Panc-1 Cell Differentiation - Study GBCO670

Genomics Study Specifications

Study Name

Panc-1 Cell Differentiation

Contact Name

Marvin Gershengorn (NIDDK Intramural)

Publication

Not provided

My Strategies

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Classification

Cell differentiation; Differentiation of insulin-producing cells

Links

Biomaterials Graph

BCBC Release Date

December 02, 2003

Public Release Date

December 02, 2003

Citation

unavailable

Synopsis

Study Description
Goals
Approaches
Results
Conclusions
Related Studies

This study (Experiment) consists of 16 arrays.The purpose of our microarray experiment was to assess changes in gene expression during this differentiation program. The basic experiment was to compare gene expression in control cells cultured in SCM and in cells that had been cultured in SFM for 24 hours. Three biological replicates were prepared for each condition and one of the biological replicates was analyzed by microarray in triplicate, for a total of 5 array hybridizations for each time point. In the specific case of Affymetrix arrays, duplicate biological samples were also prepared from cells that had been exposed to SFM for 8 hours, 3 days, and 8 days.

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No result information provided

No conclusion information provided

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Platform types

Expression microarray, Expression

Platforms

Show platform Affymetrix HG_U95A

Study Design Type

development_or_differentiation_design
growth_condition_design

Study Factors

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Study Assays

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ID	Assay name	Study factor name	Study factor value
1854	C11 1008485 (Lot Number)	Is SFM treated	NO
1855	C12 1008485 (Lot Number)	Is SFM treated	NO
1856	C13 1008485 (Lot Number)	Is SFM treated	NO
1857	C2 1008485 (Lot Number)	Is SFM treated	NO
1858	C3 1008485 (Lot Number)	Is SFM treated	NO
1859	1D11 1008485 (Lot Number)	Is SFM treated	YES
1859	1D11 1008485 (Lot Number)	SFM exposure time	1
1860	1D12 1008485 (Lot Number)	Is SFM treated	YES
1860	1D12 1008485 (Lot Number)	SFM exposure time	1
1861	1D13 1008485 (Lot Number)	Is SFM treated	YES
1861	1D13 1008485 (Lot Number)	SFM exposure time	1
1862	1D2 1008485 (Lot Number)	Is SFM treated	YES
1862	1D2 1008485 (Lot Number)	SFM exposure time	1
1863	1D3 1008485 (Lot Number)	Is SFM treated	YES
1863	1D3 1008485 (Lot Number)	SFM exposure time	1
1864	8H1 1008485 (Lot Number)	Is SFM treated	YES
1864	8H1 1008485 (Lot Number)	SFM exposure time	8
1865	8H2 1008485 (Lot Number)	Is SFM treated	YES
1865	8H2 1008485 (Lot Number)	SFM exposure time	8
1866	3D1 1008485 (Lot Number)	Is SFM treated	YES
1866	3D1 1008485 (Lot Number)	SFM exposure time	3
1867	3D2 1008485 (Lot Number)	Is SFM treated	YES
1867	3D2 1008485 (Lot Number)	SFM exposure time	3
1868	8D1 1008485 (Lot Number)	Is SFM treated	YES
1868	8D1 1008485 (Lot Number)	SFM exposure time	8
1869	8D2 1008485 (Lot Number)	Is SFM treated	YES
1869	8D2 1008485 (Lot Number)	SFM exposure time	8