http://www.betacell.org/news/ News and Events of the Beta Cell Biology Consortium Thu, 21 Mar 2013 15:11:16 +0000 en-us Copyright 2013, Beta Cell Biology Consortium http://www.betacell.org/news/index.php?view=469 http://www.betacell.org/news/index.php?view=469 Thu, 21 Mar 2013 00:00:00 +0000 Jorge Ferrer (IDIBAPS and Imperial College London) Genomic Regulation of Pancreatic B-Cells Topics: Beta Cell Targets and T2D Epigenetic Programming Beta Cell Regeneration Beta Cell Differentiation Beta Cell Growth Beta cells and alpha cells Non-coding RNAs   http://www.betacell.org/news/index.php?view=429 http://www.betacell.org/news/index.php?view=429 Tue, 26 Feb 2013 00:00:00 +0000 Genome Browser tracks (coverage plots and peaks) and relevant gene lists are now available for this study. Epigenetics of human pancreatic cells (Magnuson lab). Public access (previously BCBC-only) The aim of this study is to investigate the cell-type-specific, genome-wide H3K4me3 and H3K27me3 profiles and transcriptional landscapes of human alpha-, beta-, and exocrine cells using ChIP-Seq and RNA-Seq analysis. ChIP-Seq analysis of enhancer related histone modifications during hESC differentiation towards pancreatic endoderm (Sander lab). BCBC-only The objective of these experiments was to employ ChIP-Seq to map changes in enhancer related chromatin modifications during CyT49 hESC differentiation towards the pancreatic lineage. Such data will be used to identify enhancer elements during pancreatic lineage specification of hESCs. Miscellaneous (7) AAV targeting vectors (BCBC access) Confirm Enrichment of Neurogenin 3 - Expressing Cells Using Cell Surface Markers (BCBC access) Procedures for Isolating and Characterizing the Function of Juvenile Human Islets (BCBC access) Procedures for Procuring and Shipping NHP Fetal Pancreatic Fragments (BCBC access) Reprogramming of human pancreatic exocrine cells (BCBC access) TetO HA Pdx1 plasmid (BCBC access) TetO Myc HNF6 plasmid (BCBC access) Mouse Strains (6) Nkx2.2{T2A.cCre} (BCBC access) NOD-Rag IL2rγnull Ins2Akita (BCBC access) NOD-scid IL2Rγ{null} (BCBC access) Pdx1.H2B.Apple (BCBC access) Rosa26{228.3TF.GFP-Cre} (BCBC access) Rosa26{mIre1.N906A.Cherry} (BCBC access) Protocols (5) Procedure for assessing gene expression in cell subsets from the human fetal pancreas. (BCBC access) Procedure for fractionating and isolating cell subsets from human fetal pancreas. (BCBC access) Procedure for fractionation and gene expression analysis of juvenile human islet cells. (BCBC access) Procedure for identifying chromatin in juvenile human islet cells. (BCBC access) Procedures to assess gene expression changes in mouse fetal pancraetic cell subsets. (BCBC access) http://www.betacell.org/news/index.php?view=409 http://www.betacell.org/news/index.php?view=409 Tue, 19 Feb 2013 00:00:00 +0000 http://www.betacell.org/news/index.php?view=369 http://www.betacell.org/news/index.php?view=369 Mon, 07 Jan 2013 00:00:00 +0000 http://www.betacell.org/news/index.php?view=349 http://www.betacell.org/news/index.php?view=349 Thu, 20 Dec 2012 00:00:00 +0000 http://www.betacell.org/news/index.php?view=329 http://www.betacell.org/news/index.php?view=329 Tue, 20 Nov 2012 00:00:00 +0000 If you have any issue please contact us). For example: Heatmaps are now a view on gene summary pages. Gene unique identifiers contain the species and gene symbol (note that this implies that if you had bookmarked gene pages, you will need to regenerate those bookmarks). New functionality to facilitate downloads of very large results set is now in place. Updated BCBC Workgroup workspaces for membership and