Roland Stein, Ph.D.

Professor
Vanderbilt University
Molecular Physiology & Biophysics

723 Light Hall 2215 Garland Avenue
Nashville, TN 37232-0615
USA

p. 615-322-7026
· email
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Roland Stein, Ph.D. - Investigator Profile

Our principal objective is to identify and characterize transcription factors essential in pancreatic islet development and function, with a special focus on those of the insulin gene. Pancreatic beta-cell-type-specific and glucose-induced insulin expression appears to be principally regulated by the PDX-1, MafA, and NeuroD1/BETA2 transcription factors. Individuals heterozygous for an inactivating mutation in PDX-1 and BETA2 are diabetic, presumably due to beta cells no longer producing sufficient insulin to meet the body''s needs.

Scientific Interests/Keywords

Control of islet beta cell development and function

Publication	Citation
12297313	Petersen HV, Jensen JN, Stein R, Serup P Glucose induced MAPK signalling influences NeuroD1-mediated activation and (2002) FEBS Lett 528: 241-5 (Added 2005-04-13 12:59:52)
12052878	Samaras SE, Cissell MA, Gerrish K, Wright CV, Gannon M, Stein R Conserved sequences in a tissue-specific regulatory region of the pdx-1 gene mediate transcription in Pancreatic beta cells: role for hepatocyte nuclear factor 3 beta and Pax6. (2002) Mol Cell Biol 22: 4702-13 (Added 2011-08-22 14:46:01.255254)
11904435	Shih DQ, Heimesaat M, Kuwajima S, Stein R, Wright CV, Stoffel M Profound defects in pancreatic beta-cell function in mice with combined (2002) Proc Natl Acad Sci U S A 99: 3818-23 (Added 2005-04-13 13:04:41)
11756538	Qiu Y, Guo M, Huang S, Stein R Insulin gene transcription is mediated by interactions between the p300 (2002) Mol Cell Biol 22: 412-20 (Added 2005-04-13 13:04:54)
11590182	Gerrish K, Cissell MA, Stein R The role of hepatic nuclear factor 1 alpha and PDX-1 in transcriptional (2001) J Biol Chem 276: 47775-84 (Added 2005-04-13 12:59:55)
11309378	Matsuoka T, Zhao L, Stein R The DNA binding activity of the RIPE3b1 transcription factor of insulin (2001) J Biol Chem 276: 22071-6 (Added 2005-04-13 12:59:44)

Resource ID	Name	Contributed on	Primary Contributor
228	Mafa^fl/+ or Mafa^lox/w	Apr 29, 2008	Yes
1068	Gene expression profiling of MafA, MafB, and MafA/MafB Mutants in E18.5 pancreas	Feb 9, 2009	Yes
2982	Purification of MafA under Denaturing Conditions	Feb 15, 2010	Yes
4076	Pdx1^AIVd	Mar 29, 2011	Yes
4077	Pdx1^(AIVd)Rwst	Mar 29, 2011	Yes
4570	Procurement and shipment of human fetal pancreatic fragments	Mar 5, 2013
6 completed resources viewable