Login · Supported browsers · Citing the BCBC · Contact · Version 4.11
. .

Paul Gadue, Ph.D.

Assistant Professor
Children''s Hospital of Philadelphia
Pathology

3501 Civic Center Blvd, CTRB 5012
Philadelphia, PA 19104
USA

p. 267-426-9607
f. 215-590-3660
· email
· website

Paul Gadue, Ph.D. - Co-investigator Profile

My laboratory studies cell fate decisions, focusing on endoderm and mesoderm specification using mouse and human ES cells and iPS cells. ES/iPS cells can differentiate into all cell types in the body and can be propagated in culture almost indefinitely, generating a virtually unlimited number of cells. These unique characteristics lead to the exciting prospect of using these cells to study disease processes and developmental pathways in vitro and eventually to treat a wide variety of diseases using cell replacement therapies.

One focus of my laboratory is to study a unique endodermal stem cell population that we have generated from human ES and iPS cells. Endoderm stem cells have the ability to be expanded in culture like ES cells and have the capability to generate many endoderm derived tissues such as liver, pancreas and intestine. Importantly, pancreatic beta cells derived from endoderm stem cells are monohormonal and are glucose responsive in vitro.  We are studying the signaling and transcriptional pathways which regulate endoderm stem cell generation and maintenance. We are also utilizing the endodermal stem cell population as a model to study pancreatic beta cell  specification with the goal of generating functional beta cells from human ES and iPS cells.

Scientific Interests/Keywords

ES cells, iPS cells, In vitro differentiation

Publication Citation
23452824 Cheng X, Tiyaboonchai A, Gadue P Endodermal stem cell populations derived from pluripotent stem cells. (2013) Curr Opin Cell Biol : (Added 2013-03-08 12:00:15.02927)
23260146 Li Z, Gadue P, Chen K, Jiao Y, Tuteja G, Schug J, Li W, Kaestner KH Foxa2 and H2A.Z mediate nucleosome depletion during embryonic stem cell differentiation. (2012) Cell 151: 1608-16 (Added 2013-03-08 12:00:21.03167)
23149977 Sommer AG, Rozelle SS, Sullivan S, Mills JA, Park SM, Smith BW, Iyer AM, French DL, Kotton DN, Gadue P, Murphy GJ, Mostoslavsky G Generation of human induced pluripotent stem cells from peripheral blood using the STEMCCA lentiviral vector. (2012) J Vis Exp : (Added 2013-03-08 12:00:24.956944)
22482503 Cheng X, Ying L, Lu L, Galvão AM, Mills JA, Lin HC, Kotton DN, Shen SS, Nostro MC, Choi JK, Weiss MJ, French DL, Gadue P Self-renewing endodermal progenitor lines generated from human pluripotent stem cells. (2012) Cell Stem Cell 10: 371-84 (Added 2013-03-14 17:02:07.332252)
21537085 Christodoulou C, Longmire TA, Shen SS, Bourdon A, Sommer CA, Gadue P, Spira A, Gouon-Evans V, Murphy GJ, Mostoslavsky G, Kotton DN Mouse ES and iPS cells can form similar definitive endoderm despite differences in imprinted genes. (2011) J Clin Invest 121: 2313-25 (Added 2013-03-08 12:00:41.040407)
21119691 Cheng X, Gadue P Liver regeneration from induced pluripotent stem cells. (2010) Mol Ther 18: 2044-5 (Added 2012-03-09 17:02:23.205069)
20715179 Somers A, Jean JC, Sommer CA, Omari A, Ford CC, Mills JA, Ying L, Sommer AG, Jean JM, Smith BW, Lafyatis R, Demierre MF, Weiss DJ, French DL, Gadue P, Murphy GJ, Mostoslavsky G, Kotton DN Generation of transgene-free lung disease-specific human induced pluripotent stem cells using a single excisable lentiviral stem cell cassette. (2010) Stem Cells 28: 1728-40 (Added 2011-03-15 14:49:52.101734)
20008934 Xu J, Watts JA, Pope SD, Gadue P, Kamps M, Plath K, Zaret KS, Smale ST Transcriptional competence and the active marking of tissue-specific enhancers by defined transcription factors in embryonic and induced pluripotent stem cells. (2009) Genes Dev 23: 2824-38 (Added 2011-03-15 14:49:52.116309)
19522011 Gadue P, Gouon-Evans V, Cheng X, Wandzioch E, Zaret KS, Grompe M, Streeter PR, Keller GM Generation of monoclonal antibodies specific for cell surface molecules expressed on early mouse endoderm. (2009) Stem Cells 27: 2103-13 (Added 2011-03-15 14:49:52.137237)
18371422 Nostro MC, Cheng X, Keller GM, Gadue P Wnt, activin, and BMP signaling regulate distinct stages in the developmental pathway from embryonic stem cells to blood. (2008) Cell Stem Cell 2: 60-71 (Added 2012-03-09 17:06:42.461414)
18037879 Irion S, Luche H, Gadue P, Fehling HJ, Kennedy M, Keller G Identification and targeting of the ROSA26 locus in human embryonic stem cells. (2007) Nat Biotechnol 25: 1477-82 (Added 2011-03-15 14:50:07.844738)
17086172 Gouon-Evans V, Boussemart L, Gadue P, Nierhoff D, Koehler CI, Kubo A, Shafritz DA, Keller G BMP-4 is required for hepatic specification of mouse embryonic stem cell-derived definitive endoderm. (2006) Nat Biotechnol 24: 1402-11 (Added 2011-03-15 14:50:07.856251)
17077151 Gadue P, Huber TL, Paddison PJ, Keller GM Wnt and TGF-beta signaling are required for the induction of an in vitro model of primitive streak formation using embryonic stem cells. (2006) Proc Natl Acad Sci U S A 103: 16806-11 (Added 2011-03-15 14:50:07.866297)
No viewable resources have been contributed