other changes: Bioinformatics and Epigenomics Workgroup (now inactive, but access to document remains). In vitro generation of glucose-responsive beta cells Workgroup. New Reagents Workgroup. Added new resource type for Adeno-Associated Virus resources. We are in the process of annotating resources. Improvements made to dkCOIN data fetcher on resource pages, in order to prevent time-outs, due to transition of dkCOIN from Vanderbilt to the Amazon EC2 cloud computing environment. Fixed contributions report in dashboard. Fixed (funded) TRGP projects that were not appearing in public research pages. Improvement to SPOT and navigation options. Fixed incorrect use of "Edit Resource" in genomics study pages (request an edit instead). New and Updated Resources Antibodies (8) Polyclonal Mouse Ngn3 raised in Chicken (BCBC access) Polyclonal Mouse Ngn3 raised in Chicken (BCBC access) Polyclonal Mouse Nkx6.1 raised in Chicken (BCBC access) Polyclonal Mouse Nkx6.1 raised in Chicken (BCBC access) Polyclonal Mouse PDX1 raised in Chicken (BCBC access) Polyclonal Mouse PDX1 raised in Chicken (BCBC access) Polyclonal Mouse Prox1 raised in Chicken (BCBC access) Polyclonal Mouse Prox1 raised in Chicken (BCBC access) Mouse ESC Lines (9) Foxa2{LCA} (BCBC access) Neurog3{Cre-ER.BAC} (BCBC access) Neurog3{RG.BAC} (BCBC access) Ngn3{T2A.nCre} (BCBC access) Nkx2.2{T2A.cCre} (BCBC access) Pdx1{H2B.Neptune} (BCBC access) Rosa26{228.3TF.GFP.Cre} (BCBC access) Rosa26{mIre1.N906A.Cherry} (BCBC access) Sst.rTTA.LCA (BCBC access) Genomics Studies (3) Transcriptional and epigenetic high throughput sequencing of human and mouse pancreatic islet-cells" (Moran et al., Cell Metab., 2012). Public access The aim of this study was to obtain a detailed transcriptional landscape of human and mouse islet-cells, including strand information, as well an epigenetic map of transcriptionally active chromatin. Genome Browser tracks (coverage plots and peaks), a list of novel non-coding RNAs, and inferred gene relationships for POLR2A based on ChIP-seq are available for this study. Transcriptional profiling of pancreatic developmental intermediates: definitive endoderm, foregut endoderm and pre-pancreatic progenitor cells (Magnuson Lab). BCBC access This study used RNA-Seq technology to examine transcription profiles of pancreatic developmental intermediates: 1) definitive endoderm (Sox17^GFP/+ [het] cells at E8.5, 8-10 somites); 2) foregut endoderm (Pdx1^CFP/+(het) cells at E9.5) and 3) Ptf1a-positive and Ptf1a-deficient pre-pancreatic endoderm progenitor cells (Ptf1a^YFP/+ (het) cells and Ptf1a^YFP/ YFP (ko) cells at E10.5). Whole transcriptome analysis of pancreatic ghrelin cells in WT and Nkx2.2 KO mouse embryo (Sussel Lab). BCBC access Whole transcriptome analysis of Ghrelin-expressing and -descendant cells in pancreas from Nkx2.2 KO and WT mouse embryos. Miscellaneous (16) Bioactivity of Endothelial Secreted Protein Subfractions on Ngn3 and Beta cell Development (BCBC access) Define apical‐polarity and cell‐cell adhesive status of newborn beta‐cells during their transition. From Ngn3+ progenitors to Insulin‐positive beta‐cells (immunofluorescence microscopy). (BCBC access) Define the lineage potential of Rac1-deficient Ngn+ cells (BCBC access) Define whether Rac1 in beta‐cell differentiation works autonomously or non‐autonomously, using traceable mosaic Rac1 ablation via intercrosses with Pdx1CreER and R26YFPR strains (BCBC access) Effects of blocking delamination of newborn beta-cells by dominant-active Cdc42 (BCBC access) Estimation of Depth of Somatic Mutations in Human Beta Cells. (BCBC access) Ferrer lncRNA