2000, 2421, 2424, 2440, 3144,
stress and apoptosis in islets/ beta cells: 751, 2000, 2001, 2020, 2021, 2301, 2904, 3244, 3650, 3975, 3977, 3979, 3980,

Platform types

Expression microarray, Expression

Platforms

Show platform Affymetrix MG_U74A

Study Design Type

compound_treatment_design
time_series_design

Study Factors

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Study Assays

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ID	Assay name	Study factor name	Study factor value
6575	Day0.1 -GSM41439	Cyclophosphamide injection	0
6576	Day0.2 -GSM41440	Cyclophosphamide injection	0
6577	Day0.3 -GSM41441	Cyclophosphamide injection	0
6578	Day0.4 -GSM41442	Cyclophosphamide injection	0
6579	Day0.5 -GSM41443	Cyclophosphamide injection	0
6580	Day0.6 -GSM41444	Cyclophosphamide injection	0
6581	Day0.7 -GSM41445	Cyclophosphamide injection	0
6582	Day0.8 -GSM41446	Cyclophosphamide injection	0
6583	Day1.1 -GSM41447	Cyclophosphamide injection	0.02
6583	Day1.1 -GSM41447	collection time	24
6584	Day1.2 -GSM41448	Cyclophosphamide injection	0.02
6584	Day1.2 -GSM41448	collection time	24
6585	Day1.3 -GSM41449	Cyclophosphamide injection	0.02
6585	Day1.3 -GSM41449	collection time	24
6586	Day1.4 -GSM41450	Cyclophosphamide injection	0.02
6586	Day1.4 -GSM41450	collection time	24
6587	Day2.1 -GSM41451	Cyclophosphamide injection	0.02
6587	Day2.1 -GSM41451	collection time	48
6588	Day2.2 -GSM41452	Cyclophosphamide injection	0.02
6588	Day2.2 -GSM41452	collection time	48
6589	Day2.3 -GSM41453	Cyclophosphamide injection	0.02
6589	Day2.3 -GSM41453	collection time	48
6590	Day3.1 -GSM41454	Cyclophosphamide injection	0.02
6590	Day3.1 -GSM41454	collection time	72
6591	Day3.2 -GSM41455	Cyclophosphamide injection	0.02
6591	Day3.2 -GSM41455	collection time	72
6592	Day3.3 -GSM41456	Cyclophosphamide injection	0.02
6592	Day3.3 -GSM41456	collection time	72