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Protocols (50)
1. Procedure for assessing gene expression in cell subsets from the human fetal pancreas.
2. FACS of Human Islet Cell Types
3. T1D Mouse Necropsy for Pancreas and Related Organs
4. Methods for accurate cell tracking in movies of dividing progenitors
5. Vangl1 immunohistochemistry protocol
6. Modified RNA Synthesis
7. Two-step crosslinking chromatin immunoprecipitation protocol
8. Assessment of vascularization of islet grafts
9. Frozen Section Staining (Indirect Immunofluorescence)
10. Chemical Differentiation of Human Pluripotent Stem Cells to Definitive Endoderm Using a Potent Small Molecule Inhibitor of GSK-3
11. Wholemount Immunofluorescence
12. Manual dissection of E10.5 pancreatic buds
13. Purification of MafA under Denaturing Conditions
14. Directed differentiation of mouse embryonic stem cells to foregut endoderm
15. Directed differentiation of mouse embryonic stem cells to Sox17+ cells
16. Directed differentiation of human embryonic stem cell to foregut endoderm
17. Isolation of different types of adult human pancreatic cells
18. Cell Culture in Three-dimensional Synthetic PEG Hydrogels
19. Generate insulin-secreting islet clusters from human iPS
20. Co-culturing endothelial cell lines with embryonic pancreas explants
21. Culturing Different Endothelial Cell Lines
22. Mouse genotyping
23. Standard mouse embryonic stem cell culture condition
24. Differentiation of hESCs towards pancreatic like glucose-responsive insulin-secreting cells, in vitro
25. Fibronectin and Vitronectin Improve Differentiation of Human Pluripotent Stem Cells to Definitive Endoderm
26. Melton hESC differentiation protocol
27. Pancreas Dissociation for Virus-Mediated Gene Transfer
28. Pancreatic differentiation of hESCs Keller
29. Isolation of Single Human Beta Cells - Identifying Somatic Mutations
30. Using Ezh2 pathway inhibitor to increase pancreatic progenitor cells from endoderm
31. Sander monolayer CyT49 differentiation protocol
32. Procedure for fractionating and isolating cell subsets from human fetal pancreas.
33. FGF2 Semb hESC differentiation protocol
34. Assessment of vascularization of islet grafts via Lectin infusion
35. Isolation of Pancreatic Islets from Zebrafish
36. Whole Mount Immunofluorescence
37. Procedures to assess gene expression changes in mouse fetal pancraetic cell subsets.
38. Procedure for fractionation and gene expression analysis of juvenile human islet cells.
39. Human and Rodent Ocular Transplantation
40. Procedure for identifying chromatin in juvenile human islet cells.
41. Tissue culture protocol for expansion of human gallbladder cells
42. Non-Human Islet Perifusion
43. Procurement, Shipping, and Pathogen Testing of Fetal Pancreata
44. Tissue Culture Protocol for Expansion of Murine Gallbladder Cells
45. Preparing Islets for Transplant and Calculating IEQs
46. Islet Transplantation Procedure
47. Procurement and shipment of human fetal pancreatic fragments
48. Frozen Section Staining_Avidin/Biotin Amplification
49. Frozen Section Staining_Tyramide Signal Amplification
50. Preparation and size-enrichment of secreted proteins from mammalian cells