manuscript - confidential (BCBC access) Generation of a Transgenic Line to Visualize Beta-cell Proliferation (BCBC access) Identification of 20 Molecules that Enhance Beta-Cell Regeneration in Zebrafish (BCBC access) Identification of the Reprogramming Target Cells in Murine Gall Bladder Cells (BCBC access) Immunodetection of PCP factors (other access) List of GPCR's Expressed at Significant Levels in Human Islets (BCBC access) PCP gene Celsr3 controls beta cell differentiation (other access) Processing of Fetal Human and NHP Pancreatic Tissue for Transplantation (BCBC access) Quantification of planes of divisions of progenitors in vivo (other access) Transplantation assay (preliminary results) to evaluate the potential of hESC-derived populations (BCBC access) Mouse Strain (7) Ghrl{Cre-eGFP} (BCBC access) Ngn3{T2A.nCre} (BCBC access) Pdx1{Cre} (BCBC access) Pdx1{rtTA} (BCBC access) Rag2{-/-}gamma-c{-/-}Ngn3{GFP/+} (BCBC access) Rosa26{228.3TF.GFP-Cre} (BCBC access) Rosa26{228.TOP.CFP} (BCBC access) Protocol (1) Procurement, Shipping, and Pathogen Testing of Fetal Pancreata (BCBC access) http://www.betacell.org/news/index.php?view=309 http://www.betacell.org/news/index.php?view=309 Wed, 31 Oct 2012 00:00:00 +0000 http://www.betacell.org/news/index.php?view=289 http://www.betacell.org/news/index.php?view=289 Thu, 11 Oct 2012 00:00:00 +0000 It is not intended to support research focused on understanding normal biology, disease processes, or generating lists of putative new targets. At the end of the project period, a successful project will have provided a significant contribution to the data supporting the validity of modulating a target’s activity for safe, efficacious treatment of a disease using a small molecule or non-viral biologic approach. Scientific/Research Contact at NIDDK Aaron C. Pawlyk, Ph.D. Program Director for Pharmacogenomics and Drug Discovery National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) Telephone:  301-451-7299 Email:  pawlykac@mail.nih.gov http://www.betacell.org/news/index.php?view=249 http://www.betacell.org/news/index.php?view=249 Tue, 07 Aug 2012 00:00:00 +0000 For example, view all resources associated with mouse Pdx1. Gene Pages Reorganized (Beta Cell Genomics) Gene pages have been reorganized using an accordion format, which makes it easier to separate and view different sections of the pages. Moreover: Links to the Bioimage resources have been added to the gene pages, alongside of other BCBC Resources. A new section has been added that gives information on whether the gene is expressed in different types of pancreatic cells, based on various kinds of evidence, including Beta Cell Genomics expression studies. This addition was prompted by an investigator request at our last BCBC Retreat.  This tool is described in the Visualization Resource page. Search Improvements (Beta Cell Genomics) Search results have been enhanced by: Allowing users to save favorite genes and genomic locations. Providing clouds afd histograms on selected columns of result pages to indicate the distribution of terms and values in the results. Permitting transformations from gene lists to relationships and from relationships to gene lists. Other Improvements (Beta Cell Genomics) KEGG pathways data have been incorporated. A new search has been added to look for genes in a selected pathway. Moreover KEGG pathway information has been added to the gene pages. Login has been improved so that when users logs in they do not lose the strategies that they worked on as guest users. Additianal PWM-based predictions of binding sites are now available for 13 Human