Ole Madsen, Ph.D. - Investigator Profile
I have a longstanding interest in processes controlling beta-cell maturation. We have for several years studied pluripotent islet tumor cells - from which destinct hormone producing islet tumors could be derived from common clonal origin. More recently, we have studied processes of pancreas and islet beta-cell ontogeny. We are much interested in the signal transduction processes controlling pancreatic endocrine maturation. Much of our current focus relates to the translational aspect of developmental biology to coax pluripotent stem cell differentiation towards therapeutic beta cells for diabetes treatment.
Our main focus with the BCBC is to generate novel antibody tools for important factors/markers for the different stages of pancreatic maturation. Such reagents will be made available through the Antibody Core.
Scientific Interests/Keywords
antibody, cell surface, epitope, extracellular, cell lineage tracing, fluorescent cells, antibody tags, cell sorting, cell separation, cell culture, antibody mediated cell purification, protocol development.
Affiliates
Publications
| PubMedID | Citation |
|---|---|
| 20558340 | Galbo T, Pedersen IL, Fløyel T, Bang-Berthelsen CH, Serup P, Madsen OD, Hald J. Novel monoclonal antibodies against Pdx1 reveal feedback regulation of Pdx1 protein levels. (2010) Eur J Histochem 54: e19 |
| 20336589 | Hansson M, Madsen OD. Pluripotent stem cells, a potential source of beta-cells for diabetes therapy. (2010) Curr Opin Investig Drugs 11: 417-25 |
| 19665969 | Collombat P, Xu X, Ravassard P, Sosa-Pineda B, Dussaud S, Billestrup N, Madsen OD, Serup P, Heimberg H, Mansouri A. The ectopic expression of Pax4 in the mouse pancreas converts progenitor cells into alpha and subsequently beta cells. (2009) Cell 138: 449-62 |
| 19213833 | Holst B, Egerod KL, Jin C, Petersen PS, Østergaard MV, Hald J, Sprinkel AM, Størling J, Mandrup-Poulsen T, Holst JJ, Thams P, Orskov C, Wierup N, Sundler F, Madsen OD, Schwartz TW. G protein-coupled receptor 39 deficiency is associated with pancreatic islet dysfunction. (2009) Endocrinology 150: 2577-85 |
| 18845151 | Gustavsen CR, Chevret P, Krasnov B, Mowlavi G, Madsen OD, Heller RS. The morphology of islets of Langerhans is only mildly affected by the lack of Pdx-1 in the pancreas of adult Meriones jirds. (2008) Gen Comp Endocrinol 159: 241-9 |
| 18665014 | Klein T, Heremans Y, Heimberg H, Pipeleers D, Madsen OD, Serup P, Heller RS. Investigation and characterization of the duct cell-enriching process during serum-free suspension and monolayer culture using the human exocrine pancreas fraction. () Pancreas 38: 36-48 |
| 18347078 | Hald J, Sprinkel AE, Ray M, Serup P, Wright C, Madsen OD. Generation and characterization of Ptf1a antiserum and localization of Ptf1a in relation to Nkx6.1 and Pdx1 during the earliest stages of mouse pancreas development. (2008) J Histochem Cytochem 56: 587-95 |
| 18212389 | Klinck R, Serup P, Madsen OD, Jørgensen MC. Specificity of four monoclonal anti-NKx6-1 antibodies. (2008) J Histochem Cytochem 56: 415-24 |
| 17881611 | Jørgensen MC, Ahnfelt-Rønne J, Hald J, Madsen OD, Serup P, Hecksher-Sørensen J. An illustrated review of early pancreas development in the mouse. (2007) Endocr Rev 28: 685-705 |
| 17631449 | Madsen OD. Pancreas phylogeny and ontogeny in relation to a 'pancreatic stem cell'. (2007) C R Biol 330: 534-7 |
