biotinTag-Ptf1a - Mouse Strain RES1001
Mouse Information
| Common Name: | biotinTag-Ptf1a |
|---|---|
| MGI Official Name: | Ptf1atm3Macd |
| Description: | Modified allele of Ptf1a with a biotinylation Tag sequence attached to the amino terminus of Ptf1a. |
| Categories: | None specified. |
Genetic Alterations
| 1) RMCE Targeted Mutagenesis | |
|---|---|
| Type of Allele | Gene Replacement |
| Targeted Gene | Pancreas transcription factor 1a (Ptf1a - NCBI GeneID:19213) |
| Targeted Allele |
targeted mutation 3
(Ptf1atm3Macd - MGI:1328312)
|
| Description of Targeting Vector | The vector contained a loxP site and an inverted loxP site flanking the targeting region that contained (in order) the following: Ptf1a gene sequence from -1.7Kb to +2.4Kb; an FRT site with a biotinylation tag sequence added to the N-terminus; the pgk-hydromycin resistance gene; an FRT site; the pgk-hygromycin resistance gene; an FRT site. The targeting vector was constructed from the base vector BS.LP.ILP/FRT2.Hygro. |
| Targeting Vector Genbank File |
Ptf1a.LCA.gb |
| Recombinase-Mediated Cassette Exchange Stage | |
| Type of Allele: | Not available |
| Exchanged Cassette Gene | Not provided. (MGI:19213) |
| Exchanged Cassette Allele Name | Ptf1aLCA |
| Description of Exchange Vector | Not provided |
| Exchange Vector Genbank File: | Biotin-Ptf1a-RMCE.gbk |
| Citations | Not Available |
Strain Information
| Strain Type: | Inbred Strain |
|---|---|
| Chimera/Founder Genetic Background: | 129 |
| Current Genetic Background: | Not provided (date recorded: Not provided) |
| Strain Description: | Not provided |
Associated Images
| Image 1 | |
|---|---|
|
Description: Schematic of the BiotinTag-Ptf1a cassette for exchange into the Ptf1a-LCA acceptor. The position of the Biotinylatable Tag sequence inserted at the N-terminus of the amino acid coding sequence of Ptf1a is indicated. The nucleotide and amino acid sequence of the Tag sequence is shown below and was originally described by de Boer et al., PNAS 100:7480-85 (2003). Reference: Not provided |
Repositories
| MacDonald Lab | |
|---|---|
 Request this resource
|
Stock #: Ptf1aBiotinTag Availability Notes: Not provided |
Contact Information
| Preferred Contact | |
|---|---|
| Name | Ray MacDonald |
| Institution | University of Texas Southwestern Medical Center |
| Phone | 214-648-1923 |
| raymond.macdonald@utsouthwestern.edu | |
Associated Publications
No publications associated
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