Overexpression of Neurogenin3 in Mpac mouse ductal cells - Study GBCO1470

Genomics Study Specifications

Study Name

Overexpression of Neurogenin3 in Mpac mouse ductal cells

Contact Name

Caroline Mrejen

Publication

http://www.ncbi.nlm.nih.gov/pubmed/15340143

My Strategies

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Classification

Targets and roles of transcriptional regulators

Links

Biomaterials Graph

ArrayExpress

BCBC Release Date

November 03, 2004

Public Release Date

November 03, 2004

Citation

Gasa R, Mrejen C, Leachman N, Otten M, Barnes M, Wang J, Chakrabarti S, Mirmira R, German M. Proendocrine genes coordinate the pancreatic islet differentiation program in vitro. Proc Natl Acad Sci U S A. 2004. 101:13245-50

Synopsis

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We have developed an in vitro model of Ngn3-dependent differentiation by infecting pancreatic duct cell lines with an Ngn3-expressing adenovirus. We used glass microarrays containing 18,000 mouse cDNAs and compared gene expression patterns in mPAC L20 cells infected with either AdCMV-gal (control) or AdCMV-ngn3. All comparisons were performed 48 h after viral infection, meaning that genes differentially expressed in Ngn3-expressing mPAC cells could include genes whose expression was directly or indirectly controlled by Ngn3.

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