| 17478445 | Ahnfelt-Rønne J, Jørgensen MC, Hald J, Madsen OD, Serup P, Hecksher-Sørensen J. An improved method for three-dimensional reconstruction of protein expression patterns in intact mouse and chicken embryos and organs. (2007) J Histochem Cytochem 55: 925-30 |
| 17160042 | Madsen OD, Serup P. Towards cell therapy for diabetes. (2006) Nat Biotechnol 24: 1481-3 |
| 17053071 | Narendran P, Neale AM, Lee BH, Ngui K, Steptoe RJ, Morahan G, Madsen O, Dromey JA, Jensen KP, Harrison LC. Proinsulin is encoded by an RNA splice variant in human blood myeloid cells. (2006) Proc Natl Acad Sci U S A 103: 16430-5 |
| 16984985 | Hainerova I, Torekov SS, Ek J, Finkova M, Borch-Johnsen K, Jorgensen T, Madsen OD, Lebl J, Hansen T, Pedersen O. Association between neuromedin U gene variants and overweight and obesity. (2006) J Clin Endocrinol Metab 91: 5057-63 |
| 16480455 | Madsen OD. Stem cells and diabetes treatment. (2005) APMIS 113: 858-75 |
| 16401696 | Pedersen IL, Klinck R, Hecksher-Sorensen J, Zahn S, Madsen OD, Serup P, Jorgensen MC. Generation and characterization of monoclonal antibodies against the transcription factor Nkx6.1. (2006) J Histochem Cytochem 54: 567-74 |
| 16122727 | Heller RS, Jenny M, Collombat P, Mansouri A, Tomasetto C, Madsen OD, Mellitzer G, Gradwohl G, Serup P. Genetic determinants of pancreatic epsilon-cell development. (2005) Dev Biol 286: 217-24 |
| 15983231 | Torekov SS, Larsen LH, Glumer C, Borch-Johnsen K, Jorgensen T, Holst JJ, Madsen OD, Hansen T, Pedersen O. Evidence of an association between the Arg72 allele of the peptide YY and increased risk of type 2 diabetes. (2005) Diabetes 54: 2261-5 |
| 15684667 | Zahn S, Pedersen J, Serup IL, Madsen P. Generation of monoclonal antibodies against mouse neurogenin 3: a new immunocytochemical tool to study the pancreatic endocrine progenitor cell. (2004) Hybrid Hybridomics 23: 385-8 |
| 15543206 | Rescan C, Lefebvre S, Frandsen VH, Klein U, Foschi T, Pipeleers M, Scharfmann DG, Madsen R, Heimberg OD. EGF-induced proliferation of adult human pancreatic duct cells is mediated by the MEK/ERK cascade. (2005) Lab Invest 85: 65-74 |
| 15031110 | Heller RS, Stoffers DA, Liu A, Schedl A, Crenshaw EB, Madsen OD, Serup P. The role of Brn4/Pou3f4 and Pax6 in forming the pancreatic glucagon cell identity. (2004) Dev Biol 268: 123-34 |
| 14686787 | Heller RS, Klein T, Ling Z, Heimberg H, Katoh M, Madsen OD, Serup P. Expression of Wnt, Frizzled, sFRP, and DKK genes in adult human pancreas. (2003) Gene Expr 11: 141-7 |
| 12921743 | Hald J, Hjorth JP, German MS, Madsen OD, Serup P, Jensen J. Activated Notch1 prevents differentiation of pancreatic acinar cells and attenuate endocrine development. (2003) Dev Biol 260: 426-37 |
| 12754281 | Klein T, Ling Z, Heimberg H, Madsen OD, Heller RS, Serup P. Nestin is expressed in vascular endothelial cells in the adult human pancreas. (2003) J Histochem Cytochem 51: 697-706 |
| 12412008 | Heller RS, Dichmann DS, Jensen J, Miller C, Wong G, Madsen OD, Serup P. Expression patterns of Wnts, Frizzleds, sFRPs, and misexpression in transgenic mice suggesting a role for Wnts in pancreas and foregut pattern formation. (2002) Dev Dyn 225: 260-70 |
| 12403815 | Heremans Y, Gradwohl M, Serup P, Madsen G, Pipeleers P, Heimberg O. Recapitulation of embryonic neuroendocrine differentiation in adult human pancreatic duct cells expressing neurogenin 3. (2002) J Cell Biol 159: 303-12 |
