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Raphael Scharfmann, Ph.D.

Investigator
INSERM
Faculty Necker

156 Rue de Vaugirard
Paris, F-75015
France

p. +33140615565
f. +33143060443
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Raphael Scharfmann, Ph.D. - Investigator Profile

Type-1 diabetes is caused by an autoimmune destruction of insulin producing beta cells resulting in insulin deficiency. Insulin therapy is unsatisfactory since it does not prevent complications associated to type-1 diabetes. Thus defining new strategies (cell or regenerative therapies) as basis to cure diabetic patients represents a major challenge. Beta cells develop from pancreatic progenitors that proliferate and next differentiate into functional insulin-producing cells. This is a complex process, each step being controlled by specific signals. Theoretically, beta cell mass can be enhanced by: i) activating the proliferation of pancreatic progenitors; ii) activating their differentiation into beta cells; iii) activating the proliferation of beta cells themselves. During the past years, we developed tools based on rodent models to search for signals controlling each step of beta cell development. We also developed strategies to transfer to reconstituted human models, data generated in rodent models. We generated new results and hypotheses concerning signals controlling each step of pancreatic development. We also dissected specific forms of neonatal diabetes in Human, which permits to define new treatments for children with neonatal diabetes.

Scientific Interests/Keywords

Pancreas, beta cells, diabetes, development

Publications

Publication Citation
20009089 Heinis M, Simon MT, Ilc K, Mazure NM, Pouysségur J, Scharfmann R, Duvillié B Oxygen tension regulates pancreatic beta-cell differentiation through hypoxia-inducible factor 1alpha. (2010) Diabetes 59: 662-9 (Added 2012-01-24 14:36:04.809475)
19586915 Filhoulaud G, Guillemain G, Scharfmann R The hexosamine biosynthesis pathway is essential for pancreatic beta cell development. (2009) J Biol Chem 284: 24583-94 (Added 2010-08-03 02:37:34.083816)
19266046 Ravassard P, Emilie Bricout-Neveu , Hazhouz Y, Pechberty S, Mallet J, Czernichow P, Scharfmann R A new strategy to generate functional insulin-producing cell lines by somatic gene transfer into pancreatic progenitors. (2009) PLoS One 4: e4731 (Added 2012-01-17 15:18:12.138297)
19197155 Haumaitre C, Lenoir O, Scharfmann R Directing cell differentiation with small-molecule histone deacetylase inhibitors: the example of promoting pancreatic endocrine cells. (2009) Cell Cycle 8: 536-44 (Added 2010-08-03 02:37:34.091718)
18958289 Scharfmann R, Xiao X, Heimberg H, Mallet J, Ravassard P Beta cells within single human islets originate from multiple progenitors. (2008) PLoS One 3: e3559 (Added 2013-02-15 15:36:19.950126)
18710955 Haumaitre C, Lenoir O, Scharfmann R Histone deacetylase inhibitors modify pancreatic cell fate determination and amplify endocrine progenitors. (2008) Mol Cell Biol 28: 6373-83 (Added 2012-01-24 14:35:32.383474)
18243096 Xu X, D'Hoker J, Stangé G, Bonné S, De Leu N, Xiao X, Van de Casteele M, Mellitzer G, Ling Z, Pipeleers D, Bouwens L, Scharfmann R, Gradwohl G, Heimberg H Beta cells can be generated from endogenous progenitors in injured adult mouse pancreas. (2008) Cell 132: 197-207 (Added 2013-02-15 15:36:36.946741)
17376780 Guillemain G, Filhoulaud G, Da Silva-Xavier G, Rutter GA, Scharfmann R Glucose is necessary for embryonic pancreatic endocrine cell differentiation. (2007) J Biol Chem 282: 15228-37 (Added 2010-08-03 02:38:46.777415)
17322477 Attali M, Stetsyuk V, Basmaciogullari A, Aiello V, Zanta-Boussif MA, Duvillie B, Scharfmann R Control of beta-cell differentiation by the pancreatic mesenchyme. (2007) Diabetes 56: 1248-58 (Added 2010-08-03 02:38:46.78201)
16885549 Babenko AP, Polak M, Cavé H, Busiah K, Czernichow P, Scharfmann R, Bryan J, Aguilar-Bryan L, Vaxillaire M, Froguel P Activating mutations in the ABCC8 gene in neonatal diabetes mellitus. (2006) N Engl J Med 355: 456-66 (Added 2010-08-03 02:38:46.786006)
16505219 Duvillié B, Attali M, Bounacer A, Ravassard P, Basmaciogullari A, Scharfmann R The mesenchyme controls the timing of pancreatic beta-cell differentiation. (2006) Diabetes 55: 582-9 (Added 2012-01-17 15:19:10.709866)
16134081 Castaing M, Duvillié B, Quemeneur E, Basmaciogullari A, Scharfmann R Ex vivo analysis of acinar and endocrine cell development in the human embryonic pancreas. (2005) Dev Dyn 234: 339-45 (Added 2010-08-03 02:38:46.793772)