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Doug Melton, Ph.D.

Principal Investigator
Harvard / HHMI
Stem Cell and Regenerative Biology

7 Divinity Avenue, Fairchild 358B
Cambridge, MA 02138
USA

p. 617-495-1812
f. 617-495-2918
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Doug Melton, Ph.D. - Investigator Profile

Research in our laboratory is focused on the developmental biology of the pancreas. We wish to understand how the pancreas normally develops and use that information to grow and develop pancreatic cells (Islets of Langerhans) in culture. These goals challenge us to understand the precursors or stem cells that give rise to the pancreas and to characterize the key gene products that specify cell fates and functions during organogenesis. To this end, we use several vertebrate organisms, including frogs, chickens, and zebrafish, but the majority of our studies are done with mice. We use a wide variety of techniques including functional genomics and gene arrays for gene discovery, tissue explants and grafting for analyzing inductive signals, and developmental genetics for direct assays of gene function. The aim of all our experiments is to understand the genes and cells that direct pancreatic organogenesis. For more information on our research and group members, see the Melton Lab Homepage.

Scientific Interests/Keywords

Microarray, Mouse/Human Stem Cell, Embryonic Development, Islet Cells, Pancreas Development

Principal Investigator, Melton Lab

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Publication Citation
15625329 Melton DA, Daley GQ, Jennings CG Altered nuclear transfer in stem-cell research - a flawed proposal. (2004) N Engl J Med 351: 2791-2 (Added 2012-01-17 15:19:37.554538)
15326371 Dor Y, Melton DA How important are adult stem cells for tissue maintenance? (2004) Cell Cycle 3: 1104-6 (Added 2005-04-13 12:56:50)
15148312 Anneren C, Cowan CA, Melton DA The Src family of tyrosine kinases is important for embryonic stem cell (2004) J Biol Chem 279: 31590-8 (Added 2005-04-13 12:56:44)
15129273 Dor Y, Brown J, Martinez OI, Melton DA Adult pancreatic beta-cells are formed by self-duplication rather than (2004) Nature 429: 41-6 (Added 2005-04-13 12:56:52)
14660441 Gu G, Wells JM, Dombkowski D, Preffer F, Aronow B, Melton DA Global expression analysis of gene regulatory pathways during endocrine pancreatic development. (2004) Development 131: 165-79 (Added 2011-08-19 10:08:00.988174)
14657333 Murtaugh LC, Stanger BZ, Kwan KM, Melton DA Notch signaling controls multiple steps of pancreatic differentiation. (2003) Proc Natl Acad Sci U S A 100: 14920-5 (Added 2005-04-13 12:57:00)
12814555 Lammert E, Gu G, McLaughlin M, Brown D, Brekken R, Murtaugh LC, Gerber HP, Ferrara N, Melton DA Role of VEGF-A in vascularization of pancreatic islets. (2003) Curr Biol 13: 1070-4 (Added 2005-04-13 12:57:05)
12778164 Cleaver O, Melton DA Endothelial signaling during development. (2003) Nat Med 9: 661-8 (Added 2005-04-13 12:57:07)
12636919 Chiang MK, Melton DA Single-cell transcript analysis of pancreas development. (2003) Dev Cell 4: 383-93 (Added 2005-04-13 12:57:10)
12532008 Rajagopal J, Anderson WJ, Kume S, Martinez OI, Melton DA Insulin staining of ES cell progeny from insulin uptake. (2003) Science 299: 363 (Added 2005-04-13 12:57:13)
12490294 Gu G, Brown JR, Melton DA Direct lineage tracing reveals the ontogeny of pancreatic cell fates (2003) Mech Dev 120: 35-43 (Added 2005-04-13 12:57:15)
12228720 Ramalho-Santos M, Yoon S, Matsuzaki Y, Mulligan RC, Melton DA "Stemness": transcriptional profiling of embryonic and adult stem cells. (2002) Science 298: 597-600 (Added 2005-04-13 13:06:53)
12086925 Scearce LM, Brestelli JE, McWeeney SK, Lee CS, Mazzarelli J, Pinney DF, Pizarro A, Stoeckert CJ, Clifton SW, Permutt MA, Brown J, Melton DA, Kaestner KH Functional genomics of the endocrine pancreas: the pancreas clone set and PancChip, new resources for diabetes research. (2002) Diabetes 51: 1997-2004 (Added 2011-04-19 12:44:37.68382)
11973276 Gu G, Dubauskaite J, Melton DA Direct evidence for the pancreatic lineage: NGN3+ cells are islet (2002) Development 129: 2447-57 (Added 2005-04-13 13:07:11)
11934148 Fishman MP, Melton DA Pancreatic lineage analysis using a retroviral vector in embryonic mice (2002) Int J Dev Biol 46: 201-7 (Added 2005-04-13 13:07:13)
11577200 Lammert E, Cleaver O, Melton D Induction of pancreatic differentiation by signals from blood vessels. (2001) Science 294: 564-7 (Added 2005-04-13 12:53:34)
11377827 Huang H, Vogel SS, Liu N, Melton DA, Lin S Analysis of pancreatic development in living transgenic zebrafish embryos. (2001) Mol Cell Endocrinol 177: 117-24 (Added 2005-04-13 13:07:16)
11230152 Grapin-Botton A, Majithia AR, Melton DA Key events of pancreas formation are triggered in gut endoderm by ectopic (2001) Genes Dev 15: 444-54 (Added 2005-04-13 12:57:18)
9651252 Henry GL, Melton DA Mixer, a homeobox gene required for endoderm development. (1998) Science 281: 91-6 (Added 2005-04-13 12:57:45)
Resource ID Name Contributed on Primary Contributor
186 Pdx-cre-ERTM Mar 7, 2006 Yes
189 STOCK-Tg(Pdx1-cre)1Dam/Ucd Mar 7, 2006 Yes
190 ngn3-Cre Mar 7, 2006 Yes
1077 E11.5 Mouse Endodermal Organ Gene Expression Feb 9, 2009 Yes
1078 Comparison of endocrine enriched genes in islet beta cells versus induced beta cells Feb 9, 2009 Yes
4138 Melton hESC differentiation protocol Nov 4, 2011 Yes
4222 Modified RNA Synthesis Jan 18, 2012 Yes
4571 Rosa-lox-stop-lox-M3-cherry Mar 7, 2013
4572 Rosa-TetO-lox-stop-lox-M3-cherry (stronger expression) Mar 7, 2013
9 completed resources viewable