| 11423476 | Heller RS, Stoffers DA, Bock T, Svenstrup K, Jensen J, Horn T, Miller CP, Habener JF, Madsen OD, Serup P. Improved glucose tolerance and acinar dysmorphogenesis by targeted (2001) Diabetes 50: 1553-61 |
| 11141151 | Serup P, Madsen OD, Mandrup-Poulsen T. Islet and stem cell transplantation for treating diabetes. (2001) BMJ 322: 29-32 |
| 10969830 | Ammendrup A, Maillard A, Nielsen K, Aabenhus Andersen N, Serup P, Dragsbaek Madsen O, Mandrup-Poulsen T, Bonny C. The c-Jun amino-terminal kinase pathway is preferentially activated by (2000) Diabetes 49: 1468-76 |
| 10891400 | Petersen HV, Jorgensen MC, Andersen FG, Jensen J, F-Nielsen T, Jorgensen R, Madsen OD, Serup P. Pax4 represses pancreatic glucagon gene expression. (2000) Mol Cell Biol Res Commun 3: 249-54 |
| 10868931 | Jensen J, Heller RS, Funder-Nielsen T, Pedersen EE, Lindsell C, Weinmaster G, Madsen OD, Serup P. Independent development of pancreatic alphaand beta-cells from (2000) Diabetes 49: 163-76 |
| 10825208 | Jacquemin P, Durviaux SM, Jensen J, Godfraind C, Gradwohl G, Guillemot F, Madsen OD, Carmeliet P, Dewerchin M, Collen D, Rousseau GG, Lemaigre FP. Transcription factor hepatocyte nuclear factor 6 regulates pancreatic (2000) Mol Cell Biol 20: 4445-54 |
| 10628902 | Tyrberg B, Eizirik DL, Marklund SL, Olejnicka B, Madsen OD, Andersson A. Human islets in mixed islet grafts protect mouse pancreatic beta-cells from alloxan toxicity. (1999) Pharmacol Toxicol 85: 269-75 |
| 10615124 | Jensen J, Pedersen EE, Galante P, Hald J, Heller RS, Ishibashi M, Kageyama R, Guillemot F, Serup P, Madsen OD. Control of endodermal endocrine development by Hes-1. (2000) Nat Genet 24: 36-44 |
| 10580420 | Nielsen K, Karlsen AE, Deckert M, Madsen OD, Serup P, Mandrup-Poulsen T, Nerup J. Beta-cell maturation leads to in vitro sensitivity to cytotoxins. (1999) Diabetes 48: 2324-32 |
| 10567713 | Jørgensen MC, Vestergård Petersen H, Ericson J, Madsen OD, Serup P. Cloning and DNA-binding properties of the rat pancreatic beta-cell-specific factor Nkx6.1. (1999) FEBS Lett 461: 287-94 |
| 10424881 | Tingstedt JE, Edlund H, Madsen OD, Larsson LI. Gastric amylin expression. Cellular identity and lack of requirement for the homeobox protein PDX-1. A study in normal and PDX-1-deficient animals with a cautionary note on antiserum evaluation. (1999) J Histochem Cytochem 47: 973-80 |
| 10375461 | Luna G, Francini F, Gómez Dumm CL, Madsen O, Gagliardino JJ. Hormone coexpression in the adult toad endocrine pancreas: double-label immunofluorescence under basal conditions and after glucose injection. (1999) Gen Comp Endocrinol 115: 29-36 |
| 10214934 | Jensen PB, Kristensen P, Clausen JT, Judge ME, Hastrup S, Thim L, Wulff BS, Foged C, Jensen J, Holst JJ, Madsen OD. The hypothalamic satiety peptide CART is expressed in anorectic and non-anorectic pancreatic islet tumors and in the normal islet of Langerhans. (1999) FEBS Lett 447: 139-43 |
| 10094480 | Andersen FG, Jensen J, Heller RS, Petersen HV, Larsson LI, Madsen OD, Serup P. Pax6 and Pdx1 form a functional complex on the rat somatostatin gene upstream enhancer. (1999) FEBS Lett 445: 315-20 |
| 10094478 | Andersen FG, Heller RS, Petersen HV, Jensen J, Madsen OD, Serup P. Pax6 and Cdx2/3 form a functional complex on the rat glucagon gene promoter G1-element. (1999) FEBS Lett 445: 306-10 |
| 9870681 | Azzoni C, D'Adda T, Tamburrano G, Coscelli C, Madsen OD, Scopsi L, Bordi C. Functioning human insulinomas. An immunohistochemical analysis of intracellular insulin processing. (1998) Virchows Arch 433: 495-504 |
