Guoqiang Gu, Ph.D.

Associate Professor
Vanderbilt University
Department of Cell and Developmental Biology

4130B MRB III 465 21st Avenue South
Nashville, TN 37232-8240
USA

p. 615-936-3634
f. 615-343-4539
· email

Guoqiang Gu, Ph.D. - Investigator Profile

We use mouse and chicken embryos as models to study what factors are required for endocrine islet differentiation and cellular/functional maintenance. We identify factors that are sufficient to induce endocrine differentiation in model organisms and then determine whether they induce islet production from non-beta cells in postnatal mice. Typical projects in the lab include: 1) determine the expression patterns of candidate genes in pancreatic tissues, determine the effect of expressing these candidate genes in chicken embryos; 2) characterize the pancreatic phenotypes in mouse mutants; 3) create gene knockout and knockin constructs; and 4) using combinatorial cell lineage marking to examine/identify the competence factors that direct selection of particular islet cell types from endocrine progenitors; and 5) examining the molecules/growth factors that facilitate endocrine cell production from none-endocrine islet cells in regenerattion models.

Scientific Interests/Keywords

Endocirne islet cell differentiation and functional maintenance, adult pancreatic progenitor during regeneration, cell marking and cell lineage

Publication	Citation
20622165	Zhao A, Ohara-Imaizumi M, Brissova M, Benninger RK, Xu Y, Hao Y, Abramowitz J, Boulay G, Powers AC, Piston D, Jiang M, Nagamatsu S, Birnbaumer L, Gu G Gαo represses insulin secretion by reducing vesicular docking in pancreatic beta-cells. (2010) Diabetes 59: 2522-9 (Added 2011-03-12 10:23:43.753158)
20025861	Wang S, Yan J, Anderson DA, Xu Y, Kanal MC, Cao Z, Wright CV, Gu G Neurog3 gene dosage regulates allocation of endocrine and exocrine cell fates in the developing mouse pancreas. (2010) Dev Biol 339: 26-37 (Added 2011-03-12 10:25:30.437094)
19487660	Wang S, Jensen JN, Seymour PA, Hsu W, Dor Y, Sander M, Magnuson MA, Serup P, Gu G Sustained Neurog3 expression in hormone-expressing islet cells is required for endocrine maturation and function. (2009) Proc Natl Acad Sci U S A 106: 9715-20 (Added 2009-12-16 15:32:09.160363)
18543299	Means AL, Xu Y, Zhao A, Ray KC, Gu G A CK19(CreERT) knockin mouse line allows for conditional DNA recombination in epithelial cells in multiple endodermal organs. (2008) Genesis 46: 318-23 (Added 2009-12-16 15:32:40.508848)
18394599	Wang S, Hecksher-Sorensen J, Xu Y, Zhao A, Dor Y, Rosenberg L, Serup P, Gu G Myt1 and Ngn3 form a feed-forward expression loop to promote endocrine islet cell differentiation. (2008) Dev Biol 317: 531-40 (Added 2009-12-16 15:33:18.595756)
17893102	Xu Y, Xu G, Liu B, Gu G Cre reconstitution allows for DNA recombination selectively in dual-marker-expressing cells in transgenic mice. (2007) Nucleic Acids Res 35: e126 (Added 2009-12-16 15:34:40.511419)
16920096	Xu Y, Wang S, Zhang J, Zhao A, Stanger BZ, Gu G The fringe molecules induce endocrine differentiation in embryonic endoderm by activating cMyt1/cMyt3. (2006) Dev Biol 297: 340-9 (Added 2009-12-16 15:37:12.838119)

Resource ID	Name	Contributed on	Primary Contributor
226	Myt1flox	Mar 4, 2008	Yes
229	Myt1Flox	Apr 22, 2008	Yes
1037	Global expression analysis of gene regulatory pathways during endocrine pancreatic development	Apr 9, 2004	Yes
2301	Ngn3^nCre	Oct 28, 2009	Yes
2482	Polyclonal Mouse Ngn3 raised in Goat	Nov 23, 2009
2642	Mouse genotyping	Dec 16, 2009	Yes
2662	Manual dissection of E10.5 pancreatic buds	Dec 16, 2009	Yes
4059	Ngn3^HA.LCA	Mar 23, 2011	Yes
4078	Nkx2.2^{cCre-Knock-in}	Apr 1, 2011	Yes
4079	Nkx2.2^{(cCre-Knock-in)Ggu}	Apr 1, 2011	Yes
4117	Transcriptional Profiling of Ngn3-dependent endocrine progenitors and pre-beta cells	Aug 2, 2011	Yes
4123	Pax4^HA.LCA	Aug 5, 2011	Yes
4145	Ngn3^(HA.LCA)Ggu	Nov 21, 2011	Yes
4146	Pax4^(HA.LCA)Ggu	Nov 21, 2011	Yes
4333	Transcriptional profiling of nascent and mature pancreatic beta cells	Apr 24, 2012	Yes
4335	Transcriptional profiling of Ngn3-dependent pancreatic endocrine differentiation at E15.5	Apr 24, 2012	Yes
4489	Ngn3^T2A.nCre	Jun 22, 2012	Yes
4527	Nkx2.2^T2A.cCre	Jul 10, 2012	Yes
4538	Ngn3^T2A.nCre	Sep 13, 2012	Yes
4556	Nkx2.2^T2A.cCre	Oct 18, 2012	Yes
20 completed resources viewable