and Mouse transcription factors: HES1, HNF1, HNF3, HNF4, ONECUT1, PDX1, NKX6-1, PAX4, PAX6, TCF7L2, NKX2-2, EP300, SOX17. Corresponding tracks have been added to the Genome Browser. Other New Features and Improvements Added a CC "staff alumni" listing on the CC Staff page. Improved antibody uses page and data storage. Eliminated Repostem (legacy library for Vanderbilt/betacell.org). Replaced table widget across all resource pages, which now provides in-table search and better sorting (example). Saving shipping and billing addresses entered in resource request into member profile, should these not exist. Added new text about RGP Funding Program funding status. Improvements to Events Management System for the 2012 BCBC Fall Planning Meeting. Bug fixes Research networks page is now fixed. Resource bioimage thumbnail was overwriting the forwarding destination following authentication request. Rich text editor in Events Management System was fixed. GenBank files were not properly uploading to "Genetic Alterations" widgest in Mouse Strain and Mouse ESC Line resource types. Forcing IE9 to start site in IE9 "mode,", vs. defaulting to IE7 mode. Events Management System registration page for FPM was showing affiliate invitation text, which has been removed. Updated JQuery calls due to upgrade. Loaded missing tags for public genomics studies (that have study summaries). Fixed issue with archiving deleted resources. New and Updated Resources Antibodies (24) Monoclonal Human Cd31 (PECAM1) raised in Mouse (BCBC access) Monoclonal Human Muc1 raised in hamster (armenian) (BCBC access) Monoclonal Rat Tuj1 (TUBB3) raised in Rabbit (BCBC access) Polyclonal Cow GFAP raised in Rabbit (BCBC access) Polyclonal Cow Insulin raised in Guinea Pig (BCBC access) Polyclonal Human CDCP1 raised in Goat (BCBC access) Polyclonal Human Glucagon (GCG) raised in Guinea Pig (BCBC access) Polyclonal Human Glucagon (GCG) raised in Rabbit (BCBC access) Polyclonal Human Glucagon raised in Rabbit (BCBC access) Polyclonal Human HNF-1β raised in Goat (BCBC access) Polyclonal Human Insulin raised in Goat (BCBC access) Polyclonal Human Insulin raised in Guinea Pig (BCBC access) Polyclonal Human Ki67 raised in Rabbit (BCBC access) Polyclonal Human MafA raised in Rabbit (BCBC access) Polyclonal Human MafB raised in Rabbit (BCBC access) Polyclonal Human Pancreatic Polypeptide (PPY) raised in Rabbit (BCBC access) Polyclonal Human Somatostatin (Sst) raised in Goat (BCBC access) Polyclonal Human Somatostatin (Sst) raised in Sheep (BCBC access) Polyclonal Human Sox9 raised in Rabbit (BCBC access) Polyclonal Human α-Amylase (AMY2A) raised in Rabbit (BCBC access) Polyclonal Mouse Foxa2 (HNF3b) raised in Goat (BCBC access) Polyclonal Pig Insulin raised in Guinea Pig (BCBC access) Polyclonal Rat Synaptophysin (SYP) raised in Rabbit (BCBC access) Polyclonal Rat VEGFA raised in Goat (BCBC access) Genomics Studies (2) Pdx1 Cistrome of Pancreatic Islets (Khoo et al., Molecular Endocrinology, 2012) Public access This study used ChIP-seq for genomic location analysis of Pdx1 in human and mouse pancreatic islets. Gene lists and Genome Browser tracks (coverage plots and peaks) are available for this study. Baseline gene expression profile of human extrahepatic biliary cells/biliospheres in vitro BCBC access This study was aimed at determining the baseline gene expression profile of human gallbladder and cystic duct cells at various stages from isolation in fresh biopsy samples to early and later passages in culture. (Grompe Lab) A previously BCBC restricted study has become public: Mouse Sox17.Epi versus Sox17.Endo cells in E9.5 