| 9714543 | Petersen HV, Peshavaria M, Pedersen AA, Philippe J, Stein R, Madsen OD, Serup P. Glucose stimulates the activation domain potential of the PDX-1 homeodomain transcription factor. (1998) FEBS Lett 431: 362-6 |
| 9603781 | Oster A, Jensen J, Serup P, Galante P, Madsen OD, Larsson LI. Rat endocrine pancreatic development in relation to two homeobox gene products (Pdx-1 and Nkx 6.1). (1998) J Histochem Cytochem 46: 707-15 |
| 9590691 | Kristensen P, Judge ME, Thim L, Ribel U, Christjansen KN, Wulff BS, Clausen JT, Jensen PB, Madsen OD, Vrang N, Larsen PJ, Hastrup S. Hypothalamic CART is a new anorectic peptide regulated by leptin. (1998) Nature 393: 72-6 |
| 9435324 | Jensen PB, Blume N, Mikkelsen JD, Larsen PJ, Jensen HI, Holst JJ, Madsen OD. Transplantable rat glucagonomas cause acute onset of severe anorexia and adipsia despite highly elevated NPY mRNA levels in the hypothalamic arcuate nucleus. (1998) J Clin Invest 101: 503-10 |
| 9230347 | Madsen OD, Jensen J, Petersen HV, Pedersen EE, Oster A, Andersen FG, Jørgensen MC, Jensen PB, Larsson LI, Serup P. Transcription factors contributing to the pancreatic beta-cell phenotype. (1997) Horm Metab Res 29: 265-70 |
| 9025070 | Larsson LI, Madsen OD, Serup P, Jonsson J, Edlund H. Pancreatic-duodenal homeobox 1 -role in gastric endocrine patterning. (1996) Mech Dev 60: 175-84 |
| 9022666 | Madsen OD, Jensen J, Blume N, Petersen HV, Lund K, Karlsen C, Andersen FG, Jensen PB, Larsson LI, Serup P. Pancreatic development and maturation of the islet B cell. Studies of pluripotent islet cultures. (1996) Eur J Biochem 242: 435-45 |
| 8799146 | Serup P, Jensen J, Andersen FG, Jørgensen MC, Blume N, Holst JJ, Madsen OD. Induction of insulin and islet amyloid polypeptide production in pancreatic islet glucagonoma cells by insulin promoter factor 1. (1996) Proc Natl Acad Sci U S A 93: 9015-20 |
| 8702531 | Jensen J, Serup P, Karlsen C, Nielsen TF, Madsen OD. mRNA profiling of rat islet tumors reveals nkx 6.1 as a beta-cell-specific homeodomain transcription factor. (1996) J Biol Chem 271: 18749-58 |
| 8692940 | Heimberg H, De Vos A, Moens K, Quartier E, Bouwens L, Pipeleers D, Van Schaftingen E, Madsen O, Schuit F. The glucose sensor protein glucokinase is expressed in glucagon-producing alpha-cells. (1996) Proc Natl Acad Sci U S A 93: 7036-41 |
| 8501199 | Jensen CH, Teisner B, Højrup P, Rasmussen HB, Madsen OD, Nielsen B, Skjødt K. Studies on the isolation, structural analysis and tissue localization of fetal antigen 1 and its relation to a human adrenal-specific cDNA, pG2. (1993) Hum Reprod 8: 635-41 |
| 8421685 | Deltour L, Leduque P, Blume N, Madsen O, Dubois P, Jami J, Bucchini D. Differential expression of the two nonallelic proinsulin genes in the developing mouse embryo. (1993) Proc Natl Acad Sci U S A 90: 527-31 |
| 8404649 | Madsen OD, Karlsen C, Nielsen E, Lund K, Kofod H, Welinder B, Rehfeld JF, Larsson LI, Steiner DF, Holst JJ. The dissociation of tumor-induced weight loss from hypoglycemia in a transplantable pluripotent rat islet tumor results in the segregation of stable alpha- and beta-cell tumor phenotypes. (1993) Endocrinology 133: 2022-30 |
| 8148038 | Lund K, Blume N, Michelsen BK, Bucchini D, Madsen OD. Differential expression of non-allelic insulin genes in rodent islet tumour cells. (1993) J Mol Endocrinol 11: 305-18 |