embryos (E. Choi, M.R-C. Kraus, L.A. Lemaire, M. Yoshimoto, S. Vemula, L.A. Potter, E. Manduchi, C.J. Stoeckert Jr., A. Grapin-Botton, M.A. Magnuson, Dual lineage-specific expression of Sox17 during mouse embryogenesis, Stem Cells, in press, 2012.). Gene lists and Genome Browser tracks (coverage plots) are now available for this study. Bioimages (67) G16w Fetal human pancreas development (GCG, INS, PDX1) (BCBC access) G16w Fetal human pancreas development (GCG, INS, PDX1) (BCBC access) G16w Fetal human pancreas development (GFAP, PECAM1, GCG|INS|SST) (BCBC access) G16w Fetal human pancreas development (GFAP, PECAM1, GCG|INS|SST) (BCBC access) G16w Fetal human pancreas development (PDX1, PECAM1, INS) (BCBC access) G16w Fetal human pancreas development (PDX1, PECAM1, INS) (BCBC access) G16w Fetal human pancreas development (PDX1, PECAM1, INS) (BCBC access) G16w Fetal human pancreas development (PDX1, PECAM1, INS) (BCBC access) G16w Fetal human pancreas development (TUBB3, PECAM1, GCG|INS|SST) (BCBC access) G16w Fetal human pancreas development (TUBB3, PECAM1, GCG|INS|SST) (BCBC access) G16w Fetal human pancreas development (TUBB3, PECAM1, GCG|INS|SST) (BCBC access) G16w Fetal human pancreas development (TUBB3, PECAM1, GCG|INS|SST) (BCBC access) G17w Fetal human pancreas development (MKI67, INS, PDX1) (BCBC access) G17w Fetal human pancreas development (MKI67, INS, PDX1) (BCBC access) G18w Fetal human pancreas development (ARX, GCG, INS) (BCBC access) G18w Fetal human pancreas development (ARX, GCG, INS) (BCBC access) G18w Fetal human pancreas development (GCG, INS, SST) (BCBC access) G18w Fetal human pancreas development (GCG, INS, SST) (BCBC access) G18w Fetal human pancreas development (GCG, INS, SST) (BCBC access) G18w Fetal human pancreas development (INS, GCG, SST) (BCBC access) G18w Fetal human pancreas development (INS, GCG, SST) (BCBC access) G18w Fetal human pancreas development (INS, GCG, SST) (BCBC access) G18w Fetal human pancreas development (INS, GCG, SST) (BCBC access) G18w Fetal human pancreas development (INS, GCG, SST) (BCBC access) G18w Fetal human pancreas development (INS, GCG, SST) (BCBC access) G18w Fetal human pancreas development (MAFB, INS, PDX1) (BCBC access) G18w Fetal human pancreas development (MAFB, INS, PDX1) (BCBC access) G18w Fetal human pancreas development (NEUROG3, PAX6|SYP, CDH1) (BCBC access) G18w Fetal human pancreas development (NEUROG3, PAX6|SYP, CDH1) (BCBC access) G18w Fetal human pancreas development (NEUROG3, PAX6|SYP, CDH1) (BCBC access) G18w Fetal human pancreas development (NEUROG3, PAX6|SYP, CDH1) (BCBC access) G18w Fetal human pancreas development (NEUROG3, PAX6|SYP, CDH1) (BCBC access) G18w Fetal human pancreas development (PAX6, INS, GCG) (BCBC access) G18w Fetal human pancreas development (PAX6, INS, GCG) (BCBC access) G18w Fetal human pancreas development (PDX1, INS, GCG) (BCBC access) G18w Fetal human pancreas development (PDX1, INS, GCG) (BCBC access) G18w Fetal human pancreas development (PDX1, INS, GCG) (BCBC access) G18w Fetal human pancreas development (PDX1, INS, GCG) (BCBC access) G18w Fetal human pancreas development (PDX1, INS, GCG) (BCBC access) G18w Fetal human pancreas development (TUBB3, PECAM1, INS) (BCBC access) G7w Fetal human pancreas development (ACE, PDX1, CDH1) (BCBC access) G7w Fetal human pancreas development (CDH5, PDX1, CDH1) (BCBC access) G7w Fetal human pancreas development (F3, PDX1, CDH1) (BCBC access) G7w Fetal human pancreas development (HNF1B, ONECUT1, CDH1) (BCBC access) G7w Fetal human pancreas development (HNF1B, ONECUT1, CDH1) (BCBC access) G7w Fetal human pancreas development (HNF1B, ONECUT1, CDH1) (BCBC access) G7w Fetal human pancreas development (HNF1B, ONECUT1, CDH1) (BCBC access) G7w Fetal human pancreas development (MAFB, GCG, PDX1) (BCBC access) G7w Fetal human pancreas development (MAFB, GCG, PDX1) (BCBC access) G7w Fetal human pancreas development (MAFB, INS, PDX1) (BCBC access) G7w Fetal human pancreas development (MAFB, INS, PDX1) (BCBC access) G7w Fetal human pancreas development (, PAX6, CDH1) (BCBC access) G7w Fetal human pancreas development (PDX1, MKI67, CDH1) (BCBC access) G7w Fetal human pancreas development (PDX1, MKI67, INS) (BCBC access) G7w Fetal human pancreas development (PTF1A, CPA1, CDH1) (BCBC access) G7w Fetal human pancreas development (PTF1A, CPA1, CDH1) (BCBC access) G7w Fetal human pancreas development (PTF1A, FOXA2, CDH1) (BCBC access) G7w Fetal human pancreas development (PTF1A, FOXA2, CDH1) (BCBC access) G7w Fetal human pancreas development (PTF1A, FOXA2, CDH1) (BCBC access) G7w Fetal human pancreas development (SOX17, PDX1, CDH1) (BCBC access) G7w Fetal human pancreas development (SOX18, PDX1, CDH1) (BCBC access) G7w Fetal human pancreas development (Sox19, PDX1, CDH1) (BCBC access) G7w Fetal human pancreas development (SOX9,) (BCBC access) G7w Fetal human pancreas development (SOX9) (BCBC access) G7w Fetal human pancreas development (SOX9) (BCBC access) G8w Fetal human pancreas development (PDX1, GCG, INS) (BCBC access) G8w Fetal human pancreas development (PDX1, GCG, INS) (BCBC access) http://www.betacell.org/news/index.php?view=229 http://www.betacell.org/news/index.php?view=229 Wed, 13 Jun 2012 00:00:00 +0000 Successive subcloning of the newly generated line(s) should be performed in order to confirm that a homogenous cell line has been generated. Assessment of regulated insulin secretion in any newly developed beta cell preparation or beta cell line should be performed using sophisticated physiological assays, such as in a cell perifusion system coupled with challenges with a variety of known beta-cell secretagogues. Function should be assessed at different passage levels to ensure that the phenotype is stable and the cells don’t senesce. While creating a scalable source of human insulin beta cell should be the priority of any proposal that comes in response to this announcement, generating a similar source of human alpha cells could be proposed as a parallel effort. Read the Program Description for additional details and contact information. http://www.betacell.org/news/index.php?view=189 http://www.betacell.org/news/index.php?view=189 Tue, 24 Apr 2012 00:00:00 +0000 The data represents approximately 500 z-stacks and images from 25-30 human fetal pancreas samples. Extensive improvements to the Resource Request System The Resource Request System (RRS) has been rebuilt to make the process of dealing with resource requests as simple and clear as possible for both requesters and suppliers.  The RRS is tied into the dashboard so that requests needing your attention are highlighted.  At each stage of the request process the RRS makes it clear whose turn it is to take action, and what actions are available.  Also, if an affiliate requests a resource the name of their Principal Investigator is included in the request notification, and the Principal Investigator is CC’d on all RRS notifications concerning the request.  