| 7937976 | Petersen HV, Serup P, Leonard J, Michelsen BK, Madsen OD. Transcriptional regulation of the human insulin gene is dependent on the homeodomain protein STF1/IPF1 acting through the CT boxes. (1994) Proc Natl Acad Sci U S A 91: 10465-9 |
| 7652479 | Madsen OD, Karlsen C, Blume N, Jensen HI, Larsson LI, Holst JJ. Transplantable glucagonomas derived from pluripotent rat islet tumor tissue cause severe anorexia and adipsia. (1995) Scand J Clin Lab Invest Suppl 220: 27-35 |
| 7593609 | Blume N, Skouv J, Larsson LI, Holst JJ, Madsen OD. Potent inhibitory effects of transplantable rat glucagonomas and insulinomas on the respective endogenous islet cells are associated with pancreatic apoptosis. (1995) J Clin Invest 96: 2227-35 |
| 7575438 | Serup P, Petersen HV, Pedersen EE, Edlund H, Leonard J, Petersen JS, Larsson LI, Madsen OD. The homeodomain protein IPF-1/STF-1 is expressed in a subset of islet cells and promotes rat insulin 1 gene expression dependent on an intact E1 helix-loop-helix factor binding site. (1995) Biochem J 310 ( Pt 3): 997-1003 |
| 7540573 | Karlsen AE, Andersen HU, Vissing H, Larsen PM, Fey SJ, Cuartero BG, Madsen OD, Petersen JS, Mortensen SB, Mandrup-Poulsen T. Cloning and expression of cytokine-inducible nitric oxide synthase cDNA from rat islets of Langerhans. (1995) Diabetes 44: 753-8 |
| 6207064 | Madsen OD, Frank BH, Steiner DF. Human proinsulin-specific antigenic determinants identified by monoclonal antibodies. (1984) Diabetes 33: 1012-6 |
| 6196183 | Madsen OD, Cohen RM, Fitch FW, Rubenstein AH, Steiner DF. The production and characterization of monoclonal antibodies specific for human proinsulin using a sensitive microdot assay procedure. (1983) Endocrinology 113: 2135-44 |
| 3896518 | Orci L, Ravazzola M, Amherdt M, Madsen O, Vassalli JD, Perrelet A. Direct identification of prohormone conversion site in insulin-secreting cells. (1985) Cell 42: 671-81 |
| 3537136 | Contreas G, Madsen OD, Vissing H, Lernmark A. A simple assay for the detection of antibodies to endocrine islet cell surface antigens. (1986) J Immunol Methods 95: 135-9 |
| 3536964 | Orci L, Ravazzola M, Amherdt M, Madsen O, Perrelet A, Vassalli JD, Anderson RG. Conversion of proinsulin to insulin occurs coordinately with acidification of maturing secretory vesicles. (1986) J Cell Biol 103: 2273-81 |
| 3516766 | Madsen OD, Olsson ML, Bille G, Sundkvist G, Lernmark A, Dahlqvist G, Ludvigsson J. A two-colour immunofluorescence test with a monoclonal human proinsulin antibody improves the assay for islet cell antibodies. (1986) Diabetologia 29: 115-8 |
| 2877997 | Madsen OD, Larsson LI, Rehfeld JF, Schwartz TW, Lernmark A, Labrecque AD, Steiner DF. Cloned cell lines from a transplantable islet cell tumor are heterogeneous and express cholecystokinin in addition to islet hormones. (1986) J Cell Biol 103: 2025-34 |
| 2842785 | Madsen OD, Andersen LC, Michelsen B, Owerbach D, Larsson LI, Lernmark A, Steiner DF. Tissue-specific expression of transfected human insulin genes in pluripotent clonal rat insulinoma lines induced during passage in vivo. (1988) Proc Natl Acad Sci U S A 85: 6652-6 |
| 2805585 | Contreas G, Dyrberg T, Madsen OD, Markholst H, Lernmark A. Increased levels of circulating immune complexes are not associated with diabetes in BB rats. (1989) Diabetes Res 10: 109-13 |
| 2663499 | Larsson LI, Nielsen JH, Hutton JC, Madsen OD. Pancreatic hormones are expressed on the surfaces of human and rat islet cells through exocytotic sites. (1989) Eur J Cell Biol 48: 45-51 |