Complex and potentially confusing features allowing suppliers to maintain multiple inventory items per resource did not appear to be useful and so have been removed. Improvements to Beta Cell Genomics The Genome Browser has been upgraded to use GBrowse2 (version 2.39), with a more user-friendly interface. New features include: Genome browser tracks have been separated according to species. The user can upload his/her Custom Tracks. Ability to view BCBC restricted tracks upon logging in, in addition to the public study tracks. We have added a basket tool for genes. This allows a user to cherry pick individual genes and place them in the basket for further analysis. Steps in the Strategies can be used to transform lists of one type (e.g., Relationships of genes) to lists of another type (e.g., Genes). The following transformations have been added to the Strategies: Relationship Results to Gene Lists. Heatmap Lists to Gene Lists. Gene Lists to Heatmap Lists. Comparisons to Studies. HTS Analyses to Studies. Look and feel of the meta-analysis heatmaps and comparison profiles have been improved New Resources Genomics Studies (5) Reprogramming of mouse gall bladder cells into pancreatic beta cells BCBC access This study used RNA-seq profiling to study transdifferentiation of gall bladder cells to beta cells. Mouse gall bladder cells from MIP-GFP mice were expanded in vitro and transduced with adenoviral vectors expressing the transcription factors Neurog3, Pdx1 and MafA. Reprogrammed GFP+ cells were then FACS-sorted for RNA isolation. (Grompe Lab) Epigenetics of human pancreatic cells BCBC access RNA-seq and ChIP-Seq profiling (H3K4me3 and H3K27me3) of sorted human pancreatic alpha, beta, and exocrine cells was performed. (Kaestner Lab) Transcriptional profiling of Ngn3-dependent pancreatic endocrine differentiation at E15.5 BCBC access This study used RNA-Seq profiling to explore gene expression in mouse Ngn3^GFP/+ [het] FACS sorted pancreatic cells at E15.5 (committed endocrine progenitor cells) and in Ngn3^GFP/GFP [null] at E15.5 (defective endocrine progenitor cells). This study is designed to understand the gene expression alteration in the endocrine lieage at different embryonic days. The aim is to understand both Ngn3 dependent and independent gene expression profiles so as to reveal the instructive signals that specify the collective endocrine islet cell fate or specific islet cell type. (Gu Lab) ChIP-Seq dataset for Ptf1a ChIP of chromatin from dissected, whole E17.5 mouse pancreas BCBC access ChIP-Seq analysis of chromatin from whole E17.5 mouse pancreas was used to identify the genome-wide sites of bound Ptf1a, RbpjL and RbpJ. Tracks for this study are also visible on our Genome Browser upon logging in. (MacDonald Lab) Transcriptional profiling of nascent and mature pancreatic beta cells BCBC access This study used RNA-Seq profiling to examine transcription profiles in FACS-sorted MIP-EGFP+ mouse pancreatic cells at E16.5 (nascent beta cells) and P60 (mature beta cells). Such an analysis should reveal the gene transcription alterations for beta cell development and for function. (Gu Lab) Protocols(9) Frozen Section Staining_Tyramide Signal Amplification BCBC access Frozen Section Staining_Avadin/Biotin Amplification BCBC access Islet Transplantation Procedure BCBC access Preparing Islets for Transplant and Calculating IEQs BCBC access Tissue Culture Protocol for Expansion of Murine Gallbladder Cells BCBC access Isolation of Single Human Beta Cells – Identifying Somatic Mutations BCBC access Tissue culture protocol for expansion of human gallbladder cells BCBC access Differention of hESCs towards pancreatic like glucose-responsive insulin-secreting cells, in vitro BCBC access Human and Rodent Ocular Transplantation BCBC access http://www.betacell.org/news/index.php?view=143 http://www.betacell.org/news/index.php?view=143 Sun, 25 Mar 2012 00:00:00 +0000