| 2644530 | Nielsen JH, Linde S, Welinder BS, Billestrup N, Madsen OD. Growth hormone is a growth factor for the differentiated pancreatic beta-cell. (1989) Mol Endocrinol 3: 165-73 |
| 2644126 | Bucchini D, Madsen O, Desbois P, Pictet R, Jami J. B islet cells of pancreas are the site of expression of the human insulin gene in transgenic mice. (1989) Exp Cell Res 180: 467-74 |
| 2550269 | Beck TC, Madsen OD. Monoclonal antibodies as probes to the differentiated exocrine pancreas react to monoclonal islet tumor tissue. (1989) Exp Clin Endocrinol 93: 255-60 |
| 2443408 | Madsen OD. Proinsulin-specific monoclonal antibodies. Immunocytochemical application as beta-cell markers and as probes for conversion. (1987) Diabetes 36: 1203-11 |
| 2274051 | Fromont-Racine M, Bucchini D, Madsen O, Desbois P, Linde S, Nielsen JH, Saulnier C, Ripoche MA, Jami J, Pictet R. Effect of 5'-flanking sequence deletions on expression of the human insulin gene in transgenic mice. (1990) Mol Endocrinol 4: 669-77 |
| 2199167 | Madsen OD, Contreas G, Jørgensen J. Monoclonal (auto)antibodies in insulin-dependent diabetes mellitus. (1990) Curr Top Microbiol Immunol 156: 75-86 |
| 2169420 | Nielsen E, Welinder B, Madsen OD. Protein HMG-17 is hyper-expressed in rat glucagonoma. Single-step isolation and sequencing. (1990) Eur J Biochem 192: 81-6 |
| 2122446 | Contreas G, Jørgensen J, Madsen OD. Novel islet, duct, and acinar cell markers defined by monoclonal autoantibodies from prediabetic BB rats. (1990) Pancreas 5: 540-7 |
| 1989847 | Fürnsinn C, Komjati M, Madsen OD, Schneider B, Waldhäusl W. Lifelong sequential changes in glucose tolerance and insulin secretion in genetically obese Zucker rats (fa/fa) fed a diabetogenic diet. (1991) Endocrinology 128: 1093-9 |
| 1954895 | Nielsen E, Welinder BS, Madsen OD. Chromogranin-B, a putative precursor of eight novel rat glucagonoma peptides through processing at mono-, di-, or tribasic residues. (1991) Endocrinology 129: 3147-56 |
| 1924335 | Michelsen BK, Petersen JS, Boel E, Møldrup A, Dyrberg T, Madsen OD. Cloning, characterization, and autoimmune recognition of rat islet glutamic acid decarboxylase in insulin-dependent diabetes mellitus. (1991) Proc Natl Acad Sci U S A 88: 8754-8 |
| 1850107 | Madsen OD, Nielsen JH, Michelsen B, Westermark P, Betsholtz C, Nishi M, Steiner DF. Islet amyloid polypeptide and insulin expression are controlled differently in primary and transformed islet cells. (1991) Mol Endocrinol 5: 143-8 |
| 1734887 | De Vroede M, Foriers A, Van de Winkel M, Madsen O, Pipeleers D. Presence of islet amyloid polypeptide in rat islet B and D cells determines parallelism and dissociation between rat pancreatic islet amyloid polypeptide and insulin content. (1992) Biochem Biophys Res Commun 182: 886-93 |
| 1569972 | Blume N, Petersen JS, Andersen LC, Kofod H, Dyrberg T, Michelsen BK, Serup P, Madsen OD. Immature transformed rat islet beta-cells differentially express C-peptides derived from the genes coding for insulin I and II as well as a transfected human insulin gene. (1992) Mol Endocrinol 6: 299-307 |
| 1563584 | In 't Veld PA, Zhang F, Madsen OD, Klöppel G. Islet amyloid polypeptide immunoreactivity in the human fetal pancreas. (1992) Diabetologia 35: 272-6 |
| 1406710 | Møller CJ, Christgau S, Williamson MR, Madsen OD, Niu ZP, Bock E, Baekkeskov S. Differential expression of neural cell adhesion molecule and cadherins in pancreatic islets, glucagonomas, and insulinomas. (1992) Mol Endocrinol 6: 1332-42 |
